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26 Cards in this Set
- Front
- Back
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what does EMB stand for?
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Eosin Methylene Blue
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What is the purpose of an EMB agar? What are the ingredients?
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It has lactose in it. It grows G (-) organisms...
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What color is an EMB?
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Purple. And colonies are Dark. i.e. Metallic Green
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Which organisms lookk like fish eyes, and which medium do they grow on?
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Enterobacter and Klebbselia. Enterobacter is a dark/black colony, and Klebbselia is a gooey center. They both grow on EMB.
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What does MSA stand for?
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Manitol Salt Agar
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What does MSa grow?
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Halo-tolerant organisms.
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What is the percentage of salt in MSA?
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7.5%
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on an MSA medium, what color are the two staphs?
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STaph Ep-(G positive) ACID YELLO....beecause it breaks down the manitol..
Staph AU-(G negative) Stays RED because it doesn't break down manitol. |
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Which organism causes bed ulcers?
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Pseudomonas Aeruginosa
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Which type of organism causes "gull wing" formations...
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Genus: Microaerophiles...
Species:Gastro Enteritus...camplobacter jejunii |
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What is another name for a fastidious organism?
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capneic
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What grows on an acidic medium?
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yeasts and molds...pH 0-1: Thiobacillus Thiooxidans..., and Alcaligenes viscolactis
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What percentage of salt can Extreme halophiles live in?
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15-20 %
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What percentage of salt can Low level halophils live in?
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3.5%-4.0%
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Who discovered Anaerobiosis
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PASTEUR
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What is the log phase?
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the first 4 hours...it will log adjust to the media...no division, organisms are just getting larger
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What is the second phase?
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Logarithmic phase: 4-24 hours...They multiply. Generation time calculated.
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What is the third phase?
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24-48 hours...plateau phase...Stationary.
It runs out of a nutritional source, or maybe it's building up toxic products...too much acid...pH is lowered, and metabolism stops. Cells dividing at the same rate as cells dying. |
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How many ATp's are produced in glycolysis?
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2....2 are expended, and four are produced...4-2=2
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What does Glycolysis do?
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Takes glucose to pyruvate, and provides energy...(2ATP)
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How many ATP does Ecoli get from the Kreb's cycle
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ecoli gets: 38 ATP
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How many ATP do we get from the Kreb's cycle?
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36...
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After spinning the Kreb's cycle 2x, how many NADH are there?
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8 NADH
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Why do we need GTP?
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For DNA and RNA, and Protein Synthesis.
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How many ATP's are there per NADH?
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3 ATP's per NADH.
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What is the end result of THe ETS?
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Oxygen, and WATER
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