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21 Cards in this Set

  • Front
  • Back
3 ways to ID bacteria
-biochemical (metabolic) testing
-immunological (antibodies) testing
-genetic (PCR) testing
media where some bacteria grow and some don't
selective media
media that favors/encourages certain bacteria
enrichment media
media that gives you additional information about organisms that grow on it
differential media
in mannitol salt, the high NaCl selects for __
gram + bacteria
in mannitol salt, the high NaCl selects against __
gram - bacteria
staphylococcus aureus ferments mannitol and turns the solution __ with pH indicator phenol red
yellow
EMB selects for __
gram -
EMB inhibits __
gram +
the differential in EMB (eosin methonal blue) is __
lactose
bacteria uses __ to break down lactose
beta-galactosidase
when bacteria ferment EMB it turns
green
ie lactose and sucrose
disaccharides
ie startch
polysaccharides
bacteria excrete __ (an exoenzyme) to digest startch
amylase
in a statch augar plate, a clear ring around smear indicates __
the abscense of startch
the inverted tube that traps gas in a liquid culutre
durham tube
what is the concentration of sugars in a tripple sugar iron augar medium?
glucose 1x
sucrose 10x
lactose 10x
the iron reagent in triple sugar augar iron agar detects __
H2S
what does a yellow butt indicate in a TSIA medium?
glucose only is fermented
what does it mean if the slant and butt are yellow in TSIA?
glucose and sucrose and or lactose fermented