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16 Cards in this Set
- Front
- Back
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Mannitol Salt Agar (MSA)
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Selective and differential
Selects for micrococcus and staphylococcus via osmotic pressure. Yellow - mannitol fermenter (s. aureus) |
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Eosin Methylene Blue (EMB)
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Selective and differential
Selects for gram negative. Dark green/purple - strong lactose/sucrose fermenter Pink - weak fermenter No color - no fermentation. still gram - |
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MacConkey Agar (MAC)
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Selective and differential
Selects for gram - Pink - lactose fermenter Colorless or tan - non lactose fermenter |
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Phenylethyl Alcohol Agar (PEA)
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Selective
Selects for gram positive cocci. Limited growth in gram - |
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Colistin Nalidixic Acid Agar (CNA)
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Selective
Isolates gram positive bacteria. |
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Phenol Red Broth (PR)
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Lactose, sucrose, glucose (dex)
Differential, non-selective. Red - non fermenter Pink/orange - degradation of peptone Yellow w/o bubble - fermentation Yellow w/ bubble - fermentation with gas production |
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MRVP Broth
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Methyl Red - add 3 drops. Immediate red appearance indicates strong acid production during fermentation. Yellow - lac of strong acid production.
VP - add 15 drops of Barritt's Reagent A (naphthol) and then 5 drops of Barrit's Reagent B (potassium hydroxide). Wait 60 min. Red - acetoin production. Yellow - no acetoin production. |
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Catalase Enzyme
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Differential, non-selective
On slide. Add 3% hydrogen peroxide. Bubbles - catalase activity No bubbles - no catalase activity |
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BBL Oxidase DrySlide
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4 quarter slide. Determines presence of cytochrome c oxidase. Purple means cytochrome is there.
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Simmons Citrate Agar
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Differential, non-selective
Blue - organism uses citrate Green - organism unable to use citrate |
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Phenylalanine Agar Slant
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Differential, non-selective
Reagent - 10% Ferric Chloride (5-10 drops) Turns green- Phenylalanine deaminase production. Remains yellow - no phenylalanine deaminase produced. |
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Starch Agar
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Differential, non-selective
Flood plate with gram's iodine Purple near growth - no starch hydrolysis Clear near growth - starch hydrolysis/amylase production |
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Urea Broth
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Differential, non-selective
Pink color in medium - urease production no change - no urease production |
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SIM Agar
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Differential, non-selective
Reagent - Kovacs Reagent (10 drops) Growth away from line - motility Growth restricted - non motile Black precipitate - hydrogen sulfide production Kovac's pink - indole production Kovac's yellow - no indole production. |
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Kliger Iron Agar
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differential
Red butt / slant - no fermentation Yellow butt / slant - glucose/lactose/sucrose fermentation Yellow butt / red slant - glucose fermentation Bubble or break in agar - gas production Black precipitate - hydrogen sulfide production |
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Blood Agar
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Differential
Alpha hemolysis - green halo Beta hemolysis - clear zone Gamma hemolysis - no lytic zone |
