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11 Cards in this Set
- Front
- Back
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Mannitol Salt Agar (M.S.A.)
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Nutrient-Mannitol
Inhibitor-7.5% NaCl Indicator-phenol red (acid-yellow, base-pink) |
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Mannitol Salt Agar Organisms
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Staphlococci-normal
S. aureus-yellow |
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Phenlyethyl Alchol Agar (P.E.A.)
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Selective for G +, inhibits the growth of G(-), by interfering with DNA synthesis.
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Eosin Methlyene Blue Agar
(E.M.B.) |
Nutrients-peptone, lactose, sucrose
Inhibitor-Dyes Eosin y, Methylene blue Idication-(purple-acid from fecal coliforms)(pink-slow fermenters) |
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EMB Organisms
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Gram-negative Rods
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Hektoen Enteric Agar (H.E.A.)
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Gram-Positive selective
Pink-acid production from lactose H2S forms from Iron formation when positive |
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MacConkey Agar (M.A.)
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Selective-Bile Salts(for G(-))Crystal Violet(G(-))
Nutrient-lactose Diff-Neutral Red(bright red when acidic) |
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Oxidation-Fermentation (O-F) Test
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Will tell if organisms are Oxidative(unsealed yellow), Fermentative(Sealed Yellow), or both
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Phenol Red Broth
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Will have different sugars, and glass "gas tubes" to test for gas production. Yellow-acid(with or without gas), Red-no fermentation, Pink(Degradation-peptone)
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Methly Red & Voges-Prokauser Tests(MR-VP)
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Organism grown in broth, Methylred added to one, reagent A&B added to the VP.
MR-red=mixed acid ferment VP-red=2,3-butanediol ferment(acetoin produced) |
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Catalase Test
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Tests for Catalyse, If bubble catalyse is present and it respires
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