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15 Cards in this Set
- Front
- Back
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Carbohydrate Fermentation Test
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One sugar in broth medium
indicator-Phenol Red red=neutral; yellow=acid prod. Durham tube traps gas prod. |
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Gelatin in nutrient broth
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Test indicates the ability of organism to break down gelatin. If organism has enzyme gelitinase, it breaks down and if not, it remains a solid.
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Starch Agar
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Test indicates organism's ability to break down starch via exoenzyme amylase. Iodine added to agar plate to stain.
Clear area around bacteria=positive result |
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Milk Agar
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Test indicates microorganism's ability to break down casein via protease exoenz.
Clear space around bacteria=positive |
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DNA Agar
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Test ind. organisms ability to break down DNA via DNase. 1.0M HCl added to agar plate after incub.
Clear space around bac.=pos |
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Simmons Citrate
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Citrate=only C compound in medium. Use of citrate as C source changes color from green to blue. Uses C, releases ammonium, and changes pH to alkaline.
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Urea Broth
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Test ind. presence of urease. Add phenol red and in presence of ammonia, turns hot pink.
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Catalase
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Test ind. presence of catalase-if present, bubbles form, proving it's aerobic
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SIM-Sulfide Indole Motility Medium
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H2S produces black precipitate
Indole indicated by cherry-red color after adding Kovac's reagent. Motility found by stab line. |
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Mixed Acid Fermentation (MR-VP)
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Mixed-acid prod. ind. by broth turning red after Methyl Red added. Indicated fermentation of glucose.
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2,3-Butanediol Fermentation (MR-VP)
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Broth turns red after alphanaphthol and KOH added if glucose fermented.
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Phenylalanine Agar
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Slant turns green after adding 10% ferric acid if bacteria contains phenylalanine deaminase.
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Tryptone Broth
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Broth changes from yellow to red after 10 drops Kovac's reagent is added if bacteria contains tryptophanase
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O/F Glucose Nutrient Agar
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2 tubes inoculated with same bacteria, 1 sealed by adding sterile mineral oil.
Change from green to yellow in tube w/o oil=oxidative metabolism, change in both tubes from green to yellow=fermentative metabolism, no change=can't use glucose |
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Litmus milk
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Change from blue to purple=alkaline reaction.
Change from blue to pink=acid reaction. Change from blue to white=Litmus reaction Clear brown translucent layer at top=peptonization. |
