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10 Cards in this Set
- Front
- Back
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Sterilization:
What does it kill? |
kills all types of microorganisms including spores but NOT prions
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Disinfection:
What does it kill? |
kills all types of microorganisms excluding spores and prions (weaker than sterilization)
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Sterilization:
6 Techniques? |
-dry heat
-autoclave -ionizing rays (x and gamma) -filtration -ethylene oxide (gas sterilization) -glutaraldehyde |
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Autoclave Sterilization:
-Temperature? -Pressure? -Time? -Mode for Killing? |
-121 deg C at 15 psi for 15-20 min OR
-134 deg C at 30 psi for 3 min (flash cycle) -kills by causing thermal coagulation of proteins |
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Ethylene Oxide (Gas) Sterilization:
-Temperature? -Special Uses? -Mode for Killing? |
-below 60 deg C
-used for heat and water sensitive objects -alkylating agent and kills by damaging proteins and nucleic acids |
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Glutaraldehyde Sterilization:
-Also known as? -Method? -Mode for Killing? |
-aka "cold sterilizer"
-submerged for 10 hours in 2% glutaraldehyde -alkalizing agent |
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Spore Tests:
What spore is used? |
non-pathogenic bacillus spores are used
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Disinfection:
3 Techniques? |
-formaldehyde
-alcohols -oxidizing agents |
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Substances used for destroying microorganisms on...
-non-living objects? -living tissue? |
-non-living: disinfectants
-living tissue: antiseptics (eg alcohol) |
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Pasteurization:
Goal? |
goal: logarithmic reduction in number or organisms (not kill all)
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