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48 Cards in this Set

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Independent Variable of:
Leaky roofs in peanut butter factories increases the changes of Salmonella getting into the product.
leaky roof
Dependent Variable of:
Leaky roofs in peanut butter factories increases the changes of Salmonella getting into the product.
salmonella in pb?
Independent Variable of:
Only 1% of the adult population will have GI tract side effects to this new antibiotic.
antibiotics and your genetics
Dependent Variable of:
Only 1% of the adult population will have GI tract side effects to this new antibiotic.
% of people with side effects
Independent Variable of:
Fast food restaurants are contributing to an increase in adult onset diabetes.
food
Dependent Variable of:
Only 1% of the adult population will have GI tract side effects to this new antibiotic.
whether people get diabetes
Independent Variable of:
Using non-sterile contact lens cleaner will increase your risk of getting ulcerative keratitis.
non-sterile contact lens cleaner
Dependent Variable of:
Using non-sterile contact lens cleaner will increase your risk of getting ulcerative keratitis.
whether or not get keratitis
Gram positive organisms accept the counter stain.
False
The function of iodine in the gram stain is to...
hold in the primary stain
A direct gram stain of a throat swab is essential to final identification of any pathogenic Strep.
False
Our initial isolation of enterics in our kitchens was based on which metabolic test?
lactose usage
All bacteria are either gram positive or gram negative.
False
The procedures for routine cultures includes:
incubation at 35'C aerobically
The most prominent normal flora in the throat are...
alpha and gamma strep
Mannitol salt media is used to identify...
Staph aureus
A positive slide for gonorrhea would show...
gram negative diplococci in WBC's
What do the test for lysine, the test for indole and the test for hydrogen sulfide have in common?
they are all testing for some amino acid use
What is the purpose of the streak plates?
to isolate colonies
Endospore stain
see green spores and red cell walls of the mother cells....must be heated for at least 10 minutes before the spores will accept the dye
MIC
minimal inhibitory concentration
When may intermediate drugs be a good choice?
-Bladder infections
-Allergies
-if org is resistant to numerous antibiotics
Turbidity Standard
McFarland 0.5 turbidity represents a cell count of 1.5 X 10^8 cells per ml
Differential Media
allows you to differentiate between various organisms
Differential Media Example:
TSA with 5% sheep blood
Beta hemolysis
clear zones; RBC's all ruptured or hemolysed
Alpha hemolysis
green zones' RBC's partially ruptured or partial hemolysis
Gamma hemolysis
no zones or change in media around the colonies. no hemolysis occurs
Selective Media
allows some organisms to grow and suppresses others
Selective Media Example:
mannitol salt plates
When does phenol red turn yellow?
under acidic conditions
SS agar
non-pathogens have...
lactose positive colonies that are bright pink
SS agar
pathogens show...
lactose negative colonies that are colorless
EMB agar
non-pathogens are...
lactose positive with pink colonies and purple centers
EMB agar
E-coli
has a very distinctive growth pattern of metallic green colonies
EMB agar
pathogens are...
lactose negative and have colorless, clear colonies
SIM
can be used to detect hydrogen sulfide production, indole production and motility of your unknown
H2S
if an organism can use AA's containing sulfur, they will often create H2S to bind to the iron. a black ppt. will form thus indicating hydrogen sulfide is present
Indole production
indole is a waste product produced when organisms use the AA tryptophan
What does it mean if the indole turns red?
positive result
Detecting lactose and glucose
pH indicator turns yellow if org is positive
if org doesn't use sugars BUT can use the proteins, color turns red-purple
If bacteria are positive for urease...
will yield a pink tube
If bacteria are negative for urease....
yellow tube
Lysine test
turns yellow when the pH is acidic (negative) and purple when pH is alkaline (positive)
Factors that affect germicidal activity....
-concentration
-length time the org is exposed
-porous vs. non-porous surface
o and p
ova and parasites
hemoflagellates
lives in the blood stream; extracellular parasites
Plasmodium vivax
intracellular parasites