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14 Cards in this Set
- Front
- Back
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What is the concept behind nitrate reduction test?
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To distinguish bacteria that can reduce nitrate to nitres (or beyond).
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Why would bacteria that are capable of nitrate reduction need to reduce nitrate?
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In the absence of oxygen, some bacteria can reduce nitrate and utilize it as the final hydrogen acceptor.
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What is the enzyme used to breakdown nitrate?
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Nitrate reductase
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What reagent is used in nitrate reduction test?
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Solution A & B
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When adding reagents and no color was produced, what does that mean?
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Either of the following:
1. Nitrate did not reduce to nitrite 2. Nitrate was reduced past nitrate into ammonia or molecular nitrogen |
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What can be added to the media to breakdown nitrate?
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Zinc
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If adding zinc to the negative results (AFTER solution A & B) and it shows a yellow color, what does that mean?
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Nitrate was reduced past nitrite and on to ammonia or molecular nitrogen; bacteria shows positive for nitrate reduction
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If adding zinc to the negative results (AFTER solution A & B) and it shows a red color, what does that mean?
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Nitrate was never reduced to nitrite; bacteria shows negative to nitrate reduction
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If adding solutions A & B to media and it turns red, what does that mean?
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Nitrate was reduced to nitrite; bacteria is capable of nitrate reduction
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If adding solutions A & B to media and it turns yellow, what does that mean?
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Nitrate was either reduced past nitrite to ammonia or molecular nitrogen, or did not reduce nitrate at all.
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How many drops of reagent is used in the nitrate reduction test?
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5
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Which bacteria had an end product of ammonia or molecular nitrogen?
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P. aeruginosa
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Which bacteria had an end product of NO2?
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E. coli, E. aerogenes, P. vulgaris, S. carnosus, B. subtilis, C.xerosis
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Which bacteria had an end product NO3?
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A. faecalis, E. faecalis, L. lactis
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