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7 Cards in this Set

  • Front
  • Back

Adv of PCR

Highly sensitive


Specific


Rapid

Highly Sensitive PCR detect high or low quantities

Very low

Steps of PCR

-Denaturation


-Annealing


-Extension

At which temp the Primer extended w by

70 to 75 C



DNA Polymerase (Taq تاج)

At which temp denaturation

90-95

At which temp annealing

45 to 60


50 يعني

PCR Master mix composed of

Template DNA


2 Primers


4 Nucleotide


DNA Polymerase