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66 Cards in this Set

  • Front
  • Back
why do we study microbiology?
disease, food products, food spoilage, nutrient cycles
what food products can be made using microorganisms?
cheeses, bread, wines, beer, distilled alcohol, yogurt, sour cream, buttermilk, acidophilus milk, sauerkraut, soy sauce, green olives, some pickles, some hard sausages, vinegar
explain the carbon cycle
carbon dioxide to sugass to carbohydrates to carbon and back.
some animals that use microorganisms in nutrient cycling
cows, horses, termites
explain the nitrogen cycle
N2 to ammonia, nitrate, nitrite to amino acids, proteins, nucleic acids to nitro compou D's and back.
only---------------can biotically fix atmospheric nitrogen
microorganisms
food poisoning
food intoxication
food born illness
ingested bacteria that now lives inside you
azotobacteria spp.
free living- not symbiotic relationship
rhizobium spp.
symbiotic with roots of legumes.
genus
the first word capitalized
species
second word lower case
how names should always be written
in italics or underlined
saprophytes
organisms which obtain their nutrients from the decomposition of dead plants and animals
microorganisms are used in-----------treatment
waste and sewage
industrial level products made by microorganisms
vitamins, solvents, alcohol, antibiotics, methane
theory of spontaneous generation
living things can arise from non living matter
examples of false proof of spontaneous generation
maggots from decaying meat, mice from soiled clothing and grains, snakes from horse hair in stagnant water
Francesco redi
partially disproved spontaneous generation. good example of scientific method
Anton van leeuwenhoek
first to observe microorganisms, made Hus own lenses, and used drops of pond water teeth scrapings, feces, infusions(peppercorn, hay, dried beans)
Leeuwenhoek's contrtbutioins
demonstrates existence of microscopic life, discovered microorganisms are different shapes and sizes, and Re virtually everywhere
Robert hooke
firs to observe cells
Matthias schleiden and Theodor schwann
credited with cell theory of biology-all living things are composted of cells
Rudolf virchow
theory of biogenesis-all living cells arise only from preexisting living cells
Louis paster
disproved theory of spontaneous generation. hypothesized that there were microbes in the air. swan neck flask.
germ theory of disease
germs can cause disease.
people who helped develop germ theory of disease
Agostini bassi- discovered fungus that caused disease in silkworms
Ignaz semmelweis- father of hand washing. childbed fever.
john snow
"discovered" what cause cholera outbreak. handle off water pump thought to be infected. was actually river water
Joseph lister
father of aseptic surgery. disinfection of instruments. use of phenol on dressings, wounds, and in air.
Robert koch
proved that bacillus anthracis caused anthrax
koch's postulates
a particular disease is caused by a particular microorganism

the suspected pathogen must be present in every case, suspected must be isolated from disease host and grown in pure culture, organisms in culture music cause the same disease in healthy test host, same organism must be consistently reisolated from host
Koch's students
Walter Hesse- found use of agar when other things were too poor in nutrents.
Richard petri- the petri dish
Edward jenner
vaccinations. developed smallpox vaccine from cowpox, based on variolation
Louis Pasteur vaccines
fowl cholera, anthrax, rabies
chemotherapy
use of chemicals for treatment; using chemical agents to kill infetion in the body
Paul Ehrlich magic bullet
chemotherapy- syphallis using salvarsan
Gerhard Domagk
chemotherapy- prontosil rubrum. in vivo- converts to sulfanilamide. in vitro- nothing
Alexander flemming
discovered effects of penecillium notatum and its extracts on the growth of bacteria
Howard Florey and Ernst chain
development of penicillin as an antibiotic
virus
obligate intracellular parasites
What other structure, besides nucleic acid and protein coat, can a virus have?
animal viruses can have an envelope
minimum components of a virus
nucleic acid (either DNA or RNA, but not both)
Protein coat (capsid)
What is a characteristic of life?
Having DNA or RNA
Influenza
acellular
Viral disease
Has three types (A,B,C), and each has various strains within each.
A respiratory illness
Symptoms: coughing, sneezing, congestion, fever, body aches
Common Cold
acellular
Viral infection
200 different viruses (Rhinoviruses, adenviruses, coronaviruses)
Symptoms: coughing, sneezing, congestion
Rabies
acellular
Viral infection
hydrophobia
An infection of the nerves
A bullet shaped virus
AIDS
acellular
viral infection
acquired immune deficiency syndrome
HIV-human immunodeficiency virus)---transmitted through blood
viroid
acellular
plant pathogen
"naked" infectious RNA (no capsid)
prions
acellular
"naked" infectious protein
infect nervous tissue and damage brain
transmissible spongioform encephalopathies
acellular
prion
brain looks like a sponge
kuru, scrapie (goats and sheep), creutzfeldt-Jakob Disease(variant of mad cow), bovine spongiform encephalopathy(mad cow disease), chronic wasting disease
Syphilis Stages
primary: chancre-hard sore near infection
secondary: rash on skin and mucous membranes-fever, hair loss
latent: asymptomatic-transmitted from mom to baby, who is in tertiary)
tertiary: gummas (rubbery mass of tissue)

treatment can only be given during first three stages, last is death.
Eukaryotes
fungi, algae, protozoa, helminths(roundworms, flatworms, tapeworms, flukes)
irregular shapes (morphology)
pleomorphic rods: corynebacterium ditheria (chinese lettering, gray pseudomembrane)
highly pleomorphic: mycoplasmic pneumonia: no cell wall, causes primary atypical pneumonnia
Prokaryotes
bacteria
cell morphologies and arrangements
coccus (pl. cocci): staphylococci (clusters), streptococci (chains), diploccoci (pairs)
Bacillus (pl. bassili): Bacillus spp. and Clostridium spp. produce endospores(cannot be killed by disinfectant)
Examples of coccus
staphylococcus aureus: can cause URI, TSS, otitis media, MRSA,
staphylococcus epidermidis: can cause URI, sometimes boils, abcesses
Streptococcus pyogenes: can cause rheumatic fever which can cause rheumatic heart disease, and then scarlet fever
Enterococcus faecalis: normal flora of the colon
Streptococcus pneumonia: causes pneumonia, meningitis, otitis media
Neisseria gonorrhoeae: pelvic inflammatory disease
Neisseria meningitidis: causes menigitis, URI, and pneumonia
Examples of bacillus
B. Anthracis: anthrax
Clostridium tetani: tantanus (lock jaw)
C. botulinum: botulism poisoning, infant botulism (floppy baby syndrome)
C. perfringens: perfringens food pooisoning, and gas gangrene (wound infection)
C. difficile: nosocomial infection (from hospital)
Escherichia coli: normal microbiota of colon/large intestine, can cause food borne infection #1 cause of UTI
Pseudomonas aeruginosa: nosocomial infection, noted for infecting burns. naturally resistant to antiseptics.
Shigella spp.: 4 species that cause shigellos-bacillary dissentery
Salmonella spp.: #1 food borne infection. causes salmonellosis. NOT salmonella poisoning.
short rods (morphology)
coccobacilli: Haemophilus influenza type b (Hib)
comma shaped (curved) rod bacteria
Vibrio cholera (rice water stool)
Spirillium examples
Campylobacter jejuni: causes FBI
Helicibacter pylori: ulcers
spirochetes examples
Treponema pallidum: syphilis
simple staining
bacterial cell walls and cell membranes are negatively charged at neutral pH. A basic dye is used which has a positive charge
Negative Stain
color background, does not stain cell
Differential stain
Gram stain, acid-fast stain, structural stain
Acid-fast stain
acid-fast bacteria: mycobacterium tuberculosis, mycobacterium leprae, mycobacterium bovis, M. kansasii, M. snegmatis, M. avium-intracellulare complex, Norcardia spp.
Structural stain
endospore, capsule, flagella