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27 Cards in this Set

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all broken glass/glass to be disposed of goes in the _____.
Broken glass box
petri dishes, swabs, etc, go in the ____.
autoclavable box
you should ___ inoculation tubes
save/recycle
total magnification=
ocular mag x objective lens mag
4x magnification objective
scanning objective
10x magnification objective
low power objective
40x magnification objective
high, dry objective
100x magnification objective
oil objective
Resolution=_____
d=(λ)Wavelength/2xNumerical aperture
with any microscope use you want ___ resolution
small
what are the ways we can get a small resolution?
1. make λ small
2. make numerical aperture big
3. use oil
what does a blue filter give you?
smaller wavelength (λ) of light
why does oil give you a small resolution
oil has the same refraction index as glass, the oil keeps the light from bending
the objectives are all in focus
parfocal
what is the difference between eucaryotes and procaryotes?
eucaryotes have organelles
what two groups are the Eucaryotes broken down into? Whats the domain?
-protozoans and algae
-Domain=protists
-type of protist
-typically motile
-heterotroph
protozoans
-type of protist
-nonmobile
-phototrophs
algae
what two domains are procaryotes divided into?
-eubacteria (bacteria)
-archea
the size of a typical bacterium
1-10 microns
size of a typical protist
10-50 microns
size of a RBC
7-10 mircrons
size of a virus
nanometers
resolution of a light microscope
0.2 microns
resolution of the human eye
200 microns
does asceptic=sterile?
NO!!!
to work with a desired microbe but avoid contamination by working with pure (only 1 type) cultures
Asceptic technique