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19 Cards in this Set

  • Front
  • Back
a collection of DNA sequences from one source is a
genetic library
the clones in the genetic library are a resource for work on
specific genes
clones for a specific gene can be recovered from a library by using _______ to screen the library
what is a radioactive-labeled nucleic acid, either DNA or RNA that contain a base sequence complementary to all or part of the gene of interest
both _____ and _____ lables are used in a genetic library
chemical, radioactive
how does the probe work
Dna must be denatured into single strands

a prepared single-stranded labeled probe must be mixed with the denatured DNA
the probe forms a hybrid via a
complementary base segment
if the gene being sought is expressed in certain cells, the mRNA from those cells can be used to make a
what is another way to produce a large number of copies of a specific DNA sequence
PCR (Polymerase Chain Reaction) technique (1986)
1st step of PCR:

Denatured DNA is separated into
2nd step of PCR:

Annealing primers for DNA replication are added and bind to complementary regions on the _______.

These primers are synthesized in lab and are ______ long
single-stranded DNA's.

20-30 nucleotides
3rd Step of PCR:

The enzyme DNA polymerase begins at the primer and synthesizes a DNA strand complementary to the region between the
step 3 of PCR is called
primer extension
PCR doubles the amount of ______ after each cycle
genetic material
After n cyles of PCR, there is a ______ increase in the amount of double-stranded DNA
to be capable of joining together of complementary nucleotides (heating and cooling)
used to link the covalent bonds between the DNA fragments
enzyme DNA ligase
Enzyme DNA ligase is used to link the covalent bonds betweenthe DNA fragments. This is a
recombinant DNA molecule
transfer DNA from one cell to another