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192 Cards in this Set

  • Front
  • Back
Primary sources of microorganisms in foods
Inhabitants

Transients
Primary sources of microorganisms in foods
Soil and Water
Plants and Plant Products
Food Utensils
Gastrointestinal Tract
Food Handlers
Animal Feeds
Animal Hides
Air and Dust
Factors associated with food spoilage
Color defects
Undesirable changes in texture
Off-odor development
Off-flavor development
Slime development
Any other changes making food undesirable
Spoilage is typically from what?
Microbial action
Initial microflora on food is what?
Highly variable
What happens to microbial diversity as spoilage progresses and what influences this?
Diversity become less.

Influenced by storage and packaging conditions.
Spoilage characteristics at ~10^6-7
Defects may be noticed
Spoilage characteristics at >=10^7
Off odors noticed
Spoilage characteristics at >=10^8
Tacky/slime formation starts
Types of microbial changes
Proteolysis/Putrefaction
Lipolysis
Fermentative Spoilage
What is Proteolysis/Putrefaction?
Aerobic/Anaerobic decomposition of proteins

Decarboxylation and deamination can occur
How does Proteolysis/Putrefaction occur?
Proteins are decomposed by proteases to amino acids, peptides, ammonia, and H2S
What is Lipolysis and how does it occur?
Usually a later type of spoilage where lipids decompose

Fats are degraded by lipases to fatty acids and glycerol
What is fermentative spoilage and how does it occur?
Desirable or undesirable fermentation

CHO (carbs) are broken down to lactic, btyric, propionic acids, CO2, alcohols, etc
When does Pseudomonas usually produce proteases and lipases?
Typically during late log phase around >= 10^8 cfu/cm2.
In what order does microbial enzymatic use usually occur?
Carbohydrates then amino acids/proteins then fatty acids/lipids
What odors and flavors occur due to microbial enzymatic activities and why?
Sour, cheesy, acid due to short chain fatty acids and amines.
What types of microbes are usually responsible for fermentation?
Usually bacteria and yeasts
What are some Foodborne Bacterial Pathogens?
Arcobacter
Bacillus - anthracis, cereus
Campylobacter
Clostridium - botulinum, perfrigens
Escherichia coli [Enterohemorrhagic, Enteroinvasive, Enteropathgenic, Enterotoxigenic]
Listeria monocytogenes
Salmonella
Shigella
Staphylococcus aureus
Vibrio - cholerae, parahaemolyticus, vulnificus
Yersinia enterocolitica
What are some Foodborne Viral Pathogens?
Hepatitis A

Norwalk and Norwalk-like
What are some Foodborn Parasitic Pathogens?
Cryptosporidium parvum
Cyclospora cayetanensis
Entamoeba histolytica
Giardia lamblia
Toxoplasma gondii
What are some other microbial-related illnesses?
Histamine poisoning

Toxogenic phytoplanktons - Shellfish and Ciguatera poisoning
What are the factors affecting microbial growth and survival?
Water content
Environmental temperature
pH
Oxidation-Reduction potential
Nutrients
Presence/Absence of antimicrobials
Other microflora
Processing treatment
What criteria is used to determine bacterial taxonomy?
Phenotypic, Genetic (16S RNA/DNA), Phylogenetic
What are the uses of fungi in foods?
Products - soy sauce, beer, wine, breads, some cheeses, etc

Other - production of organic acid
What are the bad aspects of fungi?
Rot crops
Clogs production lines
Scum
Production of off-odors or flavors
Mycotoxins
What are common characteristics of fungi?
Eukaryotic
Heterotrophic - Excrete extracellular enzymes, Degrade substrates, Absorb dissolved nutrients
Can be filamentous
Cell wall of Cellulose and/or Chitin
Reproduction - sexual & asexual, asexual only, uncertain
No chlorophyll
No means of locomotion
What are some ways to preserve food?
Low/Reduced temperature
High temperature
Antimicrobials (preservatives)
Radiation
Modified atmospheres
Drying
High pressure processing
Pulsed electric fields
Aseptic packaging
Combination Hurdles
What are the three basic types of factors affecting microbial growth?
Intrinsic Parameters that are inherent to plants and animals, which can influence microbial activities
Extrinsic Parameters
Hurdle Technology
What are the stages of microbial growth?
Lag, Log, Stationary, Death
What occurs during the lag phase?
Cells induce or repress enzyme synthesis and activity
Initiate chromosomes and plasmid replication
If they are spores they differentiate into vegetative cells
What does the length of the lag phase depend on?
Temperature
Inoculum size
Physiological state of the organism

Also actively growing cells transferred to new environment have shorter lags

The environment can be manipulated to extend lag
What occurs in the Log (Exponential) phase?
Bacterial cells reproduce by binary fission
What is the number of organisms at any time during the log phase directly proportional to?
Initial number of microorganisms
What is the temperature effect during the log phase?
A decrease of 10 deg C will decrease the growth rate by 2-fold
What conditions should be considered in regards to temperature?
Initial load
Size
Time & temperature of storage, stock rotation
Temperature constancy
Packaging - helps keep product clean and dry; may insulate
How do low temperatures affect microorganisms?
Primarily restrict growth so not effective for bacteria or fungal population but some eukaryotic organisms susceptible
What is the optimum temperature range for thermophiles?
55-75 C
What is the minimum temperature range for Thermophiles?
40-45 C
What is the optimum temperature range for Mesophiles?
30-45 C
What is the optimum temperature range for Psychrotrophs?
25-30 C
What is the optimum temperature range for Psychrophiles?
12-15 C
What is the minimum temperature range for Mesophiles?
5-15 C
What is the minimum temperature range for Psychrotrophs?
-5 - +5 C
What is the minimum temperature range for Psychrophiles?
-5 - +5 C
What is the maximum temperature range for Thermophiles?
60-90 C
What is the maximum temperature range for Mesophiles?
35-47 C
What is the maximum temperature range for Psychrotrophs?
30-35 C
What is the maximum temperature range for Psychrophiles?
15-20 C
What effect does freezing have as a process in relation to microorganisms?
Higher organisms like parasites are sensitive while spores are resistant.

Toxins C. botulinum and S. aureus are stable

Gram positives are usually more resistant than gram negatives
How problematic are pathogens in frozen foods?
Pathogen problems are rare as most frozen food is cooked prior to consumption
What are the factors affecting heat resistance?
Spores (inherent resistance)
Cell type and number
Growth conditions
-Stage of growth
-Growth temperature
-Growth medium
Heating environment
-ph (Most Important >8 & <6)
-Moist heat more effective
-Salts
-Sucrose
-Fat
-Protein
What does the time/temperature relationship depend on?
Type of food
Quantity
Liquid/Solid
What is the typical cooking temperature?
160F (71 C)
What is the typical warming temperature?
140F (60 C)
What does blanching do to microbes?
Kills a sizable portion of the population, but this is not the primary purpose of blanching
What is pasteurization?
Heating sufficient to destroy vegetative forms of pathogens of concern.

Destroys most or all of the vegetative, spoilage microorganisms

Usually used in combination with additional factors like refrigeration
What is commercial sterility?
Thermal treatment that eliminates all microorganisms except thermophilic spores
What pH are low acid foods and what is special about them?
> 4.6

Must be canned using steam under pressure for sufficient time
What pH are Acid foods?
4.6 and below
Where is the Commercial Sterility of Thermally Processed Foods defined?
Code of Federal Regulations
What is the D-value?
A measure of the heat resistance of a microorganism at a specific temperature.

Time in minutes required to kill 90% (1 log) of population.

Size of initial load important.

Pasteurization based on 12D Concept
What is the Z-value?
Thermal death curve - relative heat resistance of a microbe at different temperatures.

The temperature required for a reduction of the D-value by 1 log.
Gr- rods; aerobic; related to Pseudomonas; plant inhabitants
Acidovorax
G- rods; strict aerobes; common in soils and waters; common on fresh refrigerated food products
Acinetobacter
G- rods; aquatic; gas produce from fermented sugars
Aeromonas
G- rods; Produce alkaline products from sugars; widely distributed in nature (e.g., compose material)
Alcaligenes
Gr+ rods; thermoacidophilic; sporeformers; related to Bacillus species
Alicyclobacillus
G- rods; strict aerobes; marine inhabitants; found on seafood; require seawater salinity for growth
Alteromonas
G- curved rods; similar to Campylobacter except they grow at 15C and are aerotolerant; found on poultry, pork and some other animal
products
Arcobacter
G+ sporeforming rods; aerobic; most species are mesophilic; common in soil and water
Bacillus
G+ sporeforming rods; common in soil, water, dust and on plants; formerly classified as Bacillus
Brevibacillus
G+ rods; closely related to Lactobacillus and Listeria; also share characteristics with Microbacterium; common on refrigerated processed meat and seafood products packaged in gas-impermeable packages
Brochothrix
G- rods; formerly classed as Pseudomonas; found on plants and in raw milk; associated with cystic fibrosis patients
Burkholderia
G- spiral-shape rods; mesophilic;
microaerophilic to anaerobic; found on poultry and in raw milk
Campylobacter
G+ rods; previously classified as
lactobacilli; heterofermentative; can grow at 0C; found on vacuum-package meats, fish, poultry
Carnobacterium
G- rods; mesophilic; found on vegetables and fresh meats
Citrobacter
G+ sporeforming rods; anaerobic; most are mesophilic; widely distributed
Clostridium
G+ rods; most are mesophilic, some
psychrotrophs; can spoil meat and vegetable products
Corynebacterium
G- rods; facultative anaerobic; mesophilic; yields positive coliform test; generally not adapted to the gastrointestinal tract
Enterobacter
G+- cocci; formerly known as “fecal
streptococci”; can be found in fecal material
Enterococcus
G- rods; common on plants
Erwinia
G- rods; facultative anaerobic; mesophilic; common in fecal material; yields positive coliform and fecal coliform tests
Escherichia
G- rods; formerly classed as Pseudomonas; found on plants and in raw milk; associated with cystic fibrosis patients
Burkholderia
G- spiral-shape rods; mesophilic;
microaerophilic to anaerobic; found on poultry and in raw milk
Campylobacter
G+ rods; previously classified as
lactobacilli; heterofermentative; can grow at 0C; found on vacuum-package meats, fish, poultry
Carnobacterium
G- rods; mesophilic; found on vegetables and fresh meats
Citrobacter
G+ sporeforming rods; anaerobic; most are mesophilic; widely distributed
Clostridium
G+ rods; most are mesophilic, some
psychrotrophs; can spoil meat and vegetable products
Corynebacterium
G- rods; facultative anaerobic; mesophilic; yields positive coliform test; generally not adapted to the gastrointestinal tract
Enterobacter
G+- cocci; formerly known as “fecal
streptococci”; can be found in fecal material
Enterococcus
G- rods; common on plants
Erwinia
G- rods; facultative anaerobic; mesophilic; common in fecal material; yields positive coliform and fecal coliform tests
Escherichia
G- rods; found on plants; can contribute to spoilage of refrigerated meats and vegetables
Flavobacterium
G- rods; can contribute to spoilage of refrigerated meats and vegetables
Hafnia
G+ cocci; members were originally Micrococcus species
Kocuria
G+ rods; “lactic acid bacteria”; those in foods are typically microaerophilic; common on vegetables and in dairy products and refrigerated vacuum-packaged meats
Lactobacillus
G+ cocci; “lactic acid bacteria”; produce lactic acid as primarily end product of fermentation; formerly in the genus Streptococcus
Lactococcus
G+ cocci; heterofermentative; “lactic acid bacteria”
Leuconostoc
G+ rods; commonly found in the environment; L. monocytogenes can grow at refrigeration temperatures
Listeria
G+ coccus; can grow in presence of high salt levels
Micrococcus
G+ rods; sporeformer; some species related to either the Bacillus or Clostridium species
Paenibacillus
G- rods; common on/in plants, seeds, soil, water and humans; new species composed of former Erwinia and Enterobacter species
Pantoea
G+ cocci; “lactic acid bacteria”;
homofermentative; they can grow in 18% salt
Pediococcus
G - rods; facultative anaerobic; mesophilic; common in the intestinal tract; swarming growth of agar plates; common on vegetable and meat products; can contribute to product spoilage
Proteus
G- rods; largest genus of bacteria found on fresh foods; common in/on soil, water, vegetables, meat, poultry, seafood; aerobic; many species can grow at refrigeration temperatures
Pseudomonas
G- rods; species were once included in Acinetobacter or Moraxella species; aerobic; can grow in 6.5% salt; found on meats, poultry, fish and in water
Psychrobacter
G- rods; facultative anaerobic; mesophilic
Salmonella
G- rods; can contribute to spoilage of refrigerated vegetables and meats
Serratia
G- straight or curved rods; associated with aquatic or marine environments
Shewanella
G- rods; facultative anaerobic; mesophilic
Shigella
G+ cocci; salt tolerant; mesophilic
Staphylococcus
Gr+ rods; anaerobic; sporeformer; related to Clostridium; can cause problems in canning
Thermoanaerobacterium
G+ cocci; composed of former group N lactococci; can grow at 10C but not at 45C
Vagococcus
G- straight or curved rods; requires low levels of salt; common in marine environments
Vibrio
G- rods; closely related to Leuconostoc; species were former Leuconostoc species; heterofermentative
Weissella
G- rods; facultative anaerobic; mesophilic
Yersinia
Common in dairy products; 1 species can grow in 24% salt and at an Aw as low as 0.65; forms slime on wieners; grows in brine and on cheeses; spoils orange juice concentrate and yogurt
Debaryomyces
Yeast found on figs, tomatoes, strawberries, citrus fruits, and cacao beans
Hanseniaspora
Yeast common on a variety of foods
Issatchenkia
Yeast common in dairy products; can spoil fruits and dairy product
Kluyveromyces
Large genus; can spoil pickles and sauerkraut; common on fresh fish and shrimp; important in making some indigenous foods in parts of the world
Pichia
bakers’, brewers’, wine and champagne yeasts; also found in kefir grains and wide variety of foods
Saccharomyces - S. cerevisiae
Yeast... some are osmophilic and resistant to some chemical
preservatives
Schizosaccharomyces
Strong fermenters of sugars; Z. rouxii can grow at aw of 0.62; One can grow at pH 1.8; Some involved in miso and shoyu fermentations; some spoil mayonnaise and salad dressing
Zygosaccharomyces
Common yeast, can grow at pH 1.8; can spoil beer, wine, soft drinks, and pickles
Brettanomyces - B. intermedius
Yeast common in ground beef and poultry and a variety of foods; some
may play a role in fermentation of cacao beans and other products
Candida
Yeast found on plants, soil, fruits, fish, shrimp, fresh ground beef
Cryptococcus
Produce pink/red pigments; some are psychrotrophic; found on
butter, fresh poultry, shrimp, fish, and beef
Rhodotorula
Involved in cacao bean fermentation; found on fresh shrimp, ground beef, poultry, frozen lamb, and other foods
Trichosporon
5 important protozoa in the US
1. Cryptosporidium parvum
2. Cyclospora cayetanensis
3. Entamoeba histolytica
4. Giardia lamblia
5. Toxoplasma gondii
conditions referred to as “whiskers” of beef or “black spot” on frozen mutton; other species found on a variety of foods
Mucor
“bread mold”; produces watery, soft rot on variety of fruits; “black
spot” on beef and frozen mutton; found on bacon and refrigerated meats; one species important in fermentation of tempeh, oncom, and bonkrek
Rhizopus
1 species; found on refrigerated beef; “whiskers” on beef
Thamnidium
Produces heat resistant ascospores; can tolerate low oxidation-reduction potential
Byssochlamys
cause brown to black rots of some Fruits and stem-end rot and black rot of citrus fruits; field fungi that can grow on wheat; has been found on red meats; some species can produce mycotoxins
Alternaria
black rot of some fruit; some species can spoil oils (e.g., palm,
corn, peanut); some species important in soy fermentation; 2 species produce mycotoxins
Aspergillus
Found on shrimp; “black spot” of long-term stored beef; common on vegetables and fruits
Aureobasidium
Gray mold rot on fruits
Botrytis
“black spot” on beef and frozen mutton; some spoil butter and margarine; some rot fruits; field fungi that grow on barley and wheat
Cladosporium
Brown rot of citrus fruits and pineapples and soft rot of figs; field
fungi on barley and wheat; some species can produce mycotoxins
Fusarium
“machinery mold”; especially a problem in tomato processing plants; can spoil some fruits and some dairy products; common on a variety of fruits and vegetables
Geotrichum
Brown rot of some fruits
Monilia
Blue and green mold rots of fruits; P. roqueforti produces blue cheese; some species can produce mycotoxins
Penicillium
1 species; pink rot of fruits; common on variety of grains; some can produce mycotoxins
Trichothecium
Can grow at Aw of 0.69
Wallemia
Minimum growth Aw is 0.61; maximum Aw is <0.97; is somewhat
heat resistance; spoils licorice, prunes, chocolate syrup
Xeromyces
What is the F0?
The number of minutes required to destroy a specific number of spores at 121.1C (250F) when z is 10C (18F)

It varies for substrate and microorganism
What must be used for controlling microorganisms with temperature?
Proper sanitation
Proper pH
Proper time/temperature relationship
Proper sealing
Proper container integrity
What is the water activity (Aw)?
Water needed for microbial growth

P/P0
Vapor pressure of food / Vapor pressure of pure water at same temp.
What occurs at low water activity?
If low enough, lag phase lasts indefinitely and growth stops

microbes are also more resistant to heat effects
What is a Xerophile?
Grows at Aw < 0.85
What company plead guilty to salmonella charges?
Cadbury
How do you use O-R potential to inhibit aerobic microorganisms?
Use facultative microorganisms
What can act as a selective agent for microorganisms in foods?
Ox-red potential
How is ethylene oxide used?
Can penetrate most organic material.

Used in whole or ground spices except those containing salt.
How is ozone used?
Unstable, powerful oxidizing agent

Used in water treatment and bottle sterilization
What is propylene oxide used in?
Dried foods, cocoa, gums, etc
What is altering the atmosphere good for?
Large masses of food, esp if heated
What type are most foodborne pathogens?
Facultative anerobes or anerobic
What pH range do most bacteria prefer?
6.5-7.4
What pH type do most molds and yeast grow in?
Acidic

Main cause of spoilage is these foods
Most meats are at what pH?
5.6 and above
Most vegetables are higher or lower in pH than fruits?
Higher

So more susceptible to bacteria than mold
Buffering is used more in what?
Meats
What does pH have an effect on?
Enzyme function
Cell transport of nutrients
Younger more than older/resting cells
Temperature and salt concentration
Lag phase outside optimum range
What are the requirements for used of chemical preservatives?
Economical
Only if other methods inadequate or unavailable
Extend storage life
Does not lower food quality
Readily soluble
Antimicrobial properties over the food's pH range
Safe at needed levels
Readily identified by chemical analysts
Does not retard digestive enyzmes
Does not yield more toxic compounds
Easily controlled and uniformly distributed in food
Wide antimicrobial spectrum
Where are organic acids and esters found?
Fruits (citric, sorbic, benzoic, etc)
Meats (lactic)
Metabolic products of m/o (fermented foods)
How do organic acids and esters work?
Undissociated form passes through cell membrane then ionizes inside cell interfering with permeability of cell membrane
Shorter chain organic acids and esters inhibit what?
Both gram - and +, passing through cell membrane easily
Long chain organic acids inhibit what?
Primarily gram positive

Difficult to pass through gram negative
What bonus is associated with organic acids and esters?
Can potentiate antimicrobial effect of nitrite and sufite
What pH do organic acids and esters form?
At lower pH generally < 5,5
What are some problems with organic acids and esters?
Less effective if initial cell # is high
Some m/o can use as energy source
Resistance varies in strains
What are strong acids widely used in?
Just carbonated beverages
What is acetic acid good for?
It is effective and widely used
What is benzoic acid good for?
Antimycotic activity
What is citric acid good for?
It has moderate antimicrobial activity
What are p-hydroxy benzoic acids (parabens) good for?
Effective over a wider pH range
What is propionic acid good for?
A mold inhibitor that is ineffective on yeast

great for baking
What is sorbic acid good for?
Effective against catalase positive bacteria, yeasts and molds
(good for fermentations)

And good against germinating endospores, preventing outgrowth
How is NaCl used?
Primarily decreases Aw

Used for creating selective environment for lactic acid bacteria

Inhibit botulinum
What is sulfur dioxide used for?
Soft drinks, juices, wines, sausages, pickles

Levels controlled due to hypersensitivity problems
What are the types of curing salts?
NaCl, NaNO3, NaNO2, KNO3, KNO2
How does nitrite work?
Prevents outgrowth of spores
What is the issue with antimicrobial plant substances like eugenol, cinnamic aldehyde, and caffeine?
The are weak and using just one results in off-flavor issues.
What is lactoperoxidase?
Antimicrobial in milk against sensitive gram negatives
What is Nisin?
Heat stable protein (121C) produced by Lactococcus lactis that is inhibitory toward many gram positives by adsorption to the cell membrane and pore formation (inactivation of sulfhydral groups) causing disruption of proton motive force. Used in processed cheeses.
What is the primary action of antimicrobials?
To slow growth
How are antibiotics used in food?
Not added to food directly but in animal production as a feed supplement and as sub theraputic treatment
What types of ionizing radiation are useful? How?
Gamma, x-rays, electron beams

Directly damages genetic material or indirectly through free radicals or peroxide formation
What types of ionizing radiation are not useful and why?
Alpha particles (poor penetration)
Neutrons (induce radioactivity)