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29 Cards in this Set
- Front
- Back
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Blood agar plates (BAP) grow nearly everything except what 2 species? |
Haemophilus and Neisseria |
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Chocolate plates (CHOC) have which factors that are lacking in BAP? |
Factor X (hemin) and Factor V (NAD). |
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What species needs both factor X and factor V to grow on a blood agar? |
Haemophilus |
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Name 2 plates that Haemophilus can grow on. |
CHOC and Modified Thayer Martin |
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What species needs factor X (hemin) to grow on a blood agar? |
Neisseria (it grows on CHOC and Thayer Martin) |
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Which plate is best for pathogenic Neisseria species? |
Thayer Martin |
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BBE plates are used for growing __? |
Bacteroides |
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HEA plates are used for growing what species? |
Salmonella (turns black) and Shigella (turns metallic-green) |
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SMAC (Sorbitol MacConkey agar) plates are used for growing what species? |
E. coli 0157:H7. This species will turn clear, others will turn pink. |
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CAMPY (Campylobacter Agar) plates are used for growing what species? |
Campylobacter jejuni |
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Campylobacter is grown on specially selective "CAMP" agar plates at what temperature and for how long? |
42C, for 48 hours. |
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LKV (Brucella Laked Blood Agar with Kanamycin and Vancomycin) will grow what species? |
Bacteroides (white). Also Prevotella. |
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BBL plates are used to grow what? |
Yeasts. C. albicans turns green, C. tropicalis turns metallic blue, and C. krusei turns pink/fuzzy. Others turn white or mauve. |
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Which half of a urine plate should be streaked first? |
The BAP side. Always streak from least selective to most selective. |
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LJ tubes grow what organism? |
Mycobacterium, like T.B., which appear brown and granular. This takes 4 weeks.
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LIM broth is used to grow what organism? |
Group B. strep. It inhibits other vaginal flora. |
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What is the gram, catalase, and lactose fermentation for Staph aureus? |
G+ Cat+ LF |
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What is the gram, catalase for Strep pyogenes? |
G+ Cat- |
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What is the gram, catalase, oxi and indole reaction for Haemophilus influenzae? |
G- Cat+ Oxi+ Ind VAR |
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What is the gram, catalase, and hemolysis for Strep agalactiae? |
G+ Cat- Hem Beta |
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What is the gram, oxi, indole, and lactose fermentation for E. coli? |
G- Oxi- Ind+ LF |
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What is the gram, catalase, and hemolysis for Strep pneumoniae? |
G+ Cat- Hem ALPHA |
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What is the gram, PYR and LAP for Enterococcus? |
G+ PYR+ LAP+ |
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What is the gram, catalase, hemolysis for Viridans streptococci? |
G+ Cat- Hem ALPHA |
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What is the gram, catalase, LAP and PYR for Staph lugdenensis? |
G+ Cat+ LAP+ PYR+ |
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What is the gram, catalase, oxi, indole, lactose fermentation and hemolysis for Pseudomonas aeruginosa? |
G- Cat+ Oxi+ Ind- NLF Hem BETA! |
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What two yeasts both have germ tubes?
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C. albicans and C. dubliniensis |
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Germ tubes are like hyphae that have no __ at their point of origin. |
constriction |
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What is the gram, lactose fermentation, hemolysis, oxi and indole reaction for Klebsiella? |
G- LF Hem GAMMA Oxi- Ind- (EXCEPT for K. oxytoca) Indo- |
