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497 Cards in this Set

  • Front
  • Back
what is a G+ ovoid organims that is more resistant than streptococci?
enterococcus faecalis
what is an important secondary invader as a cause of human disease?
enterococcus faecalis
what is the first, second, and third most common cause of nocosomial infections?
1-E. Coli
2-Staphylococcus
3-Enterococcus
what are the 5 clinical manifestation for Enterococcus?
nocosomial infection
UTI
endocarditis
bacteremia
neontal infections
what bacteria is distinguished from streptococci with a positive bile-esculin test?
enterococcus faecalis
T/F lactobacillus infections are caused by exogenous organisms
false
-endogenous
which bacterial produces lactic acid from sucrose?
lactobacillus
what is the optimal pH for lactobacillus?
3.5-4.5
what bacteria can be found in the mouth, stomach, and female genital tract?
lactobacillus
what bacteria appears to be important in smooth surface caries?
lactobacillus
what bacteria is important in caries that have progressed into the dentin of the tooth?
lactobacillus
what bacteria can be used as an indicator organism for risk assessment for the development of dental caries?
lactobacillus
what are the most common sites of colonization for corynebacteria?
skin and nasopharynx
which toxin prevents protein synthesis and kills affected cells?
diptheria
which infection has pseudomembrane formation?
corynebacterium diphtheriae
what is the route of transmission for corynbacteria diphtheriae?
droplets
what vaccine is a modified toxin (toxoid)?
diptheria
which G+ bacteria has "whip handle" morphology?
Corynebacterium matruchotii
which bacteria is isolated from dental plaque and comprises the central filament of "corn cob formations"?
Corynebacterium matruchotii
what bacteria does C. matruchotii bind to at the salivary interface of supragingival plaque?
S. sanguis
which bacteria are anaerobic diphtheroids?
propionibacterium
which bacteria are G+ diptheroid (club shaped)?
propionibacterium
proprionibacterium produce _____ as a major by-product of carbohydrate metabolism.
propionic acid
what are some of the virulence factors for propionibacterium?
*lipase
protease
neuraminidase
hyaluronidase
which bacteria's virulence factor stimulate an inflammatory reaction in humans?
propionibacterium
what is a member of the climax community, and is an early colonizer?
propionibacterium
which bacteria attaches to streptococcus gordonii and fusobacterium nucleatum?
propionibacterium
which bacteria is involved in root caries and acne?
propionibacterium acnes
what G+ bacteria has airborne tranmission?
mycobacteria
which bacteria is acid fast?
mycobacteria
T/F mycobacteria are closer to G- than G+
false
-more similar to G+
what are the major pathogens for mycobacteria?
M. tuberculosis
M. leprae
M. avium-intracellualare complex (MAC)
which bacteria may show filamentous and/or club shape?
M. tuberculosis
for mycobacteria what is responsible for the acid fastness?
mycolic acids
what causes the bacterium to grow in filaments?
cord factor
which bacteria has a long generation time (8-10 days)?
M. tuberculosis
what are the 3 routes of entry for M. tuberculosis?
respiratory, ingestion, cutaneous
what is the most common form of M. tuberculosis and accounts for 90% of deaths?
pulmonary form
what is the walled off lesion that occurs with M. tuberculosis?
tubercle
what are the symptoms of M. tuberculosis?
fatigue, anorexia, weight loss, low grade fever that persists for months
T/F M. tuberculosis transmission is primary airborne
true
which bacteria have a low infectious dose with less than 10 organisms?
M. tuberculosis
which skin test uses purified protein derivative (PPD) and a positive test is redness, swelling, and hardening?
M. tuberculosis
what are two types of tuberculin testing?
tuberculin skin test
interferon gamma release assay
what is the gold standard for M tuberculosis diagnosis?
culture
what are 4 diagnostic test you can use for M. tuberculosis?
AFB smear
microscope
PCR
DNA probe
what are the four human pathogens in Actinomyces?
A. israelii
A. naeslundii
A. viscosus
A. odontolyticus
which bacteria has sulfur granules and mycelial-like structures?
Actinomyces
T/F actinomyces is a degenerative disease
true
T/F actinomyces produces many toxins
false
-it produces no toxins
what is the cell mediated response that produces tubercle like lesions?
granulomatous response
what bacteria will you see granules and look for G+ nonacid fast diphtheriods?
Actinomyces
what is the treatment for Actinomyces infection?
drainage
surgical excision
penicillin *
what bacteria has been isolated from human plaque, calculus, and tonsillar crypts?
actinomyces israelii
what is the most common associated bacteria with human actinomycosis?
A. israelii
is A israelii an early or late colonizer?
early
which bacteria is found in dental plaque but a specific relationship to disease has not been established?
A. odontolyticus
which bacteria colonizes the tongue and has fibrils?
A. naeslundii
the type 1 fibrils attach to?
salivary pellicle
the type 2 fibrils adheres to?
mucosal surface
oral streptococci
what is the ligand that is revealed by removing sialic acid by neurominidase?
galactosides
what is the enzyme that cleaves sialic acid?
neurominidase
neurominidase cleaves?
sialic acid
what bacteria has neurominidase enzyme?
A. naeslundii
which bacteria colonizes teeth and causes human gingivitis but NOT chronic periodontitis?
A. viscosus
what 2 bacteria have typ e 1 and type 2 fibrils?
A. naeuslundii
A. viscosus
which G+, partially acid fast bacteria that occurs in soil?
Nocardia (actinomyces)
what G+ bacteria is isolated from dental plaque from deep dental carious lesions(3rd degree dental caries)?
Bifidobacterium (actinomyces)
what g+ bacteria occurs in root surface plaque and is associated with endocarditis?
rothia dentocariosa
what type of bacteria form spores?
Clostridia
what members of the group cause severe infection of muscle tissue?
Clostridia
what infection requires lowered oxidation reduction potentials?
Clostridia
what bacteria occurs in muscle damage and impaired blood supply?
Clostridium perfringens
what are the 3 clinical manifestations for Clostridium perfringens?
superficial contamination
clostridial cellulitis
gas gangrene
what is associated with toxemia, extensive local edema, foul smelling exudate, gas, and massive tissue damage?
gas gangrene
what is the essential factor predisposing to wound infection with C. perfringens?
trauma
what 3 diagnostic tests to use for C. perfringens?
gram stain
culture
Naegler test
which bacteria can infect once other anaerobic flora is eliminated allowing overgrowth?
C. difficile
what bacteria has a high mortality rate (27-44%) if untreated?
C. difficile
which bacteria can cause diarrhea, colitis, or pseudomembrane colitis?
C. difficile
which bacteria has the neurotoxin(tetanospasmin)?
C. tetani
which bacteria is non invasive and remains confined to necrotic tissue, symptoms occur when toxin diffuses into bloodstream?
C. tetani
which bacteria blocks release of neurotransmitters like glycine?
C. tetani
which bacteria has a toxin that prevents contraction of antagonistic muscle, results in spasms and convulsions?
Clostridium tetani
what are the initial signs of Clostridium tetani?
muscle stiffness
lockjaw
T/F immunization for C. tetani is with a toxoid
true
what is one of the most potent poisons known?
neurotoxin A
what bacteria produces neurotoxin A?
C. botulinum
what bacteria can interfere with the release of acetycholine?
C. botulinum
T/F muscle spasms are a result of Clostridium botulinum
False
-flaccid muscle paralysis
T/F food borne botulism is usually a result of inadequate sterilization of home canned foods
true
what is a g+, obligate anaerobe associated with aggressive and chronic periodontitis?
Eubacterium
what bacteria can cause intrabdominal anerobic infections?
Bacteroides fragilis
which bacteria is NOT a black pigmenting bacteria that is the most resistant of all anaerobes to antimicrobial agents?
Bacteriodes fragilis
what are the virulence factors for Bacteriodes fragilis?
neuraminidase,
hyaluronidase
deozyribonucleases
heparinase
which G- bacteria is associated with aggressive periodontitis and abscesses of the periodontium?
Tannerella forsythensis
which bacteria have been found in subgingival and supragingival biofilms and serve as reservoirs for recolonization?
Tannerella forsythensis
what is a g- bacteria that can cause rapidly progressive periodontitis?
porphyromonas gingivalis
which G- is associated with chronic and agressive periodontitis and periodontal abscess, highest numbers occur in the gingival crevice?
P. gingivalis
what are the virulence factors for P. gingivalis?
endotoxin** most important
capsule
fimbriae
collagenase
hyaluronidase
what G- bacteria is a late colonizer that interacts with fusobacterium nucleatum and treponema?
P. gingivalis
what bacteria is most commonly associated with endodontic lesions and has the ability to degrade collagen and has proteolytic activity?
Porphyromonas endodontalis
which bacteria is associated with mod-severe gingivitis, necrotizing ulcerative gingivitis, chronic periodontitis, and periodontal abscess?
Prevotella intermedia
T/F Prevotella intermedia does not have an endotoxin
false
is prevotella intermedia an early or late colonizer?
late
which G- bacteria is an early colonizer that interacts with S. oralis, S sanguis, and A israelli?
Prevotella loescheii
what are two important virulence factors for Fusobacterium nucleatum?
endotoxin
succinic acid (inhibits phagocytosis)
what bacteria causes Vincents angina as mixed infection with spirochetes?
fusobacterium nucleatum
what is a big anchor that attaches to both early and late colonizers?
Fusobacterium nucleatum
which virulence factor can inhibit phagocytosis?
succinic acid (fusobacterium)
what are the bacteria that cause chronic adult periodontisis?
poryphoromas gingivalis
fusiform nucleatum
prevotella intermedia
aggregitobacter
campylobacter rectus
tannerella forsythensis
capnocytophaga
treponema
which bacteria can colonize the nose of health individuals?
Neisseria meningitis
what bacteria is responsible for vascular damage and circulatory collapse?
Neisseria meningitis
what are the 3 main symptoms for Neisseria meningtidis?
headache
stiff neck
fever
which G- bacteria is spread by droplet infection and is usually spread among college students?
Neisseria meningitis
which bacteria can you use blood, CS fluid, or skin lesions to diagnose?
Neisseria meningitis
which bacteria infects the genitourinary tract and pharynx?
N. gonorrhea
what bacteria can cause yellow purulent urethral discharge?
N. gonorrhea
which bacteria has an incubation time of 2-8 days and 20-80% of females are asymptomatic?
N. gonorrhea
what are the major complications of N. gonorrhea?
salpingitis
PID
what bacteria can be transmitted through an infected birth canal and cause ophthalmia neonatorum?
N. gonorrhea
what is the 1st and 2nd most common bacterial venereal disease?
1-Chlamydia
2-Gonorrhea
T/F humans and water are the only reservoirs for gonorrhea
false
-humans are the only reservoirs
what is an early colonizer that attaches to S oralis, S sanguis, A. naeslundii, and Fusobacterium
Veillonella
what is considered a benevolent bacteria in relation to dental caries?
Veillonella
What bacteria metabolize lactic acid into weaker acids like acetic acid and propionic?
Veillonella atypica
what G- bacteria is associated with aggressive and chronic periodontitis and subacute endocarditis ?
Aggregatibacter
what bacteria has leukocidin, resistance to complement mediated killing, endotoxin, collagenase?
Aggregatibacter
what bacteria is found at the apical front of subgingival deposit and penetrate underlying soft tissue?
Aggregatibacter
what bacteria is associated with human bite wounds or fist fight injuries?
Eikenella
what bacteria grows under capnophilic conditions and occurs in chronic marginal gingivitis?
Capnocytophaga
what group of bacteria all occur as normal flora of the intestinal tract?
Enterobacteriaceae
what group is responsible for 40% of all nocosomial infections?
Enterobacteriaceae
what is the most common cause of urethrocystitis, chronic bacterial prostatitis, acute pyelonephritis, diarrheal disease?
E. Coli
what is the most common cause of kidney stones?
Proteus
what is the cause of typhoid fever?
S. typhimurium
what is one of the most common bacterial diseases in the US caused by contaminated beef, eggs, chicken?
Salmonellosis enterica
what is a vibio like G- bacteria that is the #1 cause for diarrhea?
Campylobacter jejuni
what is a curved G- bacteria that is associated with subgingival dental plaque and aggressive periodontitis?
Wolinella
which group of bacteria have endoflagella?
spirochetes
what type of microscopy is needed to observe spirochetes?
dark field
phase contrast
which bacteria is associated with fusiform necleatum in cases of NUG and Vincents angina?
Treponema vincentii
what do Treponema bacteria use for motility to invade periodontal tissue?
endoflagella
what is the etiologic agent of syphilis?
Treponema pallidum
which bacteria cannot be grown on lab media but can be grown on rabbit epithelial cells?
Treponema pallidum
in what stage of syphilis do you get a chancre?
primary
T/F the second stage of syphilis involves skin rash
true
what is characteristic of the tertiary stage of syphilis?
gummas
what is the route of transmission for syphilis?
sexual transmission
wasserman antibodies can be used as a seriological test?
Treponema
what bacterial causes legionnaires disease?
Legionella pneumophila
which bacteria has airborne transmission and can be found in air conditioning systems and water systems?
Legionella
what bacteria can be spread to dental personnel from high speed handpiece generated aeurosols?
Legionella
which bacteria are mostly saprophytes in soil and water?
pseudomonas
which bacteria has pyocyanin and pyoverdin(green fluorescent pigment)?
pseudomonas aeruginosa
which bacteria is observed in cases of open root canals?
Pseudomonas aeruginosa
which bacteria can cause swimmers ear, folliculitis(from hot tubs), and ecthyma grangrenosum?
Pseudomonas aeruginosa
which bacteria is an obligate intracellular parasite?
Chlamydia trachomats
which bacteria causes trachoma, inclusion conjuncitivitis, and genital and rectal infections?
Chlamydia
Chlamydia often occurs as a dual infection with?
Gonorrhea
what is the most common sexually transmitted disease?
Chlamydia
which bacteria lacks a true cell wall?
Mycoplasma
what are 2 obligate intracellular bacteria that are commonly transmitted by insects?
Rickettsia
Coxiella
what are all of the late colonizers?(7)
Capnocytophaga sputigena
Aggregatibacter
Prevotella intermedia
Selenomonas flueggei
Porphyromonas gingivalis
Eubacterium
Treponema denticola
What are all the early colonizers?(13)
Caphocytophaga ochracea
C.gingivalis
Actinomyces israeli
Propionibacterium acnes
Prevotella loeschii
P.denticola
S. gordonii
Haemophilus parainfluenza
Actinomyces naeslundii
Veillonella atypica
Eikenella corrodens
S oralis
S mitis
which bacteria has its highest incidence in the US among unimmunized children less than 10 ?
Corynebacterium diptheriae
which bacteria has been used to study bacterial calcification and calculus formation?
Cor. matruchotti
what are the most common anaerobic contaminants of blood cultures?
Propionbacterium
which bacteria requires boiling in an acid alcohol solution with a red dye in order to stain?
Mycobacteria
what are the pulmonary signs of tuberculosis?
progressive cough and mucoid sputum
T/F primary TB shows extensive infiltration in the upper lungs and bronchi
false
-primary: fine areas of infiltration and enlarged lymph nodes
-secondary: extensive infiltration in the upper lungs and bronchii
microscope, PCR, DNA probe, and AFB smear can be used to diagnose which bacteria?
M. tuberculosis
which bacteria do you need to take antibiotics for prolonged periods (18-24 months)?
M. tuberculosis
T/F the tuberculosis vaccine is a capsular polysaccharide
false
-live attenuated
which organisms are contained in granulomatous lesions and within granules in sinus channels?
Actinomyces
which bacteria has a higher infection rate in males and is usually caused by direct introduction into injured tissues?
Actinomyces
T//F actinomyces are early colonizers
true
which bacteria is found in dental plaque but a specific relationship to disease has not been established?
Actinomyces odontolyticus
which bacteria colonize the oral cavity PRIOR to tooth eruption?
A. naeslundii
which bacteria does NOT colonize prior to tooth eruption?
A. viscosus
which bacteria is commonly found in soil and can cause pneumonia in compromised individuals?
Nocardia
which bacteria is a G+ pleomorph isolated on root surface and associated with root caries and endocarditis?
Rothia dentocariosa
the group Clostrida can cause what 4 diseases?
tissue infections
food poisoning
tetanus
botulism
edema fluid, gas accumulation and increased pressure leading to decrease in circulation promotes the spread of what bacteria?
Clostridium perfringens
Match the correct answers:
1. infection of only necrotic tissue
2. invasive infection, foul smelling, massive tissue damage
3. infect tissue that is already dead (remains localized)
A. gas gangrene
B. clostridia cellulitis
C. superficial contamination
1. C
2. A
3. B
the essential factor predisposing you this bacteria is trauma (deep lacerated or crush wound of muscle)?
Clostridium perfringens
what is the treatment for gas gangrene (C. perfringens)?
-remove infected tissue
-hyperbaric oxygen
which bacteria can cause pseudomembrane colitis?
C. difficile
what are the risk factors for C. difficile?
antibiotic exposure
impaired intestinal motility
older age
females
the classic initial signs of infection by this bacteria is muscle stiffness and lockjaw?
Clostridium tetani
which bacterias's toxin is ingested and incubates for 24-36 hours?
C.botulism
for which bacteria could you look for toxin in feces, serum, vomit,or food samples?
C. botulism
what are the 3 black pigmenting bacteria?
Tannerella
Porphyromonas
Prevotella
what are all the G+ bacteria groups for this section?(8)
1. enterococcus
2. lactobacillus
3 .corynebacteria
4. propionbacterium
5. Mycobacteria
6. Actinomyces
7. Clostridia
8. Eubacterium
infection by which bacteria is usually a result of fecal material spilled into peritoneal cavity during surgery cancer etc?
Bacteriodes fragilis
what is the most resistant of all anaerobes to antimicrobial agents?
Bacteroides fragilis
which bacteria is strongly associated with peridontal disease and can cause active tissue and bone destruction?
Tannerella forsythensis
which 2 bacteria are found in subgingival and supragingiva biofilms, tongue, and buccal mucosa and saliva?
Tannerella forsynthesis
P. gingivalis
which bacteria can be reservoirs for recolonization of the gingival crevice after periodontal disease?
Tannerella forsynthesis
P. gingivalis
which bacteria is a strict anaerobe that has the ability to degrade collagen and has proteolytic activity?
Porphyromonas endodontalis
which bacteria colonize the gingival sulcus and have been isolated from periodontal and gingivitis lesions?
Fusobacterium necleatum
which bacteria are divided into serotypes by their capsular polysaccharides?
Neisseria meningitidis
which bacteria when graim stained will show many PMNs with intracellular G- dipplococci with flattened sides?
N. gonorrhea
what are the 3 tests to diagnose N. gonorrhea?
gram stain
ELISA
genetic probe(test of choice)
which bacteria grows on the selective media typtone soy serum bacitracin vancomycin agar?
Aggregatibacter
which bacteria is responsible for 40% of neonatal meningitis?
E. coli
What Enterobacteriaceae are the most frequent causes of UTI?
Proteus
Klebsiella
Enterobacter
what are the clinical manifestations for salmonella?
enteritis
diarrhea
which campylobacter has been associated with gingivitis and localized agressive and chronic periodontitis?
C. rectus
T/F oral spirochetes often occur in gingival margin and in subgingival plaque
true
which disease are 50-70% of infections in women clinically silent?
chlamydia
which bacteria have the lowest GC values?
Mycoplasma
which bacteria is transmitted by infectious droplet and the infectious dose is low (100 cfu)?
Mycoplasma
which bacteria is responsible for 40% of neonatal meningitis?
E. coli
What Enterobacteriaceae are the most frequent causes of UTI?
Proteus
Klebsiella
Enterobacter
what are the clinical manifestations for salmonella?
enteritis
diarrhea
which campylobacter has been associated with gingivitis and localized agressive and chronic periodontitis?
C. rectus
T/F oral spirochetes often occur in gingival margin and in subgingival plaque
true
which disease are 50-70% of infections in women clinically silent?
chlamydia
which bacteria have the lowest GC values?
Mycoplasma
which bacteria is transmitted by infectious droplet and the infectious dose is low (100 cfu)?
Mycoplasma
what is the most common place for infectious agents to gain access into the body?
respiratory tract
what is the normal biota of the upper respiratory tract?
Strep pyogenes
Haemophilus influenza
Strep pneumonia
N. meningitidis
Staph aureus
what is the causative agent of acute otitis media (ear infection)?
Strep pneumonia
Haemophilus influenza
what is the causative agent of pharyngitis?
Strep pyogenes
what is the halmark for C. diptheria?
pseudomembrane
what is the vaccine for diptheria?
DTaP vaccine
what is caused by Bordtella pertussis and is most serious in infants?
Whooping cough
inadequate nutrition, lung damage, poor access to medical care, and lowered immune system are risk factors for?
M. tuberculosis
what are the 3 different types of pneumonia?
Strep pneumonia
Legionella pneumonia
Mycoplasma pneumonia
what is the normal biota for the cardiovascular and lymphatic systems?
NONE
-closed system with no bacteria
what is the causative agent for Anthrax?
Bacillus anthracis
what is the normal biota for the nervous symstem?
no normal biota
what is inflammation of the menginges?
meningitis
which bacteria has petechiae, attaches to the nasopharynx and is detected via CSF gram stain?
N. meningitidis
what is the most frequent cause of neonatal meningitidis, and is transmitted by the mother during passage through the birth canal?
Streptococcus agalactiae
T/F accessory organs of GI tract are full of bacteria
false
-no bacteria
what is a sharp or burning pain emanating from the abdomen and what bacteria causes it?
-gastritis
-Helicobacter pylori
which bacteria produces shiga toxin and can cause hemolytic uremic syndrome?
E coli
what is the most common bacteria cause of diarrhea in the US?
Campylobacter jejuni
what is the cause of pseudomembranous colitis?
Clostridium difficile
what causes rice water stools by an A-B enterotoxin?
Vibrio cholera
what are the 3 food poisonings caused by preformed toxin?
Staph aureus
Bacillus cereus *fried rice
C. perfringens
the vagina has normal biota but above the ___ there is no bacteria?
cervix
which bacterial species is involved in lowering the pH of the vagina?
Lactobacillus
what are 3 signs/symptoms of gonorrhea?
urethitis
salpingitis
pelvic inflammatory disease
what are the 2 diphtheriods in the skin?
Corynebacterium
Propionbacterium acnes
what is caused by microbial digestion of excess sebum and what bacteria is the causative agent?
acne-proprionbacterium acnes
what are the causative agents of impetigo?
S. aureus
Step pyogenes
what is a fast spreading infection of the dermis and subcutaneous tissue and what causes it?
cellulitis
-staph aureus
-strep pyogenes
gas gangrene is most often caused by?
C. perfringens
what are all the cariogenic bacteria?
-Lactobacillus
-Actinomyces naeslundii
-Streptococcus mutans
-Streptococcus sobrinus
what are all the bacteria that cause root caries?
Lactobacillus
Actinomyces naeslundii
-Streptococcus mutans (**causes initial lesion)
Rothia
Bifidobacterium
Proprionibacterium
all of the bacteria that cause chronic adult periodontitis?
-Aggregatibacter
-Capnocytophaga
-Porphyromonas gingivalis
-Fusobacterium nucleatum
-Tanerella
-Campylobacter rectus
-Treponema
-Prevotella intermedia
what are all the bacteria that cause NUG?
-Fusobacterium nucleatum
-Treponema vincentii
-Prevotella intermedia
what are all the bacteria that can cause dentoaveloar abscess?
Fusobacterium nucleatum
P. gingivalis
Prevotella intermedia
causes of Ludwigs angina?
P gingivalis
Prevotella intermedia
T/F Prevotella intermedia is involved in all periodontal and dentoalveolar disases EXCEPT aggressive periodontitis
true
what bacteria are the pioneers for plaque?
Strep gordonii, oralis, mitis and sanguis
what are all the causes of aggressive periodontitis?
Aggregatibacter
Capnocytophaga
Porphyromonas gingivalis
Fusobacterium nucleatum
Eikenella corrodens
Wolinella recta
what are the bacteria in the stomach?
Lactobacillus
Helicobacter
what are the bacteria in the small intestine?
lactobacillus
bacteroides
fusobacterium
staph
strep
enterococcus
what are the bacteria found in the colon?
bacteroides
bifidobacterium
clostridium
fusobacterium
entercoccus
enterobacteriaceae
what bacteria is found in open root canals?
pseudomonas
Pits and fissures of occlusal surfaces are dominated by?
streptococci
histatins, statherins, lysozyme, proline rich protein, carbonic anhydrase, mucin 1 and 2, slgA are components of ?
saliva
what plays a primary role in the formation of the acquired pellicle and helps prevent acid attack of enamel?
glycoproteins (mucin)
what are the nonspecific defense factors of saliva?
lysozyme, lactoferrin, and histatins
what is a serum like fluid that flows through the junctional epithelium of the gingivae?
gingival crevice fluid
what is the mean pH for saliva?
6.75-7.25
T/F the persistence and diversity of the resident oral microflora is due to the diet rather than the endogenous nutrients provided by the host
false
-the opposite is true
what is in toothpaste that gets incoportated into the enamel and has lasting effects?
fluoride
what is one of the active agents in mouthwash?
chlorohexidine
what are the 5 host defenses against oral bacteria?
-physical removal by saliva
-lysozymes hydrolyze peptidoglycan
-lactoferrin sequesters iron
-sialoperoxidase inhibts glycolysis
-histatins are bacteriostatic/bacteriocidal
what is the establishment of a resident oral microflora derived from mom and caregivers?
acquisition
the first colonizers of the mouth are the ____ microbial community?
pioneer
what is the predominant organism in the mouth?
streptococci
what is the increase in the number of anaerobes due to the aerobic and faculative pioneers lowering the redox potential?
autogenic succession
the bacterial components which function in adherence are ___ and the host derived factors are ___
adhesins
ligands
what bacteria use the galactosyl ligand?
Actinomyces naeslundii
Fusobacterium nucleatum
Prevotella intermedia
what is a stable situation with a high species diversity (oral microflora)?
climax community
collagen fibers act as receptors for?
Porphyromonas gingivalis
what are 3 bacterial adhesins?
fimbriae
fibrils
surface active compounds
T/F Brauns lipoprotein is linked to peptide glycan
TRUE
what are the human infections caused by biofilms?
endocarditis, otitis media, prostatitis, cystic fibrosis, periodontitis, dental caries
what is cell to cell communication where bacterial population density determines the expression of specific genes?
quorum sensing
what is the model for biofilm development?
Pseduomonas aeruginosa
major pathogens in dental equipment are?
Pseudomonas aeruginosa
legionella pneumophila (obligate intracellular parasite for eukaryotes)
mycobacteria
acanthamoeba (protozoan)
how many bacterial species does dental plaque contain?
500
plaque that becomes calcified is called?
tartar
what are the pioneer species for enamel surfaces?
strep
actinomyces naeslundii
A. israelii
Capnocytophagg gingivalis
what are the colonizers of root surfaces?
streptococci
Propionbacterium
Rothia dentocariosa
what stage of biofilm development involves bacterial adhesins and cryptitopes?
stage 3
which bacteria possess galactosyl binding lectins?
A. viscosus
F. nucleatum
P. intermedia
what stage has co-aggregation and formation of corn cob structures?
stage 4
what are some antagonistic microbial interactions in dental plaque?
bacteriocins
hydrogen peroxide
organic acids
low pH
nutrient competition
what is the localized destruction of the tissues of the tooth by bacterial action?
dental caries
what is one of the most common human diseases?
dental caries
caries of the enamel are common up to age ____
20
root surface caries are more common in _____
older adults
what theory proposes that only a limited number of bacterial specie are involved in oral disease?
specific plaque theory
what theory proposes that any microorganism can play a role in oral disease?
non specific plaque hypothesis
what are the cariogenic bacteria?
strep mutans
strep sobrinus
actinomyces naeslundii
lactobacillus
what is the primary influence for human enamel caries?
GTF glucosyltransferase
what is the substrate for GTF and what does it synthesis?
substrate-sucrose
synthesis glucan
T/F in loose glucan rich biofilms the sugar molecules penetrate deep and acid is formed near the surface of the tooth
true
root surface caries occur in what age group?
middle aged and older adults
what affect does pH have on fluoride saturation?
at low pH fluoride doesnt saturate as well
necrosis of the root canal and destruction around the apex of the root is a consequence of?
root surface caries
what is the critical pH for enamel demineralization?
5.5
what effects the gingiva, gingival attachement, periodontal ligament, cementum and supporting aveolar bone?
periodontal disease
what disease does the gingival crevice become a pocket, EH decreases and GCF increases?
periodontal disease
what bacteria may actively invade gingival tissues?
treponema
prevotella intermedia
what bacteria can be distinguished from streptococci from a bile esculin test?
Entercoccus
what bacteria is part of the normal flora of the mouth, GI tract, and female genital tract?
Lactobacillus
Lactobacillus produces _____ from sucrose?
lactic acid
data indicates that the extent of dental caries could be correlated with the number of _____ and _____ present?
S. mutans
Lactobacillus
penicillin with an aminoglycoside can be used to treat?
lactobacillus
what can be treated with benzoyl peroxide, eythromycin and clindamycin?
propionbacteria
what can be diagnosed with chest X ray and AFB test from sputum?
M. tuberculosis
what can be treated with isoniazid, rifampin and streptomycin?
M.tuberculosis
what can be treated by debridement of wound and large doses of antibiotics perhaps barbiturates or a curare like agent to control spasms?
C. tetani
what can be treated with excision of necrotic tissue and metronidazole and clindamycin?
C. perfringens
is P. gingivalis an early or late colonizer?
late
the major site of colonization for ____ is gingival sulcus, it has also been isolated from periodontal and gingivitis lesions and from upper respiratory tract.
Fusobacterium nucleatum
what virulence factor is responsible for vascular damage and circulatory collapse caused by meningitis?
LPS
what are the clinical manifestations of N. meningitidis?
fulminating meningococcemia
meningitis
T/F meningitis is spread via droplet infection involving close contact
true
which bacteria reduces demineralization of enamel?
Veillonella atypica
what is G- bacillus that is capnophilic and is associated with localized aggressive and chronic periodontitis?
Eikenella
what is an opportunistic pathogen that has been associated with gingivitis, localized agressive periodontitis, and other systemic infections?
Capnocytophaga
what are the 3 oral spirochetes?
T. denticola
T. vincentii
T.orale
what is the treatment for treponema?
metronidazole
chlorhexidine
penicillin
what bacteria can be diagnosed by measuring wassermann antibodies?
treponema pallidum
what is the normal biota of respiratory tract?
strep pyogenes
strep pneumonia
staph aureus
haemophilus influenza
N. meningitidis
the signs of ____ include headache, painful or stiff neck, and fever (increased white blood cells in CS fluid)
Meningitis
what is the normal flora of the skin?
staph aureus, epidermitis
strep
corynbacterium
propionbacterium
micrococcus
the mouth is lined with what types of epithelium?
stratified squamous
what is the oral habit with keratinized and non keratinized stratified squamous epithelium?
buccal mucosa
T/F the gingiva, hard palate,and anterior dorsal aspect of the tongue have non keratinized cells
false
-keratinized
the dorsum of the tongue has many or few bacteria?
many, highly colonized
what aspect of the tongue makes it highly colonized by anaerobic bacteria?
low redox potential
what is the only site in the body that has hard non shedding surface for microbial colonization?
teeth
the gingival crevice is bathed in ?
gingival crevice fluid
T/F areas between adjacent teeth and the gingival crevice offer no protection from the environment
false
-protect from adverse conditions
T/F smooth surfaces are more exposed to environmental effects
true
what tooth surface is dominated by streptococci and have the highest incidence of caries?
pits and fissures of occlusal surface
what is a complex mixture of inorganic ions and proteins?
saliva
what component of saliva is composed of carbs and absorbs tightly to the surface of teeth becoming part of enamel pellicle?
mucin
what component of saliva acts as the primary source of nutrients for microflora?
glycoproteins (mucin)
T/F metronidazole and clindamycin are antibiotics for Bacteriodes fragilis
true
T/F flow rate of saliva is slowest in lower anterior lingual and fastest in upper anterior buccal
false
-opposite is true
what is the flow rate for health y and diseased states for GCF?
-healthy=slow
-diseased=increased rate
what are all the factors affecting growth of bacteria in oral cavity?(5)
temperature
oxidation reduction potential
pH
nutrients
adherence
what is the main source of endogenous nutrients in the mouth?
saliva
what type of succession involves non microbial influences which alter pattern of community development?
allogenic
what type of succession involves the fact that aerobic/facultative pioneer species lower redox potential?
autogenic
after tooth eruption in children the frequency of what 2 groups of bacteria increase?
spirochetes
black pigmenting bacteria
what is usually less that 1 u thick and is composed of acidic proline rich proteins and statherin?
acquired enamel pellicle
what are rod shaped protein structures that aid in adhesion and are thinner and shorter?
fibrils
what is formed by the selective absorption of components from primary saliva and to a lesser extent gingival crevice fluid to surfaces of oral cavity?
pellicles
what are high MW surface active polymers with one hydrophic end and give examples?
Amphiphilic polymer
-Lipopolysaccharides
lipoteichoic acid
lipoproteins
what are high MW surface active polymer with hydrophobic groups across the entire molecule and give examples?
polyphilic polymer
-hydrophobic polysaccharide
what is the term for molecules left after deadhesion>
desorption footprints
the development of a _____ depends on the interactions between adhesins on the microbial cell surface and ligands on host surface
climax community
large accumulations of microorganisms on teeth is called?
dental plaque
what is the main beneficial functions of forming a climax community?
prevent colonization by exogenous organisms (may be pathogenic)
what is a structured community of bacterial cells enclosed in self produced polymeric matrix and adhered to a surface?
biofilm
what are the 3 essential components of biofilm?
bacteria
glycocalyx
surface
human infections involving biofilms?
endocarditis
otitis media
prostatitis
cystic fibrosis
periodontitis
dental caries
biofilms are hetero or homogenous monolayers?
heterogenous
what are the signal molecules for G+ vs G-?
G+ = small peptides
G- = homoserine lactone
advantages of biofilms for bacteria?
anchor, traps nutrients, resistance to antimicrobial agents, protected from antibodies, sweeping, drying and predators
genes responsible for biofilm development?
las gene system
algC gene
what are the major opportunistic pathogens found in dental unit water lines?
Pseudomonas aeruginosa
Legionella pneumophila
mycobacteria
acanthoamoeba
T/F the majority of plaque is found in fissures and the gingival crevice
true
how many bacteria are in saliva?
10^8 / mL
what are the pioneer species (within 2 hours of cleaning)?
mitis streptococci
actinomyces naeslundii and israeli
Capnocytophagg gingivalis
what are the bacteria commonly found on root surfaces?
mitis streptococci
Propionbacterium
rothia dentocariosa
T/F each microhabitat potentially could support the growth of different microbial communities
true
genes responsible for biofilm development?
las gene system
algC gene
what are the survival factors of many of the pioneer species?
IgA protease
neuraminidase
what are the major opportunistic pathogens found in dental unit water lines?
Pseudomonas aeruginosa
Legionella pneumophila
mycobacteria
acanthamoeba
calculus includes what minerals?
apatite
brushite
whitlockite
T/F the majority of plaque is found in fissures and the gingival crevice
true
T/F only 10% of adults have calculus
false -80%
how many bacteria are in saliva?
10^8 / mL
what are the pioneer species (within 2 hours of cleaning)?
mitis streptococci
actinomyces naeslundii and israeli
Capnocytophagg gingivalis
what are the bacteria commonly found on root surfaces?
mitis streptococci
Propionbacterium
rothia dentocariosa
T/F each microhabitat of a biofilm potentially could support the growth of different microbial communities
true
what are the survival factors of many of the pioneer species?
IgA protease
neuraminidase
calculus includes what minerals?
apatite
brushite
whitlockite
T/F only 10% of adults have calculus
false -80%
what are the beneficial factors of microbial interactions?
enzyme complementation
food webs
coaggregation
what involves the dissolution of the enamel and the transport of calcium and phosphate ions away into the surrounding environment
lesion formation
what are caries primarly on proximal tooth surfaces classified as?
smooth surface caries
what is the primary lesion of caries?
white spot lesion
how do you diagnose caries?
direct observation
probing
radiographs
experimental methods
microbiological test
what are the 4 main cariogenic bacteria?
streptococcus mutans
streptococcus sobrinus
actinomyces naeslundii
lactobacillus
what allows for both adhesion to tooth surface and cohesion within biofilms?
GTF
what promotes stacking of chains via H bonds?
glucan chains
T/F glucose which is the only substrate for GTF is also a major factor in pathogenesis of dental caries
false
-sucrose
T/F cariogenic biofilms contain less glucans
false
-large amounts
what exposes the cementum of the root to microbes (happens in old age)?
gingival recession
what property of cariogenic bacteria allows for acid production to continue in the absence of dietary sugars?
intracellular polysaccharide production
-storage granules that store sucrose
during patient evaluation what needs to be assessed?
dietary habits
salivary flow rate
microbiological analysis
what is the CFU for high caries risk?
> 10^6 S. mutans or 10^4 lactobacillus
what CFU is low caries risk
<10^4 S. mutans or 10^3 lactobacillus
sealants are less effective for ____ because matrix can supply nutrients.
3rd degree caries
what is the effective agent for controlling plaque in mouthwash that disrupts the cell membrane?
chlorohexidine
what are the 2 most common periodontal diseases?
gingivits
periodontitis
what is characterized clinically by gingival changes in color, form, position and surface appearance?
gingivitis
what is the difference of the gingival crevice to other parts of the mouth?
more anaerobic
bathed in gingival crevice fluid
which area of the mouth has little sIgA (antibodies)?
subgingival area
what is produced by many G- bacteria in periodontal pockets and have an unpleasant odor and is a major constiutuent of halitosis?
VSCs (volatile sulfur containing compound)
what is a nonspecific inflammatory response to dental plaque involving the gingival margins and can happen to everyone with poor oral hygiene?
chronic marginal gingivitis
what is the most common form of advanced periodontal disease affecting general population (70-80%)?
chronic adult periodontitis
what disease involves the gingiva and has loss of attachement between the root surface and alveolar bone and bone loss?
chronic adult periodontitis
what factors predispose to chronic adult periodontitis?
subgingival calculus
overhang restorations
crowded teeth
which disease has irregular alveolar bone loss around teeth, gingival recession and halitosis and offensive tase?
chronic adult periodontitis
what causes cells to release metalloproteins?
cytokines
lysosomal enzymes
what are all the bacteria that cause chronic adult periodontis?
capnocytophaga
aggregatibacter
prevotella intermedia
p. gingivalis
fusobacterium nucleatum
campylobacter rectus
tannerella forsythensis
treponema
what is the treatment for chronic adult periodontitis?
removal of plaque and endotoxin
-may need surgical access
-antibiotics
what disease is observed in teenagers and manifests as severe marginal inflammation with rapid loss of bony support?
agressive periodontitis
which oral disease does the patient have adequate oral hygiene and depressed neutrophil chemotaxis?
agressive periodontits
what is the treatment for agressive perio?
tetracycline and mechanical therapy
what are the bacteria involved in aggressive periodontitis?
wolinella
eikenella
fusobacterium nucleatum
P.gingivalis
capnocytophaga
aggregatibacter
what is a painful acute condition in which a gray pseudomembrane is formed on gingiva?
Necrotizing ulcerative gingivitis NUG
T/F NUG patients have an unpleasant metallic taste in their mouth
true
what bacteria causes NUG?
Fusobacterium nucleatum
Treponema vincentii
prevotella intermedia
ultrasonic scaling, chlorohexidine mouthwash and metronidazole is treatement for?
NUG
what is rapid acute inflammation involving the whole pulp or chronic localized abscess?
dentoalveolar abscess
what bacteria cause dentoalveolar abscess?
P gingivalis
fusobacterium nucleatum
prevotella intermedia
what is a bilateral infection of sublingual and submandibular space that causes tissues in the front of the neck to swell and can obstruct airway?
Ludwigs angina
what bacteria cause ludwigs angina?
P. gingivalis
prevotella intermedia
what is destruction of the periodontium resulting ins pus in the gingival sulcus caused by trauma or food impaction?
periodontal abscess
what bacteria cause periodontal absces?
P. gingivalis
prevotella intermedia
fusobacterium nucleatum
treponema
capnocytophagg
actinomyces
what treatment can involve tooth extraction or irrigation with sodium chloride solution?
periodontal abscess
what bacteria group is G-, obligate anaerobes, non motile, nonspore forming rods involved in periodontal disease and endodontic infections?
black pigmenting bacteria
T/F bacteriodes fragilis is an exogenous infection
false
-endogenous
infection by this bacteria resembles other anaerobic infections with foul odor of discharge, tissue necrosis, and gas?
Bacteriodes fragilis
the antibiotics for bacteriodes fragilis is ____ and ____. most strains produce ____(antibiotic resistance)
metronidazole
clindamycin
-make beta lactamase
what is a G- obligate anaerobe that is in high numbers in the gingival crevice and is strongly associated with periodontal disease (chronic, aggressive and abscesses)
tannerella forsynthesis
T/F tannerella forsynthesis is only found in subgingival biofilms
false
-in subgingival and supragingival
what porphyromonas bacteria has the highest numbers in the gingival crevice?
p. gingivalis
what is a late colonizer in plaque that interacts with fusobacterium nucleatum?
prevotella intermedia
which bacteria is a G- thin bacillis with pointed ends?
fusobacterium nucleatum
which bacteria plays an important role in maintaining the integrity of plaque?
Fusobacterium nucleatum
which bacteria causes endogenous pleuropulmonary infections, ANUG, NUP, gingivits, periodontitis, and vincents angina?
fusobacterium nucleatum
what is the treatment for fusobacterium?
metronidazole
clindamycin
which bacteria do not possess flagella but have fimbriae and capsules?
Neisseria
what are the primary antigens for dividing neisseria into serotypes?
K antigen (capsular polysaccharide)
what is the #1 cause of meningitis among individuals 5-29?
N. meningitidis
what is a G- diplococci that can be readily observed along with PMNs?
N. meningitidis
complications of what disease can cause urethral strictures, epididymitis, and prostatitis?
gonorrhea
when gram staining this bacteria you will see many PMNs with intracellular G- diplococci with flattened sides
N. gonorrhea
which Veillonella has been associated with gingivitis?
Veillonella parvula
T/F Aggregatibacter is extremely motile
false
-nonmotile
what are the colonization factors for Aggregatibacter?
fimbriae
capsule
which enterobacteriaceae is not part of the coliform group?
proteus
H,K,O antigens are used to distinguish serotypes for what groups of G- bacteria?
Enterobacteriaceae
which group of bacteria are all involved in acute diarrheal disease?
Enterbacteriaceae
what are the 3 virulence factors for enterobacteriaceae?
endotoxin
antibiotic resistance
urease
what are curved or helical G- motile rods that occur in the gingival sulcus and causes aggressive periodonitits?
Wolinella
what are helical bacteria with a central protoplasmic cylinder surrounded by a ctyoplasmic membrane
spirochetes
seriological test for what bacteria include VDRL, rapid plasma reaginic test and wassermann?
nontreponemal
what tests are used to confirm nontreponemal like FTA-ABS?
treponemal tes(can be used for Treponema pallidum)
what is the common source of tranmission for Legionella?
airborne
T/F legionella does not transmit person to person
true
which bacteria has a capsule like envelope or slime layer and able to use 80 different organic compounds for growth?
Pseudomonas aeruginosa
which bacteria is able to resist high concentration of salt, dyes, and disinfectants?
pseudomonas aeruginosa
what is the cause of folliculitis (from hot tubs)?
pseudomonas aeruginosa
which bacteria causes urethritis(NGU), epididymitis, and procitis?
chlamydia trachomatis
nasopharyngeal infection by this bacteria occurs during pasage through an infected cervix (neonatal pneumonia)?
chlamydia trachomatis
which bacteria lack a true cell wall and gram stain poorly or not at all?
mycoplasma
which bacteria cause atypical pneumonia with subclinical infection, bronchopneumonia, remittent fever, cough and headache for weeks?
mycoplasma pneumoniae
what is responsible for 20% of pneumonia cases and has a low infectious dose (100 cfu)?
mycoplasma pneumoniae
what bacteria has an A-B enterotoxin called cholera toxin?
vibrio cholerae
what are the normal biota for the outer region of the urethra?
strep
staph
corynebacteria
lactobacillus
what are 3 types of UTIs?
bladder (cystitis)
kidneys (pyelonephritis)
urethra (uretheritis)
what are the main causative agents of UTIs?
E coli
Staph saprophyticus
Proteus mirabilis
candida albicans, gardnerella, trichomonas vaginalis cause what?
vaginitis
vaginosis
which normal biota of the skin(staph) is coagulase negative?
staph epidermidis
how many bacteria are in the small intestine?
10^3/mL
prosthodontic and orthodontic appliances can act as?
inanimate reservoirs for bacteria and yeast
what is the specific defense factor in saliva?
immunoglobins (slgA)
T/F the normal flora of the mouth has high numbers of obligate aerobes
false
what are the main exogenous nutrients for oral bacteria?
fermentable carbs
what is an example of a polymorphic polymer?
hydrophobic polysaccharide
ex. capsular polysaccharide
what are examples of excreted SACs?
fatty acids
lipids
amphiphilic and polyphilic polymers
what in the biofilm can act as an anchor, trap nutrients and protect?
glycocalyx
what are the major opportunistic bacteria in dental equipment?
pseudomonas aeruginosa
legionella pneumophila
mycobacterium (nontuberculosis)
acanthamoeba
fluoride can change the metabolism of plaque by?
interfering with bacterial membrane permeability
-reducing glycolysis
-inactivating metabolic enzymes
-inhibiting synthesis of intracellular polysaccharides
what is produced by several of the periopathogenic bacteria particulary G- in periodontal pockets?
VSCs(volatile sulfur containing compounds)
what is a nonspecific inflammatory response to dental plaque?
chronic marginal gingivitis
in contrast to chronic periodontitis, little plaque or calculus is present in periodontal pocket for this disease?
aggressive periodontitis
what is associated with poor oral hygiene, malnutrition, and perhaps systemic disease?
NUG-necrotizing ulcerative gingitivits