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51 Cards in this Set
- Front
- Back
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Klebsiella |
Gram Negative Oxidase Negative (Glucose Positive) Lactose Positive Non-Motile TSI: A/A H2S Negative Citrate Negative Grows only on HEA, BA and SCA (blue) |
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Escherichia |
Gram Negative Oxidase Negative (Glucose Positive) Lactose Positive Motile TSI: A/A H2S Positive IMViC: + + - - Grows on SCA, BA, MA, BE, HEA |
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Yersinia |
Gram Negative Oxidase Negative (Glucose Positive) Lactose Negative Motile TSI: A/A H2S Negative IMViC: + + - - Grows on BA, MA, HEA |
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Proteus |
Gram Negative Oxidase Negative (Glucose Positive) Lactose Negative Motile TSI: A/A H2S Positive Grows on BA (alpha), MA |
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Salmonella |
Gram Negative Oxidase Negative (Glucose Positive) Lactose Negative Motile TSI: Alk/A H2S Positive Grows on SCA, BA, MA, BE, HEA |
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Serratia |
Gram negative Oxidase Negative (Glucose Positive) Lactose Negative Motile TSI: Alk/A H2S Negative Grows on BA (red), MA, HEA |
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Pseudomonas |
Gram negative Oxidase Positive (Glucose Negative) Lactose Negative Motile TSI: Alk/Alk H2S Negative |
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Staphylococcus Aureus |
Gram Positive Cocci Catalase Positive Coagulase Positive Pathogenic Grows on BPA (black halos), BA (beta), MSA |
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Staphylococcus Epidermis |
Gram Positive Cocci Catalase Positive Coagulase Negative Grows on BA (gamma), MSA |
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Enterococcus |
Gram Positive Cocci Catalase Negative Grows on BA (gamma), BE (black precipitate) |
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Bacillus Cereus |
Gram Positive Bacilli Motile Catalase Positive Grows on BA (big fat beta) |
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Listeria |
Gram Positive Coccobacilli Catalase Positive Motile (at 4'C) Beta Hemolysis Grows on BA (narrow beta), BE (black precipitate) |
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What are you going to write with? |
PEN |
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Where are you going to write in? |
The boxes provided |
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Are you going to reread the questions? |
Yes |
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Motility Tests are done for which type of Bacteria? |
Bacilli |
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Oxidase tests are done for which Gram? |
Gram Negatives |
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Catalase tests are done for which Gram? |
Gram Positives |
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Mannitol Agar |
S: NaCl, selects against non-Halophiles D: Alters Phenol Red, Mannitol Fermentation Staph Aureus: Yellow, MF, Coag. +, Pathogenic Staph Ep.: Pink,NMF, Coag. -, Non-Pathogenic Enterococcus: Inhibited by Salt |
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Mac #3 |
S: Bile Salts, Crystal Violet D: Neutral Red (Indicator), Lactose E Coli: LF (Pink) Salmonella: NLF (Colourless) Enterococcus Faecalis: NLF, Inhibit by CV |
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Bile Esculin |
S: Against MOST Gm +, Bile salts D: Species Breakdown Esculin into Esculite which hydrolyzes Ferric Citrate making it black precipitate Enterococcus Faecalis: Positive, Black precipitate Listeria Monocytogenes: Positive, Black Prepcip. E coli: Negative, no black precip. (might grow) |
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Baird-Parker |
S: Glycine, Sodium Pyruvate D: Egg yolk, tellurite breaks down meats and fats Staph. Aureus: Positive, 2 halos, coag. +, patho Staph Epid.: Positive, 1 halo, coag. -,non-patho
2 rings: Proteolytic and lipolytic 1 ring: lipolytic |
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Hektoen Enteric |
E: Yes S: Bile Salts D: NaCl, Sodium thiosulphate for h2s production, dye: Acid Fuschin, Bromothymol Blue Salm. Enterica: +ve, green fish eye, from H2S E coli (spp dependent): +ve, yellow/orange, rapid lactose and sucrose fermentation |
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Blood Agar |
Enriched: Yes Selective: No Differential: Yes, hemolysis and colony sizes |
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Tsi testing: What are for things to check? |
Slant: Alkaline (Red) or Acid (Yellow) Glucose test Butt: Alkaline (Red) or Acid (Yellow) Lac &/or Suc test Gas: Are there cracks in agar? H2S: Is there black precipitate? |
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Motility: Do you do motility tests on cocci? |
No you don't they're non-motile |
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Motility: Do you do motillity tests on bacilli |
Yes you do, they're motile |
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Citrate Test: Positive and Negative |
Blue: Positive Green: Negative |
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TSI is used for what Grams? |
TSI is used for Gram negatives only |
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What does Green and Yellow/Orange mean on Hektoen Enteric? |
Green: H2S release Yellow/Orange: Rapid Lactose and Sucrose fermentation |
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Salmonella Chromogenic, purple to pink and blue? |
Blue: Coliforms Purple to Pink: Salmonella Species |
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Mac #3, Pink and Colourless? |
Pink: Lactose fermentation Colourless: No Lactose Fermentation (Changes Agar colour too) |
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Mannitol, Yellow and Pink? |
Yellow: Positive for coagulation, MF & Patho. Pink: Negative for coagulation, MF & Patho. |
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Bile Esculin, What's black precipitate? |
Black precipitate is the species breaking down Esculine into Esculite that hydrolyzes Ferric Citrate to cause black precipitate |
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Should you use BA for Catalase tests? |
No, the hema in blood will also catalase |
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What does Bile Esculin select against? Why? |
MOST gram positives. Due to the Crystal Violet and Bile Salts |
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What does MacConkey Agar select against? Why? |
MOST gram positives. Due to the Crystal Violet and Bile Salts |
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What grows on Baird-Parker? |
Staph Species |
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You do gram testing and it's a negative, oxidase positive and motile. What is it? |
Pseudomonas aeruginosa |
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You do gram testing and it's a negative, Lactose negative. What's your next suggested test? |
TSI: - Proteus: A/A, H2S Positive - Yersinia: A/A, H2S Negative - Salmonella: Alk/A, H2S Positive - Serratia: Alk/A, H2S Negative
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You do gram testing and it's a negative, Lactose positive, and it's mucoid on BA and encapsulated on the microscope. What is it? |
Klebsiella |
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You do gram testing and it's a negative, Lactose positive. What would be the following test? |
Citrate: - E coli: negative - Enterobacter: Positive |
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You do gram testing and it's a positive, it's also a bacilli. What is it? |
Bacillus cereus: rod shape Listeria monocytogenes: coccobacilli |
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You do gram testing and it's a positive, it's also a cocci. What is the next test? |
Catalase test: - Staph spp.: Positive - Enterococcus: Negative |
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Centrimide agar |
E: No S: Yes, for Pseudomonas aeruginosa. Phenazine pigment causes florescent colors D: No |
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Brilliant Green Agar |
E: Yes, sucrose S: Yes, Brilliant Green inhibits gram negatives (aside from shigella) (and supresses coliforms) D: Phenol Red is used for different species of salmonella |
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Listeria Brilliance |
E: Yes S: Yes, Polymyxin B, Nalidixic acid used for selective reagents D: Yes, Lecithin solution to differentiate between Listeria species |
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CIN (Yersinia) agar |
E: No S: Yes, cefsulodin, irgasan, and novobiocin D: Yes, Crystal Violet |
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Bismuth Sulphite agar |
E: Yes S: Yes, for salmonella species, brilliant green suppresses coliforms D: Bismuth Sulphite indicator and Brilliant Green for isolation of Salmonella spp. |
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Sab Dex agar |
E: No S: Yes, low pH, D: No |
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TSA agar |
Enrichment for various amount of bacteria |
