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15 Cards in this Set

  • Front
  • Back
Purpose and Media for Fluid Thioglycolate?
Purpose: The use of this both permits growth of anerobic bacteria
Media: Glucose, Cysteine, sodium thiogllycolate
Fluid Thioglycolate indicator
Resazurin (shows on top and indicates oxygen)
Mannitol Salt Agar purpose and media?
Purpose: mannitol provides substrate for fermentation
Media: Mannitol and Sodium Chloride
Mannitol indicator and positive result?
Indicator: Phenol Red
+ Bright yellow streak
- no color change: pink
EMB purpose and media?
Purpose: which ferment lactose (coliforms)?
Media: Eosin, MB, lactose, sucrose
EMB indicator and + result?
Indicator: Eosin Y and MB
+ Metalic green / purple
- Colorless
MacConkey purpose and media?
Purpose: Which one lactose ferment?
Media: Bile salts
MacConkey indicator and + result?
Indicator: Crystal Violet, neutral red dye
+ hot pink
- white, colorless
Durham tube purpose and media?
Purpose: using 3 diff sugars, which ones ferment acid?
Media: dextrose, lactose, sucrose, peptone,
Durham tube indicator and + result?
Indicator: Phenol red
+ yellow means acid
- no color change, red
bubble in tube: gas
Indole test purpose and media used?
Purpose: production of enzyme tryptophanase?
Media: Tryptone agar plate
Indole indicator and + result?
Indicator: indole dry slide
+ color change to red in 30 sec
- no color change
Methyl red purpose and media?
Purpose: bacteria using mixed acid fermentation pathway?
Media: buffered peptone glucose broth
Methyl red indicator and + result?
Indicator: methyl red
+ immediate bright red on top AFTER adding methyl red
- yellow, orange
Voges purpose and medai?
Purpose: