**micro Test 2 - Lecture 1 - Oral Biofilm

Front 1

Oral streptococci
gram?
anaer/aer?
where to they inhabit?

Back 1

+
fac anaer
oral/upper resp

Front 2

what strep bact causes caries?

Back 2

mutans

Front 3

what strep bact causes abscess?

Back 3

angionosus

Front 4

what strep bact causes gingivitis?

Back 4

s sanguis

Front 5

what strep bact causes pneumonia?

Back 5

pneumoniae

Front 6

what strep bact causes bacteremia/endocarditis?

Back 6

viridans strep

Front 7

viridans strep causes

Back 7

bacteremia/endocarditis

Front 8

strep angionosus causes

Back 8

abscess

Front 9

s sanguis causes

Back 9

gingivitis

Front 10

oral plaque has over how many species

Back 10

700

Front 11

sequencing of what rna has allowed id of plaque bact

Back 11

16s ribosomal sequencing

Front 12

how is the bacteria that they think is in plaque viewed?

Back 12

via FISH

Front 13

FISH characteristics
benefits are that it can detect ____populations, it is ____specific, and that it is culture ______.

Back 13

low
species
independent

Front 14

FISH characteristics
drawbacks are that you can only do it on____volumes of bacteria, it is technically ______and that you 1st need to do_____.

Back 14

low
challenging
DGGE or LIBRARY

Front 15

DGGE gives you four thngs
__a__complexity
community __b___by sequencing
distribution of microbial populations in ___c___
monitory ____d____

Back 15

a - community
b - members
c - different environments
d - community changes

Front 16

put the steps of sequncing in order
a - Dna extraction
b --design probe for rRNA region of interest
c - -take sample
d - - PCR 16s giving amplicons
e - DGGE or Clone library and compare to databse

Back 16

c - a - d - e - b

Front 17

Microarrays are unique in that they make use of _______.

Back 17

cy5 labeled 16s primers

Front 18

cy5 labeled 16s primers are unique to

Back 18

microarrays

Front 19

454 pyrosequencing allows sequencing of _____

Back 19

all dna

Front 20

the new seq technique allowing seq of ALL dna that uses A/B linkers and a bead sequence is

Back 20

454 pyrosequencing

Front 21

primary colonizer of biofilm
secondary colonizer
tertiary colonizer

Back 21

strep + pellicle

f nucleatum

P gingivalis and other g-

Front 22

Why does f nuc and p gingivalis need strep to bind first?

Back 22

it uses up all the o2 and they are fac anaerobes

Front 23

what does strep do to kill of s sobrinus?

Back 23

releases MUTASINS

Front 24

strep mutasins kill of nice bacteria, such as

Back 24

s sobrinus

Front 25

since s. cristatus can't itself invade epith cells, it does it via

Back 25

f nucleatum

Front 26

Ecological zones are high biomass sites which are defined by areas that ____a____ or low biomass zones tha __B___ or saliva, which is a ___C___

Back 26

don't shed (tooth)
do shed
transitional zone

Front 27

spirochetes, bacterioides, propionibacteria are characteristic of what type of disease

Back 27

periodontitis

Front 28

enamel demineralizes at pH of ____and dentin at pH of ____

Back 28

5.5 enamel
6.5 dentin

Front 29

how does the host response differ to primarily G+ plaque?
to G- plaque?

Back 29

g+ - works to protect tissue
g- - destruct bone

Front 30

perio disease has over ___bacteria per pocket

Back 30

10^8

Front 31

ludwig's angina
80% of patients ahve had ____-

Back 31

recent dental work

Front 32

ludwig's angina
infection first seen in ____spaces, then moves to _____.

Back 32

submand/subment
lat/retro pharyngeal

Front 33

major complication of ludwigs

Back 33

asphyxia

Front 34

ludwig angina
anaerobes are __%

Back 34

75

Front 35

peptostreptococci
bacteroides
f nucleatum
3 bact of _____

Back 35

ludwigs

Front 36

alpha hemolytic, MRSA are bacteria of ____

Back 36

ludwigs