Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
27 Cards in this Set
- Front
- Back
all broken glass/glass to be disposed of goes in the _____.
|
Broken glass box
|
|
petri dishes, swabs, etc, go in the ____.
|
autoclavable box
|
|
you should ___ inoculation tubes
|
save/recycle
|
|
total magnification=
|
ocular mag x objective lens mag
|
|
4x magnification objective
|
scanning objective
|
|
10x magnification objective
|
low power objective
|
|
40x magnification objective
|
high, dry objective
|
|
100x magnification objective
|
oil objective
|
|
Resolution=_____
|
d=(λ)Wavelength/2xNumerical aperture
|
|
with any microscope use you want ___ resolution
|
small
|
|
what are the ways we can get a small resolution?
|
1. make λ small
2. make numerical aperture big 3. use oil |
|
what does a blue filter give you?
|
smaller wavelength (λ) of light
|
|
why does oil give you a small resolution
|
oil has the same refraction index as glass, the oil keeps the light from bending
|
|
the objectives are all in focus
|
parfocal
|
|
what is the difference between eucaryotes and procaryotes?
|
eucaryotes have organelles
|
|
what two groups are the Eucaryotes broken down into? Whats the domain?
|
-protozoans and algae
-Domain=protists |
|
-type of protist
-typically motile -heterotroph |
protozoans
|
|
-type of protist
-nonmobile -phototrophs |
algae
|
|
what two domains are procaryotes divided into?
|
-eubacteria (bacteria)
-archea |
|
the size of a typical bacterium
|
1-10 microns
|
|
size of a typical protist
|
10-50 microns
|
|
size of a RBC
|
7-10 mircrons
|
|
size of a virus
|
nanometers
|
|
resolution of a light microscope
|
0.2 microns
|
|
resolution of the human eye
|
200 microns
|
|
does asceptic=sterile?
|
NO!!!
|
|
to work with a desired microbe but avoid contamination by working with pure (only 1 type) cultures
|
Asceptic technique
|