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33 Cards in this Set
- Front
- Back
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Enzymes that act outside of the cell
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Exoenzymes or extraceullular enzymes
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Enyzmes that act inside the cell
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Endoenzymes or intracellular enzymes
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Term used to break down higher molecular weight substances by introducing them to water and proper enzymes
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Hydrolysis
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What 2 enzymes are used to break down starch and which breaks down what?
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Amylase - dextrin to maltose
Maltase - maltose to glucose |
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What enzyme is used to breakdown lipid and what bond does it breakdown?
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Lipase - breaks ester bonds
Ester bonds |
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What bond does the enzymes breakdown in starch?
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Glycosidic bonds
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What enzyme breaks down protein and what bond does it break?
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Protease & peptide bonds
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Which enzyme is used to breakdown gelatin?
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Gelatinase
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What are the 4 types of hydrolysis within this lab?
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Starch, lipid, casein, and gelatin
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High molecular weight substance composed of glucose, linked together by glycosidic bonds
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Starch
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What is the intracellular breakdown of starch?
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Energy production through glycolysis
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Name the reagent used in the lab experiment and name the hydrolysis it is used for
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Gram's iodine solution - Starch
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What color is produced when gram's iodine is used?
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Clear zone
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What high molecular weight compound possess large amounts of energy linked together by ester bonds?
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Lipid
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Explain the stages to glycolysis and include the enzymes.
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Starch to Dextrin, Dextrin to Maltose (amylase) , Maltose to Glucose (maltase)
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Explain the stages to lipolysis and include the enzymes.
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Triglycerides to Glycerol & fatty acids (lipases)
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What is the intracellular breakdown to Lipids
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ATP & other synthesis requirements
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What color is produced if a bacteria is capable of lipoysis
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Opaque
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Milk protein macromolecule comosed of amino acids linked together by peptide bonds
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Casein
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What is the term use to breakdown starch to ultimately form glucose?
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Glycolysis
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What is the term used to breakdown lipids?
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Lipolysis
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What is the term used to breakdown protein?
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Proteolysis/Peptonization
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Name the stages a protein goes through during proteolysis/peptonization and include the enzymes
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Protein -> peptone -> polypeptide -> dipeptide -> amino acids
Protease |
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What is the medium color when proteolysis/peptonization occurs?
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Decrease of opacity (clear)
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What is another type of protein is hydrolyzed from collagen and what amino acid does it lack?
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Gelatin - tryptophan
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Name the stages a gelatin goes through during its' hydrolysis and name its enzyme
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Collagen -> Gelatin -> amino acids
Gelatinase |
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If a bacteria is capable of utilizing gelatin, what happens to the media and what happens vice versa?
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Liquefaction - solid media
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Which bacteria was capable of starch hydrolysis?
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Bacillus subtilis (clear zone)
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Which bacteria was capable of of casein hydrolysis?
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Pseudomonas aerugionsa + & Bacillus subtillis +++ (clear zone)
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What is the control color of starch media?
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Brown
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What is the control color of lipid media?
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White/opaque
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What is the substrate used in the lipid agar?
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Triglyceride tributyrin
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What is the control color of the milk agar and what is the substrate?
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White/opaque - Milk
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