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129 Cards in this Set
- Front
- Back
A positive catalase test indicates which Gram positive cocci?
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Stapylococcus
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You have a positive catlase test indicating Stapylococcus so the next test you run is__________, which uses ___________ and a positive result is indicated by _________
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Coagulase
Rabbit plasma clotting of the plasma |
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A positive coagulase test indicates ___________________ and 3 types of this are:
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pathogenic staphylococcus
~Staph. aureus ~Staph intermedius ~Staph hyicus |
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A negative coagulase test indicates ______ and 2 types are ________
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non-pathogenic
~Staph. epidermis ~micrococcus |
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Name the 3 types of pathogenic Staph:
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Staph aureus
Staph. intermedius Staph hyicus |
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True or false
Most bacteria are facultative anaerobes |
true
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Name 2 types of non-pathogenic Staph:
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Staph. epidermis
Micrococcus |
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You have a negative catalase test, which indicates what type of GPCOC?
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Streptococcus
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When you have identified that you have a type of Streptococcus after doing a catalase test, what is typically your next step?
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Inoculate a BAP to test for type of hemolysis
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With Streptococcus, Beta hemolysis is indicative of ___________ and the 6 types are:
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Pathogenic bacteria
~Strep. pyogenes (Lancefield group A) ~Strep agalactiae (Lancefield group B) ~Strep. dysgalactiae (variable hemolysis) ~Strep equi (Lancefield group C) ~Strep zooepidemicus ~Strep canis |
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With Streptococcus, Alpha hemolysis is indicative of what 4 types of bacteria?
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~Strep. pneumoniae
~Strep. suis ~Strep bovis ~Strep equinus |
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With Streptococcus, Gamma Hemolysis is indicative of what 2 types of bacteria?
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~Strep. faecalis
~Strep. uberis |
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What are 3 shapes of GPRODS?
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Small rods
Large rods coccobaccillus |
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To ID GPRODs, one type of test used is the H2S test which uses a _______ and a positive test result is _________
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KIA agar slant
black pigment along stab line |
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To ID GPRODs, one type of test used is the Motility test which uses MIO motility media and a positive test result is indicated by ___________
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media being cloudy throughout
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To ID GPRODs, another test used for ID is the Urease test on a Urea agar slant and a positive result is indicated by ______ and a delayed positive is indicated by ______________
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hot pink agar
pale pink |
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In addition to the Catalase, H2S, Motility and Urease tests, what are 2 other things that can help ID Genus and Species of bacteria being tested?
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Origin of sample
colony morphology |
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Streptococcus grows in _________ and is a __________ bacteria found in MM of ____________.
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twisted chains and pairs
normal flora genital, GI and respiratory tracts |
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Streptococcus is a normal flora ___________bacteria that can be ___________ and causes ______ infections
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facultative
pathogenic pyogenic |
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Streptococcus pyogenes causes _____, belongs to Lancefield group _______ and is __________hemolytic.
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strep throat
A Beta |
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Strep agalactiae Group B is confirmed using what test?
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The CAMP test
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The CAMP test involves the unknown Beta Strep being streaked on a BAP plate perpendicular to Staph aureus. A positive result is indicated by _________
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beta hemolysis around both streak lines with ARROWS at the juncture of the 2 streak lines
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Strep agalactiae belongs to Lancefield group_______ , is a ______ hemolytic and is a leading cause of ___________ and women are NF cervical carriers.
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B
Beta Bovine mastitis |
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Strep equi belongs to Lancefield group _____, is a _______hemolytic and is what causes ___________, a very contagious _____ that involves enlarged lymph nodes which leads to dyspnea. A Bacterin is used __________
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C
Beta Equine Strangles URI only during outbreaks |
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Strep. dysgalactiae belongs to Lancefield group ____, is a _____ hemolytic and is a minor cause of________
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C
Beta bovine mastitis |
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Strep equi subspecies zooepidemicus belongs to Lancefield group ____ and is a ________ hemolytic that affects a wide variety of animals and is seen in ____________.
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C
Beta wound & genital infections, mastitis |
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Strep equi subspecies zooepidemicus is becoming more common in shelter dogs and causes ___________
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acute canine respiratory disease with cough, fever and lung hemorrhage
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Strep canis is a ___________ hemolytic and is seen in _________
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Beta
UG infections and cystitis skin, wound and ear infections |
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The most numerous bacteria in oral cavity are:
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Streptococcus Alpha hemolytic bacteria
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Strep. pneumoniae is an ___________ hemolytic without a Lancefield group classification. This is a leading cause of _____________in ____________
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Alpha
primates (Pneumococcal variety), humans (Bacterial) guinea pigs rarely causes it in dogs |
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Strep pneumoniae is __________ resistant, has an ________course and mortality is __________
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very antibiotic
acute high |
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Strep suis is an __________ hemolytic that is __________for young pigs that causes meningitis, septicemia and arthritis.
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Alpha
pathogenic |
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Enterococcus faecalis is a ________ hemolytic and a _________that is important for people because it is a leading cause of _______ but is also a common in _______ and tends to be antibiotic resistant.
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Gamma
NF bacteria in the GI tract nosocomial infections opportunistic infections |
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Staphylococcus tends to be antibiotic resistant due to the actions of this enzyme _________________
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Beta lactamase
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Beta lactamase is an enzyme that destroys penicillin like antibiotics but we have _____________
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drugs that inactivate this enzyme
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Staphylococcus are bacteria that grow in __________,and are ____________ that are resistant to drying, disinfectants and antibiotics.
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clusters
facultative anaerobes AND Aerobes |
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Staphylococcus are a NF species but become __________ causing _______ due to an enzyme that facilitates attachment to skin and MM's (tissue invasion.
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pathogenic
pyogenic infections |
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Name the 3 Staphylococcus Coagulase positive bacteria
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Staphylococcus aureus
Staphylococcus intermedius Staphylococcus hyicus |
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Staph aureus,is a ________ hemolytic that causes a ______Coagulase test and is _________ for humans and many animals.
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Beta (double zone)
positive pathogenic |
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Staph aureus causes a wide variety of infections and is a leading cause of _____________. Commonly caused infections include _______________ as well as food poisoning.
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bovine mastitis
surgical, wound and skin infections GU infections and Osteomyelitis in open fractures |
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Staph. intermedius is a newer species similar to Staph aureus and is the most common species of staph from _____, is a __________ hemolytic.
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dogs
beta (Double zone) |
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Staph intermedius is a common cause of __________
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mastitis, would, skin and surgical site infections, GU tract, arthritis and bacteremia
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MRSA stand for
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Methicillin
Resistant Staphlococcus Aureus |
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MRSI stands for
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Methicillin
Resistant Staphylococcus Intermedius |
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Stapholococcus hyicus is a catalase and coagulase positive bacteria that is usually _______ hemolytic and is known for causing ___________ pig disease
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Gamma
greasy |
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Greasy pig disease is also called __________ and is more common in __________ and is highly contagious skin disease that involves yellowish crusts on snout, ears and head.
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Exudative Epidermitis
piglets |
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Name the 2 coagulase negative Staphylococcus non-pathogenic species
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Staph. epidermis- NF on skin but can cause opportunistic infections
Micrococcus |
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Two types of anaerobic GPCOC are
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peptococcus
peptostreptococcus **are NF of alimentary tract, important in mixed infections, abscesses and effusions |
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GPRODS have 3 types that this class is studying. What are they?
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Small rods
Large rods branching--a higher evolutionary form that are fungi like, branching and filimentous |
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Small GPRODS are more important in _________
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large animal
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What are the 5 Genus classifications for SMALL GPRODS?
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Corynebacterium
Listeria Erysipelothrix Arcanobacterium Rodococcus |
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What are the 2 Genus classifications for Large GPRODS?
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Bacillus (aerobic)
Clostridium (anaerobic) |
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Corynebacterium pseudotuberculosis, a small GPROD, is also know as what in sheep ______ and in horses it is known as _______
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CLA- Caseous Lymphadenitis in sheep
Pigeon fever in horses -Ulcerative lymphangitis |
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Corynebacterium pseudotuberculosis is acquired by sheep through
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wounds from shearing, castration, dehorning and tail docking
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Corynebacterium pseudotuberculosis is the club shaped rods bacteria that are also known for looking like ______
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chinese letters
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Corynebacterium pseudotuberculosis is acquired by horses and mules through ___________
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puncture wounds
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Corynebacterium pseudotuberculosis pathology involves the bacteria spreading via the ______.
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lymphatics
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Corynebacterium pseudotuberculosis in sheep cause the lymph nodes to become huge and then _________ and possibly ulcerate.
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abscess w/caseous pus
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Corynebacterium pseudotuberculosis in horses spreads to lymph nodes but can cause ________ as well as lameness and the characteristic _________
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abscess anywhere
lameness edema in the pectoral muscles |
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Treatment for Corynebacterium pseudotuberculosis is difficult, especially in herds. ____________ abscesses make treatment difficult and __________ is often required and isolation is recommended due to the pus being highly infectious. Herd culling is often needed.
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Walled off
surgical excision with a suture drain |
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Prevention of Corynebacterium pseudotuberculosis involves immunization with _______ that is used _________ and control of flies is important due to their being a mechanical vector.
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Bactrin
only during outbreaks |
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Corynebacterium renale have _______ that faciliatate attachment to the MM of ___________
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pili
GU tract |
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Corynebacterium renale cuases disease in ___________
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cattle and sheep
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In cattle, Corynebacterium renale causes ______________
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bovine pyelonephritis, cystitis
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In sheep, Corynebacterium renale causes
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bacterial balanopothitis--> "pizzle rot"
and vulvulitis |
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Corynebacterium renale causes pyelonephritis and cystitis which involves an ______________ and often involves _______ which leads to the characteristic bloody purulent urine.
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ascending pyogenic infection of urinary tract
hemorrhagic necrosis and ulceration of MM lining urinary tract |
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Corynebacterium renale is seen more often in sheep on high protein diets which _________, which leads to urine__________
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alters urine ph
scalding of skin and MM of external genitalia, which predisposes skin to infection |
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Listeria monocytogenes is a small GPROD that is motile, found in feces, soil, water, milk, straw and silage that is a common cause of ___________, has one form in people and _____ forms in animals.
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food
2 |
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In people, Listeria monocytogenes takes one form known as ________, which is a cause of food poisoning with V/D as well as meningitis in ________
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Listeriosis
immunocompromised people |
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In animals, Listeria monocytogenes takes 2 forms. What are they?
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Visceral form and Neural form
|
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In Listeria monocytogenes, the Visceral form is more common in ______ and causes widely disseminated ____________ on internal organs.
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monogastric animals, though rare in dogs, common in rabbits, chinchillas, GP
abscesses |
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In Listeria monocytogenes, the Neural form is more common in _________ and is commonly called "________" due to its affects on the _______ and is usually fatal.
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ruminants, esp. cattle and sheep
circling disease CNS with unilateral ataxia |
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Erysipelothrix rhusiopathie, a small GPROD, is resistant to drying and can survive for long periods in water, soil and food. The reservoir for this bacteria is ____________
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in the NF of the throat, mouth of swine and fish slime
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Erysipelothrix rhusiopathie is pathogenic for ____________
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pigs, birds (esp. turkeys) fish, marine mammals and people
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Erysipelothrix rhusiopathie in people causes what in people?
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Fish Handlers Disease
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Erysipelothrix rhusiopathie in pigs causes what disease and what age is most susceptible?
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Swine erysipelas
3-18 month old piglets are most susceptible |
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The Swine erysipelas form of Erysipelothrix rhusiopathie has 4 forms. What are they?
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~Skin form -most common
~Arthritic - older pigs ~Acute - septicemia, high fever, typically fatal ~Cardiac-endocarditis, usually fatal **immunization available |
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Erysipelothrix rhusiopathie in the skin form of Swine erysipelas typically looks like ______, where it gets its name ____________
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red to purple skin patches in a diamond/ square shape
"Diamond Skin Disease" |
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Erysipelothrix rhusiopathie in marine mammals and fish typically is seen with ____________
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skin lesions and septicemia
|
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Arcaneobacterium pyogenes is a small GPROD that affects ruminants and gains entrance through ______ and causes ___________ in wounds, surgical infections, mastitis, pneumonia and umbilical infections.
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wounds
pyogenic infections |
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Rhodococcus equi is a small GPROD that causes ________ in foals, who are most susceptible at ______ months of age.
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bronchopneumonia, a superative abscessing pneumonia with large abscesses within the lungs
1-6 |
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Foals with Rhodococcus equi infections typically show symptoms of ________ and have a high mortality rate.
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dyspnea, fever, anorexia, rales
|
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Bacillus is a LARGE GPROD, of which there are many species, is common in the enviroment and is a (AEROBIC/ANAEROBIC/FACULTATIVE ??) bacteria that may be found as a common contaminant on culture plates.
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aerobic
|
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Bacillus anthracis, a LARGE GPROD is called __________, which is an obligate pathogen for animals and people.
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Anthrax
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Bacillus anthracis tends to affect________
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People, horses, cattle, pigs, dogs but rarely cats
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Bacillus anthracis is typically fatal to large animals and people if __________ but can be acquired through ________, which is less fatal in most cases
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inhaled
skin penetration and ingestion |
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Once Bacillus anthracis is in the body, the bacteria produce an EXOTOXIN that affects________
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CNS and CVS
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The EXOTOXIC effects of Bacillus anthracis are:
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~Damage to blood vessels causing cell and tissue death
~Fever and convulsions ~Edema-death due to suffocation from laryngeal edema ~Die quickly, usually w/in 24hrs ~May remain asymptomatic up until a few hours before death |
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The postmortem characteristics of Bacillus anthracis are:
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bleeding out of orifices
blood will not clot lack of rigor mortis |
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What are the three forms of Bacillus anthracis?
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1. Cutaneous
2. Pulmonary 3. GI |
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The cutaneous form of Bacillus anthracis is the least dangerous form that enters through a wound and in people is called " _________"
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Wool Sorters disease
|
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The pulmonary form of Bacillus anthracis is the MOST DEADLY because it involves:
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CVS collapse
coagulopathy hemorrhaging into the lungs shock and death 99% fatal w/in 24 hours |
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The GI form of Bacillus anthracis involves the ingestion of spores, which leads to:_____________ and is the most common form in dogs and cats, though rarely seen and not fatal.
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V/D
swelling of mouth and throat gastroenteritis |
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Treatment for Bacillus anthracis involves large dosages of _________ to surviving animals as well as quarantining of area for 3 weeks after last known case to prevent further outbreaks.
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antibiotics
|
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Bacillus anthracis is a disease that is reportable to the state. After seeing large GPRODS on a gram stain, tissues should be __________ and a necropsy ____________
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sent to the state lab
is NEVER performed due to potential for further spread of the disease. |
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Carcasses, bedding, contaminated dirt from a Bacillus anthracis outbreak is _____________
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burned or buried with a chemical disinfectant
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Clostridium is an anaerobic spore forming LARGE GPROD that releases ___________
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exotoxins
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Clostridium botulinum is also known as ___________ and tends to affect humans, mammals and waterfowl, esp. ducks.
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botulism
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Clostridium botulinum in birds is known as __________ and tends to affect waterfowl, shore birds, scavenger birds.
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Avian botulism ~ Limberneck
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Clostridium botulinum tends to be acquired by __________ that may be found in poorly canned foods, animal carcasses, rotting vegetation (pond bottoms) and even in maggots.
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ingestion of preformed EXOTOXIN in food source
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The pathology of Clostridium botulinum involves the neurotoxicity of the EXOTOXIN produced by this bacteria which acts at a neuromuscular junction to prevent the release of ____________
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acetylcholine ( a chemical that CAUSES muscle contraction)
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The endotoxin involved in Clostridium botulinum prevents the release of acetylcholine, which results in __________, which in animals typically starts in the _________
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muscle weakness and paralysis
hindquarters and progresses from there |
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Death from Clostridium botulinum is typically due to _________ and is ____% fatal
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respiratory arrest and suffocation due to paralysis of respiratory muscles and diaphragm.
95% |
|
Botulism antitoxin is available for people and horses, however, it is
|
EXPENSIVE
|
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Clostridium tetani, a LARGE GPROD, also known as tetanus & lockjaw is most prevalent in :
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(Most to least susceptible)
Humans and horses ruminants (+/- immunize) swine carnivores - rare poultry |
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The pathology of Clostridium tetani involves an neurotoxic endotoxin that blocks the function of __________, resulting in:
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inhibitory neurons
muscle contractions, spasms, rigidity |
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Clostridium tetani infection starts out with contractions and spasms occurring in response to _______
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startling stimuli such as loud noises or flashes of light
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If untreated, Clostridium tetani will cause death in ___________ from suffocation due to rigidity of respiratory muscles.
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1-2 weeks
|
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A person suffering from a tetanus infection will typically have:
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muscle spasms, lock jaw
|
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A horse suffering from tetanus will typically be seen in a "_________" stance with a rigid gate IF able to walk
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saw-horse
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Treatment for tetanus involves:
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tetanus antitoxin, antibiotics, muscle relaxers
|
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Prevention of tetanus involves:
|
an immunization with a tetanus toxoid and antitoxin if exposed.
|
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Name the 7 LARGE GPROD Clostridium bacteria
|
botulinum
tetani perfringes septicum chauvoei noveyi haemolyticum |
|
Clostridium perfringes , a LARGE GPROD, causes Clostridal enterotoxemia and tends to affect people, dogs and cats but is especially important in ___________
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large animals
|
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Clostridal enterotoxemia caused by Clostridium perfringes produces 12 different endotoxins and in addition to GI effects, can also cause ________
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wound infections and gas gangrene
|
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Clostridal enterotoxemia caused by Clostridium perfringes is acquired through ingestion of spores and some of the factors that can predispose to infection are:
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changes in diet, over eating, intestinal parasites, antibiotics, stress.
|
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Clostridal enterotoxemia caused by Clostridium perfringes affects intestinal environment so spores______
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become vegetative and then release exotoxins
|
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Clostridal enterotoxemia caused by Clostridium perfringes produces exotoxins that damage the MM of ________, which leads to hemmorrhagic enteritis and systemic_____
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intestines
toxemia |
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Clostridal enterotoxemia caused by Clostridium perfringes in large animals is often seen as the animal is 100% health the day before and then is suffering from:
|
hemorrhagic enteritis, systemic toxemia, ataxia, coma and death. Often found dead.
|
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Clostridal enterotoxemia caused by Clostridium perfringes is diagnosed by looking for __________
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large rods that resemble safety pins (3-5/hpf significant)
|
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Treatment for Clostridal enterotoxemia caused by Clostridium perfringes involves an ____________
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antitoxin to young LARGE animals if showing symptoms
antibiotics |
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Clostridium septicum is a histotoxic anerobic myocitis (muscle inflammation) that affects_____ and is acquired by endospores_______
|
large animals
entering wounds |
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Clostridium septicum pathology typically involves tissues necrosis, edema, gas gangrene and __________
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systemic toxemia and is highly fatal
**immunization is available |
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Clostridium chauvoei is a histotoxic bacteria that affects_________ and is also known as "__________" and is acquired through endospores being ingested or entering a wound
|
large animals
black leg disease |
|
The pathology of Clostridium chauveoi involves the EXOTOXINS causing swelling, edema, gas gangrene and the skin becoming _______, and eventually systemic toxemia leads to death.
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discolored
**immunization available |
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Clostridium novyi causes infectious necrotic hepatitis and affects _________, is also known as Black Disease, and is more common in areas with liver flukes.
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large animals
|
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Clostridium novyi is acquired through ingestion of the spores and pre-existing liver damage makes an animal _____________ and is highly fatal
|
more susceptible
**immunization available |
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Clostridium haemolyticum is also known as Bacilliary hemoglobinuria~red water disease, affects ______________.
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large animals
|
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Clostridium haemolyticum is acquired through ingestion of spores, and pre-exisiting liver damage makes an animal more susceptible. This bacteria releases and exotoxin which causes ______, which results in _________ and is highly fatal.
|
hemolysis of RBC's
dark red urine, anemia and high fever **immunization is available |