Micro Lab Quiz

Front 1

EMB

Back 1

Eosin Methylene Blue
Coliforms-
if coliform (lactose): dark colony/ if not: straw
E-coli will have green/black irradescent sheen

Front 2

BPA

Back 2

Staphylococcus Aureus
(rotten eggs over easy)
Black shiny colonies (telluride reduction) with halo of hydrolyzed lecithin

Front 3

VRB

Back 3

Violet Red Bile agar
Coliforms-
if coliform (lactose): Violet Red colony/ if not: straw
E-coli will have halo

Front 4

MSA

Back 4

Mannitol Salt Agar
Staphylococcus Aureus
Red--->yellow since S. aureus ferments mannitol. Cream colored colonies

Front 5

MOX

Back 5

Listeria
Appear as dark black colonies
Black precipitate due to esculin and ferric ammonium citrate

Front 6

PALCAM

Back 6

Listeria
Appear as dark black colonies
Black precipitate due to esculin and ferric ammonium citrate

-MOST- do not ferment mannitol so should not change to yellow if listeria m

Front 7

CAMP

Back 7

Streak plate:
S aureus/ R equi

If L. monocytogenes, enhanced hemolysis @ S aureus

Front 8

XLD

Back 8

Xylose Lysine Desoxycholate
Salmonella
Red colonies with black center
Media is red/ should not change

Front 9

Hektoen Enteric Agar

Back 9

Salmonella
Greenish blue colonies with black dot in center
Media should be greenish/ should not change to all yellow

Front 10

Bismuth Sulfur Agar

Back 10

Salmonella
Black/green colonies may have sheen/ green-gray media should not change color