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37 Cards in this Set
- Front
- Back
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UV light how to prepare the test and expose the bacteria to UV light.
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1. Make a pour 2. streak w/bacteria 3. take plate to UV light and remove lid to plate agar side up. 4. place an index card over I/2 of the plate for the assigned time. 5. replace lid and incubate @ 30⁰C |
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What effect does the UV light has on the bacteria?
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UV radiation causes the formation of Thymine dimers in DNA. G T T G C A A C |
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UV light results
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G T T G C A A C These end up creating mutations in the org genetic code causing cell reproduction & protein syn problem that will eventually inhibit growth. |
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Nitrate reduction test, test for what substance?
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Nitrate
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Nitrate reduction test, test for what?
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To see if an anaerobe can use nitrate as a final e- acceptor.
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Tryptophan hydrolysis (indole) test, test for what?
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Test to see if bacteria can break down amino acid tryptophan to make pyruvic acid that can be shuttled into cellular respiration
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Tryptophan hydrolysis (indole) test, test for what substance?
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The presence of indole
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What color is a negative results in the indole test?
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Neon green ring
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MR test what color is a negative results?
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Clear
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Alcohol fermentation: At what temperature does yeast best fermentate sugar to produce alcohol?
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37⁰C
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Explain how to do the alcohol fermentation test?
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1. add 15ml of grape juice in a flask 2. add 3ml activated yeast 3. put a deflated balloon over flask opening and seal it. 4. then put in incubator |
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What are we looking for in the Synder Test?
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A bacteria that causes cavities
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How to prepare for a Synder Test?
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1. Put a tube of agar in water and allow it to boil H₂O and melt agar 2. once the agar turn into a liquid take it out and wait until warm to touch. 3. spit into tube (after sucking your teeth) 4. incubate @ 37⁰C |
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What is an example of the bacteria in the Synder Test?
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Streptococcus Mutans
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What type of agar is used in the Synder Test?
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2% glucose and the pH indicator bromcresol green
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What color does the agar turn if the test is positive? |
Color ∆ from green to yellow if the bacteria is presence
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Blood Agar differentiate what?
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differentiate B/T bacteria ability to hemolyze RBC's
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Blood agar symbol γ mean
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γ mean No hemolysis just colonies
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Blood agar symbol ∝ mean
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∝ mean hemolysis → incomplete hemolysis → metallic green ting around colonies (maybe clear arear) |
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Blood agar symbol β mean
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β mean hemolysis → complete hemolysis→ clear zones around colonies |
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Give an example of bacteria found in Blood Agar?
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Streptococcus Pyogenes
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Antibiotic resistance explain how to prepare the test.
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1. make a pour 2. place 20 loopful of bacteria in center of plate & spread. 3. divide in to 4 quadrant 4. place 4 different antibiotic disc on the plate 5. incubate at 30⁰C with the plate right side up |
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antibiotic resistance test results
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1. resistance → able to grow in presence of substance. 2. Suceptible → ∅ able to grow in presence of substance 3. inhibition → areas surrounding antibiotic disc where growth occurred 4. Bigger zone, less resistance, more susceptible |
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VP → vortex every ______ mins for ______ mins.
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5 mins, 30 mins
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Catalase test to see if bacteria use ________ as an enzyme to neutralize _____________.
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Catalase, toxic forms of oxygen
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How to prepare for oxidate test.
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Pick up bacteria on a swab then apply oxidate reagent |
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Oxidate uses __________ as a _________ in the ________. |
Proteins/enzymes, e- carrier, ETC
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Oxidate results: + Ox = - Ox = |
+ Ox =blus - Ox = ∅ blue |
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Urea results: + urease = - urease = |
+ urease =Pink - urease = salmon |
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Urea is broken down by __________ to __________.
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urease, NH₃ + CO₂
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Citrate convert _________________ into _______________. |
Ammonium salts, ammonia
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Citrate the ____________ raise the _______.
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NH₃, PH
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Citrate color results: + Citrate = - Citrate = |
+Citrate = blue - Citrate = green |
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TSI break down __________ by an enzyme _____________ to ________, _________, _________. |
Cysteine →Cysteine → Pyruvic + NH₃ + H₂S Desulfrase acid |
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TSI there is _______ in the media. The ____________ will bind to ___________. |
iron, Hydrogen Sulfide gas, iron & rust
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TSI is much like __________________ only ________ causes _________ to turn in a _________________, precipitate. |
O₂ rusts/iron, H₂S, iron black iron sulfide
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TSI results: +H₂S = - H₂S = |
+H₂S = Black fuzzy caterpillar - H₂S = ∅ Black fuzzy caterpillar |
