Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
24 Cards in this Set
- Front
- Back
|
First Person to See Microorganisms
|
Anton van Leeuwenhock
|
|
|
the technology of making very small things visible to the human eye.
|
Microscopy
|
|
|
ability to see two items as separate and discrete units
|
Resolution
|
|
|
a mathematical expression relating to the extent that light is concentrated by the condenser lens and collected by the objective.
|
Numerical Aperture (NA)
|
|
|
the passage of light through and object
|
Transmission
|
|
|
energy from absorbed light can raise the of an object.
|
Temperature
|
|
|
UV rays are changed into longer wavelengths and reemitted
|
Luminescence
|
|
|
object continues to emit light when light rays no longer strike it
|
phosphorescent
|
|
|
the bending of light as it passes from one medium to another of different density
|
Refraction
|
|
|
measure of the speed at which light passes through the material.
|
Index of Refraction
|
|
|
As light passes through a small opening, the light waves are bent around the opening.
|
Diffraction
|
|
|
microscope with more than one lens
|
Compound Light Microscope
|
|
|
lens that magnifies the image before it passes through the body tube
|
Objective Lens
|
|
|
Lens that further magnifies the image
|
Ocular Lens
|
|
|
makes use of differences in refractive index to visualize unstained cells and structures.
|
Nomarski microscopy
|
|
|
UV light is used to excite molecules so that they release light of a longer wavelength than that originally striking them.
|
Fluorescence microscopy
|
|
|
staining that determines whether an antigen is present.
|
Fluorescent Antibody staining
|
|
|
heavy metal is sprayed at an angle onto a specimen.
|
Shadow casting
|
|
|
In this technique, the cell is frozen and then fractured with a knife.
|
Freeze-Fracturing
|
|
|
a drop of medium containing the organisms is placed on a microscope slide; used to view living organisms
|
Wet mounts
|
|
|
loopful of medium are spread onto the surface of a glass slide; used to view killed organisms
|
Smears
|
|
|
Reveals basic cell shapes and cell arrangements
|
Simple Stain
|
|
|
distinguishes between two kinds of organisms
|
Differential Stain
|
|
|
bacterial cells take up crystal violet; differential stain; iodine is added
|
Gram stain
|
