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15 Cards in this Set
- Front
- Back
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blood agar
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alpha hemolysis: green
beta hemolysis: yellow no hemolysis: nothing |
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eosin methylene blue agar
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lactose fermenting: nucleated colonies
e coli: green metallic sheen |
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macconkey agar
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lactose fermenting: pink
non lactose fermenting: clear |
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salmonella shigella agar
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lactose fermenting: red
non lactose fermenting: black |
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carbohydrate fermentation
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acid: yellow
no acid: red gas/no gas |
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catalase
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add drop of hydrogen peroxide
bubbles (+) or no bubbles (-) |
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citrate utilization
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grow bacteria in citrate medium wtih bromthymol blue
blue (+) or nothing (-) |
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coagulase
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mix bacteria with plasma
clot (+) or no clot (-) |
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decarboxylase
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decarboxylation releases carbon dioxide and amine
medium contains amino acids and bromcresol purple indicator purple (+) |
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gelatin liquefaction
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proteases hydrolyze and destroy gel
gel liquefied (+) or unchanged (-) |
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indole
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convert tryptophan to indole
red (+) |
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methyl red
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acid fermenters cause pH to drop below 4.3. add methyl red indicator
red (+) |
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starch hydrolysis
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iodine-iodide detects starch. grow on plate containing starch. if organism hydrolyzes starch, iodine will not detect it; will be clear. if iodine detects starch, will turn blue.
blue (+) or clear (-) |
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urease
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urease splits urea. innoculate medium with urea and phenol red indicator.
red (+) |
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vogues-proskauer
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sugar fermentation produces acetoin. alpha napthol indicator.
red (+) |
