Microbiology Identifying And Analyzing Cloned Sequences Notes

Front 1

a collection of DNA sequences from one source is a

Back 1

genetic library

Front 2

the clones in the genetic library are a resource for work on

Back 2

specific genes

Front 3

clones for a specific gene can be recovered from a library by using _______ to screen the library

Back 3

Probes

Front 4

what is a radioactive-labeled nucleic acid, either DNA or RNA that contain a base sequence complementary to all or part of the gene of interest

Back 4

probe

Front 5

both _____ and _____ lables are used in a genetic library

Back 5

chemical, radioactive

Front 6

how does the probe work

Back 6

Dna must be denatured into single strands

a prepared single-stranded labeled probe must be mixed with the denatured DNA

Front 7

the probe forms a hybrid via a

Back 7

complementary base segment

Front 8

if the gene being sought is expressed in certain cells, the mRNA from those cells can be used to make a

Back 8

probe

Front 9

what is another way to produce a large number of copies of a specific DNA sequence

Back 9

PCR (Polymerase Chain Reaction) technique (1986)

Front 10

1st step of PCR:

Denatured DNA is separated into

Back 10

strands

Front 11

2nd step of PCR:

Annealing primers for DNA replication are added and bind to complementary regions on the _______.

These primers are synthesized in lab and are ______ long

Back 11

single-stranded DNA's.

20-30 nucleotides

Front 12

3rd Step of PCR:

The enzyme DNA polymerase begins at the primer and synthesizes a DNA strand complementary to the region between the

Back 12

primers

Front 13

step 3 of PCR is called

Back 13

primer extension

Front 14

PCR doubles the amount of ______ after each cycle

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genetic material

Front 15

After n cyles of PCR, there is a ______ increase in the amount of double-stranded DNA

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2^n

Front 16

to be capable of joining together of complementary nucleotides (heating and cooling)

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anneal

Front 17

used to link the covalent bonds between the DNA fragments

Back 17

enzyme DNA ligase

Front 18

Enzyme DNA ligase is used to link the covalent bonds betweenthe DNA fragments. This is a

Back 18

recombinant DNA molecule

Front 19

transfer DNA from one cell to another

Back 19

Transformation