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16 Cards in this Set
- Front
- Back
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Mutagen
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anything that causes a mutation
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When damage is caused to DNA due to UV...
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UV is cut and replaced. thyamine dimers fix by moving the piece of DNA before transcription starts
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The only mutation that will change Amino Acids are...
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missense mutations
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Mutation that will stop Amino Acid sequence because it puts in a stop codon
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nonsense mutations
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Translation requires:
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Ribosomes, tRNA, and mRNA. it needs an AUG start codon and will be bound by tRNA and mRNA
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Recombinant DNA technology definition
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splicing and stitching to get DNA from other sources
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Methods of transferring
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1) Vertical transfer
2) Horizontal transfer=transferring DNA from one source to another and then having it produce offspring with that new gene |
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Transduction, two methods
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1) Generalized
2) Specialized--> example: shigella toxin found in endi 0157 H7 |
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If the professor uses the term ENZYME,
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he means protein.
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Restriction enzymes
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recognize certion DNA patterns and cut out
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Vectors have to be large enough to contain an entire bacterial genome, true or false?
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false.
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Vectors can be prokaryotic or eukaryotic. True or false?
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true
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requirements for PCR
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1) need DNA
2) need heat tolerance polymerase 3) need deoxyribonucleotides 4) need primers |
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PCR process:
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DENATURIZATION: heating up to denature and break bonds into helicase
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Vectors
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1) essential tools to clone a gene
2) plasmids and viruses can be used as vectors 3) Carry new DNA into desired cell 4) Shuttle vectors can exist in several different spaces |
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Gene cloning
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1) plasmid vector
2) enzymes that cut out the gene and ligate into a vector 3) transformation 4) use bacteria as factories to produce copius amounts of the gene |
