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18 Cards in this Set
- Front
- Back
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what is a smear
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a thin film of a solution of microbes on a slide
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why is a smear usually fixed
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to attach the microbes to the slide and to kill the microbes
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how do researchers make discoveries about cell behavior
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by observing live specimens
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what do stains consist of
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a positive and negative ion
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the chromopore is a cation in a
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basic dye
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the chromophore is an anion in a
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acidic dye
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what is negative staining
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staining the background instead of the cell
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what type of staining uses a single basic dye
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simple stain
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what is a mordant used for
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to hold the stain or coat the specimen to enlarge it
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the gram stain classifies bacteria into
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gram-positive or gram-negative
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gram-positive bacteria tend to be
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killed by penicillin and detergents
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gram-negative bacteria are more
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resistant to antibiotics
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what are the basic steps to gram staining
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1. apply crystal violet to the slide
2. apply iodine (mordant) 3. alcohol wash (decolorization) 4. apply safranin (counterstain) |
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what are cells that retain a basic stain in the presence of acid-alcohol called
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acid-fast
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non-acid fast cells lose the basic stain when rinsed with bacteria and are usually ______ to see them
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counterstained
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what staining is useful for capsules
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negative staining
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what is required to drive a stain into endospores
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heat
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what requires a mordant to make the flagella wide enough to see
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flagella staining
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