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24 Cards in this Set
- Front
- Back
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Clinical presentation of Streptococcal Pharyngitis
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Inflamed and edematous pharyngeal mucosa.
throat pain, difficulty swallowing, fever, headache, occasionally scarlatiniform rash. |
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What is the most common cause of pharyngitis?
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Group A B-hemolytic stretococci
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alpha hemolytic detection setup
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stap the 5% sheep blood agar plate. This creates an anaerobic environment,.
B hemolysis completely destroy and decolorize hemoglobin. A hemolysins partially destroy hemoglobin, leaving a greenish area around the colony. |
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Taxo A and P disks
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GAS are inhibited by bacitracin, which is on Taxo A disks.
Pneumococci are inhibited by Optochin, which is on Taxo P disks. |
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What is GAS?
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Group A Strep
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What are the standards for judging Positive Taxo A and Positive Taxo P?
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Positive A is any area of inhibition.
Positive P is an area of inhibition beyond 14mm. |
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What is another name for Stretococcus pneumoniae?
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Pneumococcus
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Alpha and Beta and pneumo and group A strep
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Group A Strep is Beta Hemolysins
Pneumo is Alpha hemolysins |
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Which colonies totally destroy the area around them?
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Beta.
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Symptoms of Group A Strep
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Suppurative (pus forming)
GAS causes pharyngitis, scarlet fever, bacteremia, skin infections as well as many other kinds of infections. GAS also is the cause of necrotizing fasciitis, or "flesh eating bacteria" |
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Symptoms of Pneumococci
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pneumonia, bacteremia, meningitis, otitis media.
ppl with pneumonia have rust colored sputum tinged with blood. |
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what exactly happens in hemolysis and what kinds are there?
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3 kinds. alpha, beta, and gamma.
in beta, hemoglobin is completely destroyed. in alpha, hemoglobin is partially destroyed. in gamma, no hemoglobin is destroyed. |
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what does TSI stand for?
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Triple Sugar Iron Agar
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What does mineral oil do?
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creates anaerobiosis, or tests for anaerobic reactions.
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what color change to carbohydrate tests undergo in API?
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from blue/green to yellowif positive.The thing being tested it fermentation / oxidation.
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which API test slots have mineral oil?
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The anaerobic ones. The ones with underlines on them.
ADH LDC ODC H2S URE |
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Which API test slots fill all the way?
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The ones with the cup image.
CIT VP GEL |
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Chemicals coming from GAS colonies (or strep pyogenes)
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Streptolysin S and streptolysin O.
Ones with O only tend to need anaerobic conditions to fully grow. |
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what color change does the decarboxylase test have?
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from yellow to red/orange
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What are the 3 reagents we used for our API tests and what tests were they?
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INDOLE test = Kovac's
VP test = KOH and alpha-naphthol TDA = Ferric Chloride |
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Strep throat is most commonly caused by what kind of bacteria?
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Strep A pyogenes
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Clinical presentation of pyogenes / pharyngitis
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inflamed mucosa. throat pain. difficulty swallowing.
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On the ONPG test, what is the color of positive?
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yellow
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On the ONPG test, what is the enzymatic activity tested?
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beta-galactosidase
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