Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
48 Cards in this Set
- Front
- Back
|
Independent Variable of:
Leaky roofs in peanut butter factories increases the changes of Salmonella getting into the product. |
leaky roof
|
|
|
Dependent Variable of:
Leaky roofs in peanut butter factories increases the changes of Salmonella getting into the product. |
salmonella in pb?
|
|
|
Independent Variable of:
Only 1% of the adult population will have GI tract side effects to this new antibiotic. |
antibiotics and your genetics
|
|
|
Dependent Variable of:
Only 1% of the adult population will have GI tract side effects to this new antibiotic. |
% of people with side effects
|
|
|
Independent Variable of:
Fast food restaurants are contributing to an increase in adult onset diabetes. |
food
|
|
|
Dependent Variable of:
Only 1% of the adult population will have GI tract side effects to this new antibiotic. |
whether people get diabetes
|
|
|
Independent Variable of:
Using non-sterile contact lens cleaner will increase your risk of getting ulcerative keratitis. |
non-sterile contact lens cleaner
|
|
|
Dependent Variable of:
Using non-sterile contact lens cleaner will increase your risk of getting ulcerative keratitis. |
whether or not get keratitis
|
|
|
Gram positive organisms accept the counter stain.
|
False
|
|
|
The function of iodine in the gram stain is to...
|
hold in the primary stain
|
|
|
A direct gram stain of a throat swab is essential to final identification of any pathogenic Strep.
|
False
|
|
|
Our initial isolation of enterics in our kitchens was based on which metabolic test?
|
lactose usage
|
|
|
All bacteria are either gram positive or gram negative.
|
False
|
|
|
The procedures for routine cultures includes:
|
incubation at 35'C aerobically
|
|
|
The most prominent normal flora in the throat are...
|
alpha and gamma strep
|
|
|
Mannitol salt media is used to identify...
|
Staph aureus
|
|
|
A positive slide for gonorrhea would show...
|
gram negative diplococci in WBC's
|
|
|
What do the test for lysine, the test for indole and the test for hydrogen sulfide have in common?
|
they are all testing for some amino acid use
|
|
|
What is the purpose of the streak plates?
|
to isolate colonies
|
|
|
Endospore stain
|
see green spores and red cell walls of the mother cells....must be heated for at least 10 minutes before the spores will accept the dye
|
|
|
MIC
|
minimal inhibitory concentration
|
|
|
When may intermediate drugs be a good choice?
|
-Bladder infections
-Allergies -if org is resistant to numerous antibiotics |
|
|
Turbidity Standard
|
McFarland 0.5 turbidity represents a cell count of 1.5 X 10^8 cells per ml
|
|
|
Differential Media
|
allows you to differentiate between various organisms
|
|
|
Differential Media Example:
|
TSA with 5% sheep blood
|
|
|
Beta hemolysis
|
clear zones; RBC's all ruptured or hemolysed
|
|
|
Alpha hemolysis
|
green zones' RBC's partially ruptured or partial hemolysis
|
|
|
Gamma hemolysis
|
no zones or change in media around the colonies. no hemolysis occurs
|
|
|
Selective Media
|
allows some organisms to grow and suppresses others
|
|
|
Selective Media Example:
|
mannitol salt plates
|
|
|
When does phenol red turn yellow?
|
under acidic conditions
|
|
|
SS agar
non-pathogens have... |
lactose positive colonies that are bright pink
|
|
|
SS agar
pathogens show... |
lactose negative colonies that are colorless
|
|
|
EMB agar
non-pathogens are... |
lactose positive with pink colonies and purple centers
|
|
|
EMB agar
E-coli |
has a very distinctive growth pattern of metallic green colonies
|
|
|
EMB agar
pathogens are... |
lactose negative and have colorless, clear colonies
|
|
|
SIM
|
can be used to detect hydrogen sulfide production, indole production and motility of your unknown
|
|
|
H2S
|
if an organism can use AA's containing sulfur, they will often create H2S to bind to the iron. a black ppt. will form thus indicating hydrogen sulfide is present
|
|
|
Indole production
|
indole is a waste product produced when organisms use the AA tryptophan
|
|
|
What does it mean if the indole turns red?
|
positive result
|
|
|
Detecting lactose and glucose
|
pH indicator turns yellow if org is positive
if org doesn't use sugars BUT can use the proteins, color turns red-purple |
|
|
If bacteria are positive for urease...
|
will yield a pink tube
|
|
|
If bacteria are negative for urease....
|
yellow tube
|
|
|
Lysine test
|
turns yellow when the pH is acidic (negative) and purple when pH is alkaline (positive)
|
|
|
Factors that affect germicidal activity....
|
-concentration
-length time the org is exposed -porous vs. non-porous surface |
|
|
o and p
|
ova and parasites
|
|
|
hemoflagellates
|
lives in the blood stream; extracellular parasites
|
|
|
Plasmodium vivax
|
intracellular parasites
|
