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34 Cards in this Set

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Read: Reverse Electron Flow by Chemoautotrophs


- Many energy sources used by chemo-lithotrophs have E0 more positive than NAD+(P)/NAD(P) so how do they make NADPH? Use reverse electron flow to make NADPH for biosynthesis.

:-)

Read over diagram slide 3 (lec 16)

:-)

Hydrothermal vent communities are based on what?

Chemolithotrophy (chemolithoautotrophs)

Populations of chemolithotrophic bacteria can be found in deep sea hydrothermal vents, and often live in symbiotic relationships with other invertebrates. Tube worms (Riftia pachyptila) and clams (Calpytogena magnifica) who live in association with chemolithotrophs at the bottom of the sea have a unique type of _______ that provides the bacteria with a source of _______ and ________ necessary for chemoautotrophic metabolism. The bacteria, in turn, provide the worms and clams with nutrition since all digestive system features are ________.

hemoglobin


oxygen


hydrogen sulfide


absent

________: uses light as energy source

phototrophy

________: light mediated ATP synthesis

photophosphorylation

Use ATP for assimilation of CO2 biosynthesis

Photoautotrophs

Use ATP for assimilation of organic carbon for biosynthesis

photoheterotrophs

Read: MOST phototrophs also fix carbon, but some can use or even require organics – all about niche utilization

:)

Read over slide 10 (lec 16)

:)

Phototrophy in archaea occurs in the _______. It is not _______ based. They use ________ - a membrane bound solar powered proton pump!

halophiles


chlorophyll


bacteriorhodopsin

Read table in slide 11 (lec 16)

:)

Photosynthesis - Eukaryotes vs Bacteria


Comparison of photosystems between Eukaryotes & bacteria:


- _________ evolve O2: much like ________ in eukarya. And like (______), use ___ photosystems.


- Other bacteria use electron donors other than ______ which makes them anoxygenic. They run __ photosystem which is either cyano ___ OR ___ -like.

Cyanobacteria


chloroplasts


(chloroplasts)


2


H2O


1


PSI


PSII

What are the 5 major bacterial phyla of anoxygenic phototrophs?

Proteobacteria


Chlorobi


Chloroflexi


Firmicutes


Acidobacteria

The proteobacteria contain which 2 type of bacteria?

Purple sulfur bacteria


purple non-sulfur bacteria

In purple sulfur bacteria, ______ (mostly) or ___ is e- donor.

H2S


S0

In purple non-sulfur bacteria _____ (mostly) is e- donor

H2

Chlorobi contain _______ which have ____ mostly or ____ as e- donor

green sulfur bacteria


H2S


H2


Chloroflexi:


________ bacteria


_______ as e- donor

green non-sulfur


H2

Describe the firmicutes

Gram positive (low G+C)

The Acidobacteria is a new group that is abundant in _______.

soils

Chlorophylls are similar to heme porphyrins of cytochromes, but have _______ instead of ______ at the center.

magnesium


iron

At least 7 different bacteriochlorophylls known to exist - have different _________ properties.


- allow phototrophs to coexist in same habitat harvesting ____________ of light.


- _________ alcohol anchors the chlorophyll into the membrane

absorption


different wavelengths


hydrophobic

Accessory Pigments


-Aid in _________ (mainly; quench toxic oxygen species that arise from photooxidation) and ______ ________ (e.g. carotenoids & phycobilins) - and give other pretty colors!

photoprotection


light harvesting

Accessory Pigments


-In cyanobacteria ________ and ____ are responsible for their characteristic blue-green color (remember they were once called blue-green algae!)

phycocyanins


chl a

Photosynthesis


Energy from light trapped and converted to chemical energy


- two part process


1. light reactions: light energy is ______ and converted to ________ energy.


2. dark (light independent) reactions: energy produced in the light reactions is used to reduce _____ and synthesize cell constituents.

trapped


chemical


CO2


Light harvesting mechanics


- In _______ & anoxygenic ________ phototrophs, pigments arranged in large complexes complexes with proteins.


- _____ percentage of complexes actually form the reaction center, the rest funnel the energy to the reaction center


cyanos


purple


small

Look over pictures on slide 19 (lec 16)

:-)

Light harvesting mechanics


- Chlorophyll pigments may be on membrane _____ or located _____ special membrane extensions


- in ___________ & _________, they are called ________ (extensive invaginations of cytoplasmic membranes).


- In the purple phototrophs, _____ or ________ of membranes - extensions of membrane.


surface


within


eukaryotes


cyanobacteria


thylakoids


vesicles or stacks

Light harvesting mechanisms


________ function as massive antenna complexes.


What type of bacteria are they found in?


-Bchhl, c, d, & e are _____ attached to proteins.


They run the length of the structure, passed to _____ in reaction center.


- Allow to live at very _________ that wouldn't support any other phototroph.


- ______ in aquatic environments


- deep within __________ ______.

Chlorosomes


Found in green sulfur bacteria & green nonsulfur bacteria


not


Bchl a


low light intensities


at depth


microbial mats

Look at picture on slide 21 (lec 16)

:-)

Light Reactions in Oxygenic Photosynthesis


- Photosynthetic ______ & ________: ____ photosystems, that operate in tandem. Generate ______ & ______ ---> ATP


- What is then used to fix CO2?


- _______ is generated and released into the environment as a ______ pdt.

eukaryotes & cyanobacteria


2


NADPH & PMF


NADPH & ATP


oxygen


waste

Oxygenic Photosynthesis


Energy conserved by:


1) ________ ______flow


-ATP & NADPH made (______ photophosphorylation)


2) _____ ______ flow


-ATP made via ____ photophosphorylation



noncyclic electron


noncyclic


cyclic electron


cyclic

Look over diagram slide 23 (lec 16)

:)