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61 Cards in this Set
- Front
- Back
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Stapphylococcus Aureus color |
Yellow |
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Micrococcus Luteus color |
Yellow |
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Micrococcus Roseus color |
Pale pink |
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Pseudomonas Aeruginosa color |
Green |
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Serratia Marcessens color |
Red |
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Are Rods motile or non-motile? |
Motile |
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Are Gram (-) motile or non-motile? |
Motile besides K.A. |
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Are Cocci motile or non-motile? |
Non-motile |
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Count |
25-250 cfu |
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Scanning Power |
4x |
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Low Power |
10x |
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High Power |
40x |
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Oil Immersion |
100x |
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Contrast of Positive Stain |
Stain the cells (light background) |
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Negative Stain |
Stain the background (light cells) |
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Resolution on Microscope |
2 images close together, make clear |
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Parcentral on Microscope |
Once field of view is set, move mechanical stage does not change field of view |
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Parfocal on Microscope |
Not adjusting coarse focus, just fine focus |
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Total Magnification = |
Obj x Ocular |
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Slant to Broth |
Loop |
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Slant to Slant |
Loop and needle |
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Slant to Deep |
Needle |
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Mixed Broth to plate |
Loop |
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Gram Stain Primary Stain |
Crystal Violet |
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Gram Stain Mordant |
Gram's Iodine |
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Gram Stain De-colorizing Agent |
95% Ethyl Alcohol |
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Gram Stain Counterstain |
Safranin |
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Gram (+) Bacteria (7) |
1. Stapphylococcus 2. Streptococcus 3. Micrococcus 4. Bacillus 5. Clostridium 6. Mycobacterium 7. Corynebacterium |
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Gram (-) Bacteria (7) |
1. Alcaligenes 2. Pseudomonas 3. Escherica 4. Citrobacter 5. Enterobacter 6. Serratia 7. Proteus |
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Acid Fast Stain Primary Stain |
Carbofuchism |
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Acid Fast Stain Decolorizing Agent |
Acid Alcohol |
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Acid Fast Counterstain |
Methylene Blue |
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Endospore Stain used on |
Bacillus and Clostridium |
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Endospore Primary Stain |
Malachite Green |
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Endospore Counterstain |
Safranin |
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Color of Endospore Stain |
White with green halo |
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Capsule Stain used on |
Mycobacterium, Streptococcus and Klebsiella Pneumonae |
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Capsule Stain Primary Stain |
Methylene Blue |
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Capsule Stain Background Stain |
Nigrosin |
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Mannitol Salt Agar Halo Tolerant |
Staphylococcus, Micrococcus |
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Mannitol Salt Agar Nonhalotolerant |
E. Coli |
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Mannitol Salt Agar Ferments |
Mannitol Indicator - Phenol Red |
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Macconkey's Agar Stains E.Coli |
Bright Pink |
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Macconkey's Agar Ferments |
Lactose |
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Eosin Methylene Blue Agar stains E.Coli |
Green |
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Eosin Methylene Blue Agar Ferments |
Lactose |
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Types of Media |
1. Synthetic/Defined Media 2. Complex Media 3. Enriched Media 4. Environmental Media 5. Anaerobic Media |
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Anaerobic Media Thioglycolate Broth indicator |
Resazum (red for oxygen) |
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Anaerobic Media Anaerobe Jar indicator |
Methylene Blue (oxygen blue) (no oxygen white) |
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Anaerobic Media Anaerobe Jar catalyst |
Palladium |
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Obligate Aerobe |
Grows at the top |
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Faculative Anaerobe |
Grows at the top, will grow elsewhere |
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Obligate Anaerobe |
Grows at the bottom |
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Microaerophillic |
Grows in the middle |
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Aerotolerant |
Grows throughout |
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Zone of Inhibition |
Diameter of no growth |
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Best Disinfectants |
Hydrogen Peroxide 3%, Clorox, Iodine |
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Arrangement of Bacillus |
Chains |
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Arrangement of Streptococcus |
Diploid |
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Arrangement of Micrococcus |
Tetrad |
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Arrangement of Staphylococcus |
Clusters |
