Micro Lab_Mannitol Salt Agar/macconkey Agar/slide Aggultination

Front 1

Grows on a specific type of microbe

Back 1

selective

Front 2

able to distinguish two organisms on one plate

Back 2

differential

Front 3

Mannitol Salt Agar is selective and/or differential.

Back 3

selective and differential

Front 4

Which medium is selective for Staphylococcus?

Back 4

MSA

Front 5

What makes MSA differential?

Back 5

It can distinguish between pathogenic and nonpathogenic strains of Staphylococcus.

Front 6

What macromolecule is mannitol?

Back 6

carbohydrate

Front 7

What component of MSA makes it selective for Staphylococci?

Back 7

It is composed of 7.5% NaCl.

Front 8

For MSA, what color indicates acidity?

Back 8

Yellow

Front 9

For MSA, what color indicates a pH of 7.4 to 8.4?

Back 9

red

Front 10

For MSA, what color is indicative of basicity?

Back 10

Pink

Front 11

Pathogenic/Nonpathogenic species ferments mannitol.

Back 11

Pathogenic

Front 12

What is one example of a pathogenic species that ferments mannitol?

Back 12

S. aureus

Front 13

What does a pathogenic species do to MSA?

Back 13

It produces acid by dropping the pH. The media then turns yellow.

Front 14

Do nonpathogenic species grow on MSA?

Back 14

Yes, but they do not produce a color change.

Front 15

T/F Nonpathogenic species turn MSA red.

Back 15

False; nonpathogenic species grow on MSA, but do not produce a color change.

Front 16

It is recommended that an MSA plate be run alongside what?

Back 16

a growth control

Front 17

If poor growth or no growth on MSA plate indicates what about the organism?

Back 17

It is inhibited by NaCl and therefore, NOT staphylococcus.

Front 18

Good growth on an MSA plate indicates what?

Back 18

Organism is not inhibited by NaCl and therefore, is Staphylococcus.

Front 19

Yellow growth or halo on MSA indicates what?

Back 19

Organism produces acid from mannitol fermentation and is a possible pathogenic Staphylococcus aureus.

Front 20

Red growth on MSA indicates what?

Back 20

Organism does not ferment mannitol; there is no reaction. Therefore, it is a nonpathogenic Staphylococcus.

Front 21

Medium that contains lactose, bile salts, neutral red, and crystal violet

Back 21

MacConkey Agar

Front 22

In MacConkey agar what makes it differential?

Back 22

Bile salts and crystal violet inhibit growth of G+ organisms.

Front 23

What turns MacConkey agar red?

Back 23

Acid accumulation upon lactose fermentation.

Front 24

What is an example of an organism that would ferment MacConkey agar?

Back 24

E. faecalis

Front 25

Enterococcus faecalis is G+/G-.

Back 25

Gram-positive, therefore it can grow on MacConkey agar.

Front 26

T/F E. faecalis is involved in nosocomial infections and has high antibiotic resistance.

Back 26

True

Front 27

E. faecalis is commensal where?

Back 27

GI tract

Front 28

G+; can cause endocarditis and bladder infections.

Back 28

E. faecalis

Front 29

used for isolation and differentiation of the members of Enterobacteriaceae

Back 29

MacConkey agar

Front 30

Three types of Enterobacteriaceae

Back 30

E. Coli, salmonella, proteus

Front 31

Poor growth on MacConkey agar indicates what?

Back 31

Organism is inhibited by crystal violet and/or bile; Gram+

Front 32

Good growth on MacConkey agar indicates what?

Back 32

organism is not inhibited by crystal violet or bile; G-

Front 33

Pink to red growth on MacConkey agar indicates what?

Back 33

Organism produces acid from lactose fermentation; possible coliform

Front 34

Growth is "colorless" (not red or pink) on MacConkey agar. What does this mean?

Back 34

Organism does not ferment lactose. It is noncoliform.

Front 35

contain a protein receptor capable of reacting with fibrinogen

Back 35

s. aureus

Front 36

the protein receptor capable of reacting with fibrinogen

Back 36

coagulase

Front 37

functions as adhesion protein

Back 37

coagulase

Front 38

adhesion protein that allows Staph to coat itself with fibrinogen, preventing phagocytosis

Back 38

coagulase

Front 39

tests for the presence of coagulase

Back 39

staph agglutination

Front 40

coagulase allows Staph to coat itself with what?

Back 40

fibrinogen

Front 41

What is a positive staph agglutination result?

Back 41

clumping appears in the R1 reagent after 30 seconds

Front 42

No clumping in R1 and R2

Back 42

negative staph agg

Front 43

If control show agglutination, what does this mean?

Back 43

the test is unreliable

Front 44

medium that grows more than one type of organism and displays differences in each

Back 44

differential medium

Front 45

T/F MSA is both selective and differential.

Back 45

True

Front 46

What is the selective factor in the MSA medium?

Back 46

NaCl

Front 47

What genus can grow on MSA?

Back 47

Staphylococcus

Front 48

T/F MacConkey agar is useful for isolation and differentiation of the members of enterobacteriaceae.

Back 48

True

Front 49

the adhesive protein which allows Staphylococcus aureus to coat itself with fibrinogen, thereby preventing phagocytosis by phagocytic cells?

Back 49

coagulase

Front 50

What is the Staph agglutination kit testing for?

Back 50

coagulase

Front 51

Which organism will ferment a component in the MSA plate?

Back 51

S. aureus

Front 52

Is Enterococcus faecalis G- or G+?

Back 52

G+

Front 53

T/F E. faecalis can cause bladder infections.

Back 53

True