Micro Lab

Front 1

Definition of an Enteric

Back 1

Facultative Anaerobic Gram(-) rod that ferments glucose into acid...Has a sub category called coliforms

Front 2

Definition of a Coliform

Back 2

Facultative Anaerobic gram(-) rod that ferments lactose to acid... Also considered an enteric.

Front 3

Catalase test, what is positive and what is negative?

Back 3

+ for Staph

- for strep

Front 4

Facultative Anaerobic definition

Back 4

organism is able to create ATP by aerobic respiration of oxygen is present, but is also able to ferment sugars when there is absence of oxygen.

Front 5

SIM - Media Test.

1. What three things is the media testing for?

2. What are the positive and negatives when adding Kovach's solution?

Back 5

Deep semi solid agar.

1. Reduction of sulfur, motility, and presence of tryptophanase.

2. Cherry red = (+)

Yellow = (-)

Front 6

Phenylalanine Deaminase Test.

1. What does this media look like?

2. What is added, what does it test for, and what are the positives and negatives?

Back 6

1.A slant agar with high concentration of phenylalanine.

2. Once ferric chloride is added, the presence of phenylalanine deanimase is determined.

Green=(+)

Yellow=(-)

Front 7

Lysine Decarboxylation Test.

1. What does media look like?

2. What does media contain?

3. What happens when there is Low pH in this media?

4. What occurs because of the low pH?

5. Give the positives and negatives.

Back 7

1. Normal agar with layer of mineral oil.

2. Contains peptone, glucose, pH indicator, and coenzyme pyridoxal phosphate.

3. Low pH turns the media yellow.

4. Causes decarboxylation positive organisms to produce the enzyme. When this happens lysine is broken down.

5. Purple = (+)

Orange = No result

Yellow = (-)

Front 8

Urea Hydrolysis Test

1. What does media look like?

2. What does it test for?

3. What does it contain?

4. Give positives and negatives.

Back 8

1. Salmon/ pale orange colored normal agar.

2. Tests for an enterics ability to metabolize urea.

3. Urea and phenol red.

4. red/bright pink = (+)

salmon/orange = No result

Yellow = (-)

Front 9

Citrate Test

1. What does media look like?

2. What does it test for?

3. Able to differentiate what organism from other enterics?

4. Give positives and negatives, along with why.

Back 9

1. Green colored slant.

2. Tests enterics ability to use citrate and sole carbon source.

3. Enterobacteriae from other enterics.

4. Blue = (+) citrate is metabolized and creates a base

Green = (-) no citrate was digested, and no growth should be seen.

Front 10

Methyl Red Test

1. What does media look like?

2. What does it contain?

3. What does it test for?

4. Give positives and negatives.

Back 10

1. Straw colored liquid media.

2. peptone, glucose, and phosphate buffer.

3. tests for ability of organism to perform mixed fermentation.

4. Once methyl red is added... pastel red =(+)

pastel orange/yellow= (-)

Front 11

Phenol Red test

1. What does media look like? What is also something physically placed in the media?

2. What does media contain?

3. Tests for what?

4. Gives positives and negatives.

Back 11

1. red media, with a durham tube to catch gas.

2. peptone and pH indicator phenol red.

3. tests for acid production due to fermentation.

4. yellow = (+) because acid is present

red/pink = (-) because deamination occured causing pH to rise.

Front 12

Oxidase test

1. Designed to test for what?

2. Give positives and negatives.

3. If positive, most likely what is determined?

Back 12

1. Designed to test for presence of cytochrome c oxidase.

2. Blue = (+)

No color = (-)

3. if positive, most likely not an enteric, but it is possible to still be an enteric.

Front 13

O/F Test

1. What does media look like?

2. Designed to do what?

3. What will an enteric do? A non-enteric?

4. Give positives and negatives?

Back 13

1. 2 test tubes of green semi-solid media with one containing a oil layer.

2. Designed to test for oxidative metabolism and fermentation capabilities.

3. enteric will make both test tubes yellow. Non-enteric will make unsealed container yellow, or possibly blue.

4. Yellow = (+)

Green/Blue = (-)

Front 14

MacConkey

1. what does media contain?

2. what does it determine?

3. Selective? Differential? Both?

Back 14

1. lactose, bile salts, neutral red pH idicator, and crystal violet.

2. Bile salts and crystal violet inhibit gram(+) growth, neutral red turns red below pH 6.8 which shows lactose fermentation.

3. Both

Front 15

EMB

1. what does media contain?

2. what does it determine?

3. Selective? Differential? Both?

Back 15

1. Peptone, lactose, sucrose

2. differentiates enterics and inhibits gram(+) growth.

3. both

Front 16

H-E Agar

1. What does the media contain?

2. What does it test for?

3. What do the bile salts do?

4. In all, what could the media determine?

5. Selective? Differential? Both?

Back 16

1. Lactose, sucrose, salicilin, bile salts, and ferrous iron.

2. tests for microbes ability to reduce sulfur to hydrogen sulfide, and lactose fermentation.

3. Inhibit Gram(+) growth.

4. possible enterics will grow, lactose fermenters will appear yellow, and sulfur reducers will appear black. Sulfur reducers could still be enteric.

5. Moderatly selective, differential media.