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45 Cards in this Set
- Front
- Back
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Exotoxins contained by some G+ cocci
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hemolysins
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enriched differential media
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blood agar
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allows differentiation of bacteria based on ability to hemolyze RBCs
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Blood agar
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Blood agar allows differentiation of bacteria based on what?
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ability to hemolyze RBCs
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What are the three types of destruction of RBCs and hemoglobin?
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alpha, beta, gamma
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partial destruction of RBCs; leads to greenish discoloration around colonies
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alpha hemolysis
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complete destruction of RBCs; leads to clearing around the colonies
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Beta hemolysis
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no hemolysis; just bacterial growth
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gamma hemolysis
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hemolysins produced by Streptococcus
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streptolysins
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streptolysin with maximal activity under anaerobic conditions
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type o
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streptolysin that expresses itself optimally under aerobic conditions
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Type S
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streak for isolation and then stab with a loop
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streak stab technique
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What is the purpose of stabbing the plate with the loop?
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The stab encourages the streptolysin activity due to reduced oxygen concentration in the subsurface envi.
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streptococcus pneumoniae
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G+; alpha hemolytic
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streptococcus pyogenes
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beta hemolysis; produces streptolysins O and S
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What do hemolysins do?
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Kill RBCs and hemoglobins
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The process in which RBCs and hemoglobins cells are destroyed
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hemolysis
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What are the three types of hemolysis?
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alpha, beta, gamma
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What type of Streptococcus causes strep throat?
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S. pyogenes
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T/F M protein is present in the S. aureus capsule.
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True
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What is the enzyme which breaks down H2O2?
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catalase
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If you add H2O2 to a culture and see bubbles what does this tell you?
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The organism is catalyzed
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Which test can distinguish between G- bacteria (like P. aeruginosa) and enterobacteriaceae?
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oxidase test
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T/F EMB agar is both selective and differential.
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True
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Vigorous fermenters on EMB will display what characteristic on the agar?
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Dark (purple to black) color and metallic sheen
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composed of molecules capable of accepting and donating e-
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ETC
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energy created by ETC is used to generate what?
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ATP
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What are two cellular toxins sometimes produced during ETC?
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H202 and 02
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Strep species are catalase (- or +)?
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catalase -
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Staph and S. marcescens are catalase (- or +)?
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catalase +
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used for identification and isolation of G- enteric rods?
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EMB Agar
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bacteria that ferment lactose or sucrose
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fecal coliforms
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What is a type of fecal coliform?
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E.Coli
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used in water quality testing; aids in food digestion
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fecal coliforms
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inhibit growth of most G+ organisms
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eosin and methylene blue
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the fermentable substances present in EMB media
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sucrose and lactose
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What is sheen indicative in growth on EMB agar?
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vigorous lactose or sucrose fermentation of fecal coliforms
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What color indicates slow or weak fermenters on EMB agar?
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mucoid pink colonies
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responsible of transfer of e- to O2 thus reducing it to H2O
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cytochrome c oxidase
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What is the chromogenic reducing agent used in oxidase testing?
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tetramethyl-p-phenylenediamine
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What does tetramethyl-p-phenylenediamine do in oxidase testing?
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detects bacteria that produce cytochrome c oxidase
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organisms that contain cytochrome c oxidase will do what on BBL DrySlides?
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turn purple
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Disregard color changes on BBL DrySlides after hoe much time has passed?
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20 s
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Is E. coli oxidase positive or oxidase negative?
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oxidase negative
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Is P. aeruginosa oxidase positive or oxidase negative?
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oxidase positive
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