Microbio Exam 1

Front 1

Who first published extensive observations of micro-organisms?

Back 1

Anton von Leeuwenhoek

Front 2

Who first suggested using the thickening agent most commonly used for colony purification today?

Back 2

Fannie Hesse

Front 3

The most important innovation (new idea) in Pasteur's 'swan neck flask' experiments was:

Back 3

fresh air could directly contact the medium.

Front 4

Agostino Bassi first showed that a disease, in silkworms, was caused by microorganism. What kind of microorganism caused the disease?

Back 4

Fungus

Front 5

What was the first virus shown to cause disease?

Back 5

Tobacco mosaic virus

Front 6

Living, unstained cells and organisms can be observed best using

Back 6

phase contrast microscopy

Front 7

Resolution is the ability of a lens to distinguish between small objects close together. What approximate resolution can be obtained with a lower power (10X, N.A. 0.25) objective lens?

Back 7

0.9 microns

Front 8

Which of the following statements about Transmission Electron Microscopy is NOT true.
a.)The specimen must be stained with osmium or other heavy metal.
b.) The specimens are placed in a high vacuum for viewing.
c.) The specimens must be sliced very thin, 20-100 nm in thickness.
d.) The beam is focused by electromagnetic lenses.
e.) All of the above are true.

Back 8

all are true

Front 9

The phase contrast microscope is best for visualizing live, motile microorganisms.
True
False

Back 9

true

Front 10

Fluorescence microscopy is based on the concept that some molecules absorb radiation and reemit it at a shorter wavelength.
True
False

Back 10

False