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18 Cards in this Set

  • Front
  • Back
what is a smear
a thin film of a solution of microbes on a slide
why is a smear usually fixed
to attach the microbes to the slide and to kill the microbes
how do researchers make discoveries about cell behavior
by observing live specimens
what do stains consist of
a positive and negative ion
the chromopore is a cation in a
basic dye
the chromophore is an anion in a
acidic dye
what is negative staining
staining the background instead of the cell
what type of staining uses a single basic dye
simple stain
what is a mordant used for
to hold the stain or coat the specimen to enlarge it
the gram stain classifies bacteria into
gram-positive or gram-negative
gram-positive bacteria tend to be
killed by penicillin and detergents
gram-negative bacteria are more
resistant to antibiotics
what are the basic steps to gram staining
1. apply crystal violet to the slide
2. apply iodine (mordant)
3. alcohol wash (decolorization)
4. apply safranin (counterstain)
what are cells that retain a basic stain in the presence of acid-alcohol called
non-acid fast cells lose the basic stain when rinsed with bacteria and are usually ______ to see them
what staining is useful for capsules
negative staining
what is required to drive a stain into endospores
what requires a mordant to make the flagella wide enough to see
flagella staining