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44 Cards in this Set
- Front
- Back
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Just after decolorizer is applied acid-fast bacteria will appear ___ and non-acid bacteria will appear ______.
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Fushcia and colorless
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After brilliant green is applied as counter stain, acid-fast bacteria will appear _____ while non-acid bacteria appear _____.
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Fuschia and green
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Cellular morphology and appearance mycobacterium smegmatis
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Fuchsia bacilli
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Staphylococcus aureus after acid-fast stain
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Clusters of green cocci
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Gram-negative
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E. Coli
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Most critical step of gram stain
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Decolorization
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If you forgot counter stain at end of stain procedure, gram neg would appear ______ and gram positive would appear _______
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Colorless and purple
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Cellular morphology and Gram reaction for Staphylococcus aureus
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Clusters of gram-positive cocci
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Why is it important crystal violet be contrasting color to safranin
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Difference in color between the two stains allows you to differentiate between gram negative and gram positive cells by observing the color of organism
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Micrococcus luteus
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Spherical
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Purpose of allowing bacteria smear to dry when placed on slide
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Drying helps remove excess water to ensure optimal heat fix
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Streptococci
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Chains of spherically shaped
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Staphylococci
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Clusters of spherically shaped
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Streptobacilli
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Chains of rod-shaped
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Decolorizing agent in spore stain
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Water
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Endospore stain
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Veg. Cells-red spores-green
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Bacteria best stained using acid-fast
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Mycobacterium
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______ bacteria retain carbol fuchsin after acid-alcohol, ______ are decolorized
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Acid-fast and non-acid fast
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Gram neg-pink
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Gram positive (purple)
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Acid-fast positive (pink) and negative (bluish)
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Endospore positive (pink with green)
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Acid-fast negative (bluish-green)
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Endospore negative (pink)
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Escherichia coli (microscopic morphology)
Bacillus Gram, acid, endo negative |
Escherichia coli (culture media)
White, mucoid, glistening, opaque |
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Pseudomonas (microscopic morphology)
Bacillus Gram, acid, endo., negative |
Pseudomonas (culture media)
Med-sm, opaque, circular, serrated, irredescent Smells like grapes |
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Serratia (culture media)
Red, medium, irregular, circular, umbinate |
Serratia (microscopic morphology)
Gram negative, acid negative, endo negative Bacillus |
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Micrococcus (microscopic morphology)
Gram positive Coccus |
Micrococcus (culture media)
Bright/buttery yellow, smaller, round, opaque, entire |
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Staphlococcus aureus (microscopic morphology)
Gram positive Staphylococci arrangement |
Staphylococcus aureus (culture media)
Beige, dry, irredescent, opaque |
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Fomite
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Object or substance capable of carrying infectious organisms
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Aseptically
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Performed under sterile conditions
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Density
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Opaque, transparent, or translucent
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Consistency
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Butyrous, mucoid, dull, dry, or powdery
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Texture
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Smooth, rough, wrinkled, glistening, or irredescent
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Serial dilution
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Stepwise dilution of substance in solution
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Colony forming unit- CFU
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Viable bacteria; colony
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TFTC
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Too few to count
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TNTC
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Too numerous to count
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Nosocomial infection
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Infection development favored by a hospital environment
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Gram stain reagents, time, and purpose
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Crystal violet (primary stain)- 60 sec; Gram's iodine (mordant)-60 sec.; ethyl alcohol (decolorizer) 10-15 sec; safranin (counter stain) 30 sec
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Acid-fast reagents, purpose, and time
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Carbol fuchsin (penetrate cw) 5 min; acid-alcohol (decolorizer) 30 sec; brilliant green (counter stain) 2 min
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Endospore stain reagents and time
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Malachite green-steam 5 min; safranin- 1 min
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Bacillus (microscopic morphology)
Streptobacillus Gram positive, acid neg, endo positive |
Bacillus (culture media)
Beige, dry, rough, transparent |
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Mycobacterium (microscopic morphology)
Bacillus Gram positive, acid positive, and endo negative |
Mycobacterium (culture medium)
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