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185 Cards in this Set

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Aerobic gram positive cocci
Enterococcus, Staphylcoccus, Streptococcus, Micrococcus
Anaerobic gram positive cocci
Peptococcus, Peptostreptococcus
polymicrobial infections
--2+ bacterial pathogens involved in disease process
--could be obligate anaerobes or facultative anaerobes (most contain both)
--cannot initiate or sustain infection by themselves
coagulase-positive staphylococcus
staphylcoccus aureus
staphylcoccus hyicus
staphylcoccus intermedius
--able to coagulate rabbit plasma
--antiphagocytic carb capsule
cellular arrangement of staphylococcus
grapelike clusters
cellular arrangement of streptococcus
chains
(streptococcus pneumoniae arranged in pairs)
Staphylcoccus hemolysis
--coagulase pos have alpha, beta, delta, and epsilon toxins
--complete if coagulase pos
--no hemolysis if coagulase neg
--S. aureus has double zone
Staphylococcus aureus diseases
Human: foodborne intoxification, skin infections
bovine: mastitis
canine: pyodermas (10% cases)
Staphylcoccus intermedius diseases
causes 90% canine pyodermas cases
Streptococcus agalactiae disease
bovine: mastitis
streptococcus equi subspecies equi disease
equine: strangles
streptococcus pneumoniae disease
human: pneumococcal pneumoniae
streptococcus pyogenes diseases
human: glomerulonephritis, rheumatic fever, scarlet fever, strep throat
streptococcus hemoylsis
can be either complete, incomplete, or nonexistant
hemolytic colonies considered pathogenic
Lancefield classifications
A-H; K
based on precipitation test of extractable group-specific carb cell wall antigens (C carbs)
Lancefield group A
streptococcus pyogenes
hyaluronic acid capsule (nonantigenic)
Lancefield group B
streptococcus agalactiae
Lancefield group C
streptococcus equi subspecies equi
hyaluronic acid capsule (nonantigenic)
Lancefield group D
enterococcus species
M-proteins
Streptococcus equi subspecies equi
antiphagocytic virulence factor that extends through capsule to assist attachment to larynx
extracellular virulence factors of streptococcus
hyaluronidase, streptodornase, streptokinase, streptolysins O and S
hyaluronidase
extracellular virulence factor
hydrolyzes hyaluronic acid
streptococci
streptodornases A,B,C,D
extracellular virulence factor
deoxyribonuclease; hydrolyzes DNA; streptococci
streptokinases A and B
streptococci extracellular virulence factor
lyse fibrin by catalyzing conversion of plasminogen to plasmin
promotes blood clot lysis
streptolysin S
streptococci extracellular virulence factor
nonantigenic; oxygen-stable; lyses macrophages, neutrophils, platelets
streptolysin O
streptococci extracellular virulence factor; antigenic and oxygen-labile; lyses macrophages, neutrophils, platelets
Glomerulonephritis
Streptococcus pyogenes infection
based on M-protein antigens
knocks out kidneys
Rheumatic fever
streptococcus pyogenes infection
assoc. with >50 serovars
antibodies react against sarcolema and produce permanent lesions on the heart; life-threatening disease
Baitracin susceptibility test
streptococcus pyogenes are susceptible
Dick test
in vivo toxin neutralization test
erythrogenic toxins cause scarlet fever
intradermal inoculation--red skin rash shows susceptibility
Optochin susceptibility test
Streptococcus pneumoniae is susceptible
Quelling test
anticapsular IgM antibodies attach to surface of S. pneumoniae capsule, allowing us to visualize it
Progression of pneumoniae
congestive stage, red hepatization stage, gray hepatization stage, resolution stage
purpore hemorrhagia
post-infection immune complex disease; subcutaneous edema on muzzle and limbs; petechial and ecchymotic hemorrhages
aerobic gram positive bacilli
arcanobacterium, corynebacterium, dermatophilus, erysipelothrix, listeria, rhodococcus
arcanobacterium pyogenes disease
suppurative lesions and abscesses in cattle and sheept
corynebacterium diphtheriae disease
diphtheria (humans)
corynebacterium pseudotuberculosis disease
lyphadenitis in sheep
corynebacterium renale disease
pyelonephritis in cattle
dermatophilus congolensis disease
dermotphilos (humans, cattle, horse, sheep)
erysipelothrix rhusiopathiae disease
humans: erysipeloid (skin infection)
pigs: erysipelas (acute septicemia)
listeria monocytogenes disease
foodborne infections (human, cattle, sheep)
rhodococcus equi disease
foal pneumonia, found in AIDS patients
ovine infective bulbar necrosis
A. pyrogenes and F. necrophorum; in medial digits of hind legs of pregnant ewes; produes leukotoxin
dipheroid cellular appearance
cells divide longitudinally
dumbbell shape
produce cells via binary fission that are V,L, or Y shaped
Babes-Ernst granules
assoc. with diphtheria
made of inorganic phosphate (volutin)
stain with Albert's stain
cytoplasmic granule
diphtheria toxin prototoxin
inactive prototoxin = MW 62,000
1 A subunit (24,000) + 1 B subunit (38,000)
B binds to cell and A enters
action of diphtheria toxin
inhibits polypeptide chain elongatoin by catalyzing inactivation of elongation factor 2->stops protein synthesis
DPT vaccine
D = diphtheria toxoid
P = bordetella perstussis
T = tetanus bacteria
Dermatophilus congolensis life cycle
nonmotile bacilli = tissue phae
motile zoospores = infective phase; peritrichous flagella
Dermatophilus ovine diseases
"lumpy wool" (wooly area affected), "strawberry footrot" (distal area affected)
erysipelothrix diseases
E. rhusiophatiae causes Erysipeloid in humans and erysipelas in pigs
Erysipelothrix rhusiphatiae cellular characteristics
gram pos bacillus
facultative anaerobe
complete hemolysis
highly pleiomorphic
porcine erysipelas
swine are carriers--natural GI flora
2 mos-1 yr most affected
septicemias, cutaneous lesions
can affect joints and endocardium
contributing factors to listeriosis in ruminants
poor quality silage
older silage increases listeria concentration
alkaline pH
Listeria monocytogenes motility
temp-dependent motility
motile with peritrichous flagella at 4-25C
nonmotile at 37C (body temp)
Listeria monocytogenes cellular characteristics
gram pos bacillus
facultative anaerobe
complete hemolysis
catalase positive
facultative intracellular pathogen
Bovine listeria monocytogenes infections
asymptomiatic enteric infections
visceral infections with septicemia
abortions
neural listeriosis (most common)
Rhodococcus equi diseases
Equine: foal pneumonia
human and other animals: opportunisitic infections
Mycobacterium cellular characteristics
gram pos acid-fast bacilli
facultative intracellular pathogens
obligate aerobes
high lipid content in cell wall
stain only with Ziehl-Neelsen
culture on Lowenstein-Jensen, not blood, agar
Nocardia cellular characteristics
gram pos partial acid-fast bacilli
stain with Gram and Kinyoun stains
obligate aerobes
Lowenstein-Jensen agar
Able to culture most mycobacterium species
aerobic conditions
Myobacterium avium serovars
1-3: causes mycobacteriosis and tuberculosis in animals, birds, porcine
4+: causes mycobacterium in animals and humans
Runyoun Group I
Photochromogens
Nonpigmented at dark incubation
yellow-orange at light incubation
slow growth
Runyoun Group II
Scotochromogenes
Yellow-orange at dark incubation
Yellow-orange at light incubation
slow growth
Runyoun Group III
Nonchromogens
Nonpigmented at dark incubation
Nonpigmented at light incubation
Slow growth
Includes pathogens: M. avium, M. bovis, M. leprae, M. tuberculosis
Runyoun Group IV
Rapid Growers
Nonpigmented at dark incubation
Nonpigmented at light incubation
Rapid growth
Myobacterium avium serovars 1-3
Cause porcine tuberculosis
Called avian tubercle bacilli
Mycobacterium avium serovars 4 and above
Soil saprophytes
Can cause mycobacteriosis in humans, cattle, and swine
M. bovis and M. tuberculosis
Called mammalian tubercle bacilli
Cattle are primary host of M. bovis
HUmans are primary host of M. tuberculosis
Tuberculin test
Mammalian tuberculin
intradermal inoculation, read 36-48 hrs later
positive delayed hypersensitivity rxn: redness, bump
this means you've been exposed
Mammalian PPD (purified protein derivative)
Tells you that you've been exposed to mycobacterium and tuberculosis
doesn't tell you if you have an active infection
BCG vaccine
Bacillus of Calmetter and Geruin vaccine
Live attenuated M. bovis strain
Induces cell-mediated immunity
After this vaccine, TB test will always come back positive
avian tubercle bacilli
Mycobacterium avium serovars 1-3
cause of porcine tuberculosis
mammalian tubercle bacilli
Mycobacterium bovis and Mycobacterium tuberculosis
cause of TB in human and bovine
Lepromatous leprosy
Mycobacterium leprae
when individual has weak cell-mediated response
marked deformitites in hands and feet
Tuberculoid leprosy
Mycobacterium leprae
when individual has strong cell-mediated response
localized skin lesions
Progression of bovine tuberculosis
lesions form at reticuloendothelial tissue
local acute suppurative inflammation
lymphocytes infilitrate-->macrophages become giant cells
coagulation necrosis and caseous necrosis
Diagnostic tests for bovine tuberculosis
Caudal fold test (intradermalninjection of M. bovis PPD)
Comparative cervical test (infect cattle with M. bovis PPD and B. avium PPD)
Nocardia asteroides
Opportunistic infections in animals and humans
chronic pyogranulomatous lesions
bovine mastitis, canine pulmonary nocardiosis
Bacillus anthracis
anthrax in humans and animals
Bacillus cereus
foodborne intoxification in humans
Clostridium botulinum
botulism (flaccid paralysis) in humans and some animals
Clostridium tetani
tetanus (tonic muscular contractions) in human, bovine, equine, porcine
Clostridium chauvoei
bovine blackleg
acute febrile disease with lameness, gas production
acute endocarditis
Clostridium perfringens
ovine entertoxemia (pulpy kidney; overeating disease)
human foodborne intoxifications
Gram positive sporeforming bacilli
Bacillus (facultative anaerobes) produce spores in aerobic conditions
Clostridium (obligate anerobes) produce spores in anaerobic conditions
virulence factors of Bacillus anthracis
D-Glu polypeptide capsule
exotoxin complex (Factors I, II, III)
virulence factors of Bacillus cereus
heat-labile enterotoxin (diarrhea)
emetic toxin (vomiting)
oval subterminal endospore
Bacillus anthracis susceptibility for common creatures
Humans: cutaneous anthrax infections, pulmonary anthrax infections
bovine/ovine: peracute, acute, or chronic infections
equine/swine: acute pharyngitis
feline/canine: rare occasion
Exotoxin complex of B. anthracis
Factor I = edema factor
Factor II = antiphagocytic protective antigen
Factor III = lethal factor
must have factor I and III associated with II for the biological activity
forms of human anthrax
cutaneous anthrax
systemic anthrax
pulmonary anthrax
transmission of C. botulinum
most common = intoxification of preformed botulinum toxin
rare = toxicoinfection where GI tract is infected and then toxin is released and absorbed
botulinum toxins
attach to presynaptic terminal of cholinergic nerves to block release of acetylcholine
protoxin-->holotoxin (1A + 5B)
types A-G; heat-labile
A,B,E,F,G on chromosome; C,D in lysogenic bacteriophages
botulinum toxin susceptibility
A,B,E,F = human
C,D = bovine
B = equine
D = ovine
transmission of C. tetani
toxicoinfection: infection, followed by intoxification
parental infections from wounds, puerperal infections postpartum, and via umbilical cord
tetanospasmin
prototoxin cleaved by proteases-->holotoxin
1A (50,000) + 5B (20,000 each)with disulfide links
fixed to ganglioside
blocks spinal inhibitory synapses
gamma-amino-butyric acid (pre-synaptic), glycine (post-synaptic inhibition)
neural spread of tetanospasmin
tetanospasmin transported on axons from peripheral nerves to CNS
localized ascending tetanus
vascular spread of tetanospasmin
blood transport
produced in head and progressively involves body
generalized descending tetanus
C. perfringens Type A antiseum
antibodies to alpha toxin
protects against Type A
C. perfringens Type B antiserum
antibodies to alpha, beta, epsilon toxins
protects against Types A,B,C,D
C. perfringens Type C antiserum
antibodies to alpha and beta toxins
protects against Types A and C
C. perfringens Type D antiserum
antibodies to alpha and epsilon toxins
protects against Types A and D
C. perfringens Type E antiserum
antibodies to alpha and iota toxins
protects against Types A and E
C. perfringens virulence factors
usually Type A toxin (alpha)
alpha, delta, kappa, lambda, mu, theta toxins
neuraminidase
extracellular enzymes and toxins
tetanus toxoid
formalin-inactivated tetanospasmin
100% immunity
good memory initiation (espeically with IgG)
Actinomyces bovis
actinomycosis (bovine)
proliferative osteomyelitis of mandible/maxilla
chronic granulomatous lesions in head soft tissues (sulfur granules)
Actinomyces israelii
actinomycosis (humans)
chronic granulomatous lesions
sulfur granules
Bifidobacterium, Eubacterium, Propionibacterium
anaerobic gram-pos bacilli
opporunistic pathogens of enteric
Actinomyces viscosus
canine pulmonary actinomycosis
Actinomyces
gram pos pleomorphic bacilli
obligate anaerobes
Equine Fistulous Withers and Poll Evil
Actinomyces bovis
Brucella abortus (main cause)
Brown-Breen stain
tissue stain used to visualize Actinomyces bovis
sulfur granules
in vivo granules of pale yellow color
bacterial colony in hyaline matrix
assoc. with A. bovis and A. israelii
Neisseria characteristics
aerobic gram neg cocci
found in pairs
cultured on chocolate agar under capnophilic conditions
Veillonella characteristics and infections
facultative anaerobic gram neg cocci
opportunistic infections with suppurative exudate & abscess formation
polymicrobial infections
BHI agar with 10% CO2, 10%H, 80% N
Neisseria gonorrhoeae
Gonorrhea (human)
STD
causes infertility
infection of mucous membranes of genitalia with suppurative exudate
Neisseria meningitidis
Meningococcal meningitis (human)
6-24 mos or 10-20 yrs old
meninges (& pharyngeal) lesions
Enterobacteriaceae pathogenic species causing enteric infections in humans
Escherichia
Salmonella
Shigella
Yersinia
Enterobacteriaceae pathogenic species causing enteric infections in humans and animals
Ercherichia
Salmonella
Enterobacteriaceae pathogenic species causing extraintestinal infections in humans and animals
Citrobacter, Enterobacter, *Escherichia, *Klebsiella, Morganella, Proteus, Providencia, Salmonella, Serratia, Yersinia
characteristics of family Enterobacteriaceae
Gram neg bacillus
Facultative anaerobes
Nitrate reductase positive
Catalase negative
Citrobacter characteristics
gram neg bacillus, facultative anerobic
extracellular pathogen
opportunistic extraintestinal infections; polymicrobial
Enterobacter characteristics
gram neg bacillus, facultative anaerobe
extracellular pathogen
opportunistic extraintestinal infections, polymicrobial
E. coli extraintestinal diseases
genital tract, mastitis, urinary tract, wounds, pneumonia (secondary infection)
Klebsiella extraintestinal infections
bovine mastitis
canine cystitis, mastitis, metritis
equine metritis
porcine mastitis
Morganella characteristics
gram neg bacillus, facultative anaerobe
extracellular pathogen
in wound and urinary tract infections
polymicrobial
Proteus characteristics
gram neg bacilli, facultative anaerobe
extracellular pathogen
opportunistic extraintestinal infection (common in UTI)
in pure or polymicrobial cultures
swarmer on blood agar
Providencia characteristics
gram neg bacillus, facultative anaerobe
extracellular pathogen
opportunistic extraintestinal infection
polymicrobial
Serratia characteristics
gram neg bacillus, facultative anaerobe
extracellular pathogen
opportunistic extraintestinal infection
polymicrobial
Lac operon
family Enterobacteriaceae
permease
beta-galactosidase
lactose utilization/metabolism
permease
outer membrane protein which actively transports lactose into cytoplasm
assoc. with lac operon
beta-galactosidase
cytoplasmic enzyme which hydrolyzes lactose to glucose and galactose
assoc. with lac operon
colibacillosis
enteric infections
Escherichia coli
animals and humans
E. coli serovars
enteropathogenic, enterohemorrhagic serotyped with O,K, H antigens
enteroinvasive serotyped with O, K antigens
enterotoxigenic serotyped with F antigens
Fimbrial antigens of enterotoxigenic E. coli
human: CFA I-IV
porcine: F4,F5,F6,F41
bovine/ovine: F5,F41
some E. coli cellular characteristics
hemolytic or not
peritrichous flagella (+/-)
lactose positive; A/A + gas
EMB agar
enterohemorrhagic E. coli
ileum (lower SI)
bloody diarrhea (O157:H7)
shigalike toxins I and II
low infective dose
enteroinvasive E. coli
like bacillary dysentery (blood and mucous in diarrhea)
nonmotile; LI and colon
shigalike toxins I and II
enteropathogenic E. coli
like enterocolitis of Salmonella
enterotoxins and shigalike toxins
ileum and LI
enterotoxigenic E. coli
upper small intestine
hypersecretion of water and electrolytes causes dehydration
virulence factors of enterotoxigenic E. coli
fimbrial antigens
heat-labile enterotoxin (holotoxin; 1A + 5B)(85,000)
heat-stable enterotoxins A/B (2,000)
hemolysin; lipopolysaccharide
biological activity of heat-labile enterotoxin of enterotoxigenic E. coli
B subunit binds to Gmi ganglioside to form pore
A subunit does ADP-ribosylation of GTP-binding protein, activating adenylate cyclase and increasing cAMP level
biological activity of heat-stabile enterotoxins of enterotoxigenic E. coli
nonantigenic polypeptides
A activates guanylate cyclase, increasing cGMP levels
B is antiabsorptive
tests to detect enterotoxigenic E. coli enterotoxins
heat-labile: ligated ileal loops of live rabbits; tissue culture methods with Y1 mouse adrenal cells or Chinese hamster ovary cells
heat-stable: infant mouse test
Salmonella typhi
typhoid (human)
septicemia with high fever and gastroenteritis
capsular Vi antigen
endotoxin, cytotoxin, enterotoxins
anaerogenic
Salmonella choleraesuis
porcine salmonellosis (also S. typhimurium)
anorexia, fever, mucohemorrhagic irritation, emaciation; black tarry diarrhea
Salmonella serovars
over 2500
all but 2 are motile
serotyped with O, H antigens
slide agglutination tests
identification of Salmonella serogroups A-I
Salmonella biochemical characteristics
gram neg bacilli
oxidase negative
K/A + gas + H2S
Salmonella enterocolitis
ileum and LI
infective dose = 10^8
invade intestinal mucosa, infect lymph nodes
black tarry diarrhea and hemorrhagic lymph nodes
bacteremia/septicemia
Salmonella typhimurium
equine enterocolitis
stress reduces min infective dose
high mortality
Salmonella abortusequi
equine abortion
Salmonella gallniarium & Salmonella pullorum
avian-adapted salmonella serovars
nonmotile
foul typhoid and pullorum disease
Shigella dysenteriae
Bacillary dysentery (human)
mannitol positive
low infective dose (200)
cecum and LI
diarrhea with blood and mucous; high fever; septicemia/endotoxemia
Shigella boydii, S. flexneri, S. sonnei
Diarrhea (human)
intestinal lesions
virulence factors of shigella dysenteriae
polysaccharide capsule
lipopolysaccharide
2 cytotoxins (shiga toxins I and II; verotoxins)
3 enterotoxins (heat-labile and heat-stable A/B)
neurotoxin
Yersinia enterocolitica
enteritis (human, animals)
facultative intracellular pathogen
motile with peritrichous flagella at room temp (nonmotile above)
diarrhea, lymphadenitis, septicemia
Yersinia pestis
plague (human)
flea (and rat) is biological vector
nonmotile
bubonic (endemic, buboes) or pneumonic (epidemic, septicemia)
Yersinia pseudotuberculosis
psuedotuberculosis (humans, animals)
facultative intracellular pathogen
motile with peritrichous flagella at room temp (nonmotile above)
caseous lesions in rats
virulence factors of Yersinia pestis
capsule (F1) and cell wall-associated (V/W) antigens
--heat-labile proteins
--temp-dependent (active at body temp)
lipopolysaccharide, catalse, murine toxin, determinant P, Pesticins I and II
Acinetobacter calcoaceticus
aerobic gram neg bacilli
extracellular pathogen with suppurative exudate
Aeromonas hydrophila
aerobic gram neg bacilli
motile; waterborne
mostly fish, reptile, amphibian pathogen
Alcaligenes characteristics
aerobic gram neg bacilli
enteric flora of vertebrates
mainly poultry pathogen
Francisella tularensis
tularemia (human); clinical infections (feline, canine)
Moraxella bovis
bovine infectious keratoconjunctivitis
Pseudomonas aeruginosa
aerobic gram neg bacillus
extracellular pathogens
often found in water
Taylorella equigenitalis
contagious metritis (equine)
mares: cervitis, endometritis, vaginitis)
stallions: asymptomatic
Bordetella bronchiseptica
pneumonia, tracheobronchitis (canine)
pneumonia (feline)
porcine atrophic rhinitis
affects apical and cardiac lobes of lungs
kennel cough
Bordetella pertussis
Pertussis (human)
severe tracheobronchitis
pathognomonic whooping cough
not on blood--grow on Bordet-Gengou agar
DPT vaccine
Brucella abortus
brucellosis (bovine, human)
smooth
abortion and orchitis
Brucella canis
brucellosis (canine, some subclinical infections in humans)
rough
abortions, stillbirths, epididymitis, orchitis
Brucella melitensis
brucellosis (ovine; human)
smooth
abortions, orchitis
Brucella ovis
ram epididymitis (ovine)
rough
abortions
Brucella suis
brucellosis (porcine; human)
smooth
abortions, stillbirths, orchitis
A & M antisera
agglutinate smooth species
monospecific B. abortus (A) and monospecific B. melitensis (M)
R antiserum
anti-rough antiserum
agglutinates rough species
Brucella characteristics
gram neg bacillus
obligate aerobes
facultative intracellular pathogens
some are capnophilic
standard tube agglutination test
serologic test for smooth Brucella species
IgM and IgG
quantiative test
slowest
Card test
serologic test for smooth Brucella species
IgG (acid pH inactivates IgM)
qualitative test
fastest
rose bengal dye (pink color)
2-mercaptoethanol test
serologic test for smooth Brucella species
IgG (2-mercaptoethanol inactivates IgM)
quantitative test
supplementary test
Rivanol test
serologic test for smooth Brucella species
IgG (rivanol inactivates IgM)
quantitative test
supplementary test
Brucella abortus strain 19
erythritol negative
non-capnophilic (doesn't require CO2 for growth)
polysaccharide B
Burkholderia mallei
equine glanders
obligate aerobe
cutaneous, nasal, and pulmonary glanders and ulcerative lymphadenitis
encapsulated nodules with yellow caseous purulent material
Mallein test
detects for Burkholderia mallei in horses
administered by intracutaneous or subcutaneous inoculation