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50 Cards in this Set
- Front
- Back
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Media name & Contents: Urea Hydrolysis
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Urea broth with phenol red
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Media name & Contents: Citrate Utilization
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Simmon's citrate slant with pH indicator
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Media name & Contents: Hydrogen sulfide production
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Sim Deep with cysteine and Fe compound
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Media name & Contents: Nitrate Reduction
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Nitrate broth
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Media name & Contents: Indole test
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Sim Deep with tryptophan
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Media name & Contents: Catalase test
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TSA or any media for growth
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Media name & Contents: Oxidase test
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TSA or any media for growth
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Media name & Contents: Carbohydrate fermentation (Glucose/Lactose)
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Glucose broth w/phenol red or Lactose broth w/inverted Durham tube
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Media name & Contents: MR test
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MRVP broth containing glucose
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Media name & Contents: VP test
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MRVP broth containing glucose
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Theory: Urea Hydrolysis
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If the organism produces enzyme Urease, it will be broke down into ammonia and CO2
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Theory: Citrate Utilization
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If the organism uses citrate as sole carbon source, organism will grow & produce alkaline, increasing the pH
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Theory: Hydrogen sulfide production
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If the organism can break down cystein, H2S will be released. The H2S will react with Fe
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Theory: Nitrate Reduction
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NO3--> NO2--> N2 (gas)
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Theory: Indole test
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If organism can break down tryptophan, indole will be produced
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Theory: Catalase test
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If organism produces catalase, enzyme will convert H2O2 to H2O+O2 (gas)
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Theory: Oxidase test
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Oxidase is final step of cell respiration, enzyme catalase reaction with O2 as terminal electron acceptor.
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Theory: Carbohydrate fermentation (Glucose/Lactose)
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If fermented the sugar will produce an acid, lowering pH
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Theory: MR test
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Methyl red will turn red due to the low pH, if large amounts of acid have been produced
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Theory: VP test
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Reagent will turn red if an alcohol precurser molecule is present
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Chemical reaction and Enzyme (if known): Urea Hydrolysis
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Urease broke down into ammonia & CO2
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Chemical reaction and Enzyme (if known): Citrate Utilization
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No Enzyme
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Chemical reaction and Enzyme (if known): Hydrogen sulfide production
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Cysteine ----> Cysteine desulfurase ----> H2S + Carbon compound (food)
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Chemical reaction and Enzyme (if known): Nitrate Reduction
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No Enzyme
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Chemical reaction and Enzyme (if known): Indole test
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Tryptophan ---> Tryptophanase ---> Indole + Carbon compound (food)
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Chemical reaction and Enzyme (if known): Catalase test
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H2O2 ---> Catalase ---> H2O + O2 (gas)
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Chemical reaction and Enzyme (if known): Oxidase test
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Oxidase
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Chemical reaction and Enzyme (if known): Carbohydrate fermentation
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No Enzyme
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Chemical reaction and Enzyme (if known): MR test
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No Enzyme
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Chemical reaction and Enzyme (if known): VP test
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No Enzyme
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Reagents added after incubation: Urea Hydrolysis
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None
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Reagents added after incubation: Citrate Utilization
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None
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Reagents added after incubation: Hydrogen sulfide production
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None
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Reagents added after incubation: Nitrate Reduction
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Nitrate A reagent and Nitrate B reagent
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Reagents added after incubation: Indole test
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Kovac's
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Reagents added after incubation: Catalase test
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H2O2
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Reagents added after incubation: Oxidase test
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Oxidate reagent
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Reagents added after incubation: Carbohydrate fermentation
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None
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Reagents added after incubation: MR test
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Methyl red
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Reagents added after incubation: VP test
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VP I reagent and VP II reagent
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Test appearance (+)/(-): Urea Hydrolysis
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(+) Hot Pink (-) Peach/Orange
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Test appearance (+)/(-): Citrate Utilization
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(+) Blue & growth (-) Green & no growth
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Test appearance (+)/(-): Hydrogen sulfide production
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(+) Black (-) No color
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Test appearance (+)/(-): Nitrate Reduction
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A&B (+) Red (-) No color ---- Zn dust (+) No Color (-) Red
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Test appearance (+)/(-): Indole test
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(+) Red (-) No Red
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Test appearance (+)/(-): Catalase test
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(+) Bubbles (-) No bubbles
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Test appearance (+)/(-): Oxidase test
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(+) Purple (-) No color within a minute
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Test appearance (+)/(-): Carbonhydrate fermentation
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(+) Cloudy w/yelo broth w/ or w/out bubble (-) Cloudy w/red & no bubble
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Test appearance (+)/(-): MR test
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(+) Red (-) No Yelo
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Test appearance (+)/(-): VP test
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(+) Red (-) Brown/green
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