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45 Cards in this Set

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Exotoxins contained by some G+ cocci
hemolysins
enriched differential media
blood agar
allows differentiation of bacteria based on ability to hemolyze RBCs
Blood agar
Blood agar allows differentiation of bacteria based on what?
ability to hemolyze RBCs
What are the three types of destruction of RBCs and hemoglobin?
alpha, beta, gamma
partial destruction of RBCs; leads to greenish discoloration around colonies
alpha hemolysis
complete destruction of RBCs; leads to clearing around the colonies
Beta hemolysis
no hemolysis; just bacterial growth
gamma hemolysis
hemolysins produced by Streptococcus
streptolysins
streptolysin with maximal activity under anaerobic conditions
type o
streptolysin that expresses itself optimally under aerobic conditions
Type S
streak for isolation and then stab with a loop
streak stab technique
What is the purpose of stabbing the plate with the loop?
The stab encourages the streptolysin activity due to reduced oxygen concentration in the subsurface envi.
streptococcus pneumoniae
G+; alpha hemolytic
streptococcus pyogenes
beta hemolysis; produces streptolysins O and S
What do hemolysins do?
Kill RBCs and hemoglobins
The process in which RBCs and hemoglobins cells are destroyed
hemolysis
What are the three types of hemolysis?
alpha, beta, gamma
What type of Streptococcus causes strep throat?
S. pyogenes
T/F M protein is present in the S. aureus capsule.
True
What is the enzyme which breaks down H2O2?
catalase
If you add H2O2 to a culture and see bubbles what does this tell you?
The organism is catalyzed
Which test can distinguish between G- bacteria (like P. aeruginosa) and enterobacteriaceae?
oxidase test
T/F EMB agar is both selective and differential.
True
Vigorous fermenters on EMB will display what characteristic on the agar?
Dark (purple to black) color and metallic sheen
composed of molecules capable of accepting and donating e-
ETC
energy created by ETC is used to generate what?
ATP
What are two cellular toxins sometimes produced during ETC?
H202 and 02
Strep species are catalase (- or +)?
catalase -
Staph and S. marcescens are catalase (- or +)?
catalase +
used for identification and isolation of G- enteric rods?
EMB Agar
bacteria that ferment lactose or sucrose
fecal coliforms
What is a type of fecal coliform?
E.Coli
used in water quality testing; aids in food digestion
fecal coliforms
inhibit growth of most G+ organisms
eosin and methylene blue
the fermentable substances present in EMB media
sucrose and lactose
What is sheen indicative in growth on EMB agar?
vigorous lactose or sucrose fermentation of fecal coliforms
What color indicates slow or weak fermenters on EMB agar?
mucoid pink colonies
responsible of transfer of e- to O2 thus reducing it to H2O
cytochrome c oxidase
What is the chromogenic reducing agent used in oxidase testing?
tetramethyl-p-phenylenediamine
What does tetramethyl-p-phenylenediamine do in oxidase testing?
detects bacteria that produce cytochrome c oxidase
organisms that contain cytochrome c oxidase will do what on BBL DrySlides?
turn purple
Disregard color changes on BBL DrySlides after hoe much time has passed?
20 s
Is E. coli oxidase positive or oxidase negative?
oxidase negative
Is P. aeruginosa oxidase positive or oxidase negative?
oxidase positive