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74 Cards in this Set
- Front
- Back
psychophilies
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0-20
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mesophilies
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25-40 (disease causing food spoilage microbes)
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thermophilies
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50-60 heat lovers
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hyperthermophilies
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>90
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psychrotrophs
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20-30 food spoilage microbes can grow at 0
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buffers added to culture media:
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neutralize acids and bases and ex: aa, phos salt and peptones
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fac halophilies
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grow in mod high and low salt concs
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obligate halophilies
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require up to 30% salt conc
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makes proteins (dna, rna)
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nitrogen
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needed to amino acid syn
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sulfur
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for atp, phospholipids, dna, rna
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phosphorus
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needed but cant be syn by microbe (purines and vitamins
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organic growth factors
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required for cell respiration
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oxygen
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-->02 to h202 and oxygen
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SOD
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h202 to water and oxygen
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catalase
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needs o2 for growth and has SOD & CATALASE
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OB. AEROBE
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REQUIRES LOW CONC (depends on host enzymes)
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microphilies
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grows with or without oxygen and has SOD and CATALASE
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FAC ANAEROBE
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grows w o2 and is killed by o2 (lacks both)
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obligate anaerobe
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grows in presence of o2 but doesnt use it (sod)
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aerotolerant anaerobe
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nutrient for growing microbes
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culture media
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exact comp known
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chemically defined
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exact comp unknown
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complex medium
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supressed growth of UNWANTED microbes encourages growth of desired microbes
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selective medium
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distinguiches colonies of desired microbes
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differential medium
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for preliminary isolation--favors growth of particular microbe
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enrichment culture
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thioglycolate for oxygen removel
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reducing medium
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c02 rich environments for capnophilies
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anaerobic jar and chamber
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long ter ways of preserving
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deep freexing (-50 to -95) and lyophilization(freeze drying)
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time for cell to divide and pop double
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gen time
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no growth and enzyme DNA and syn occur
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lag
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sensitive to pennicillin and radiation
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log
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counts colonies on agar after capturing bacteria on filter
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filtration
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determines number of coliforms in 100 ml of water or 100g of food
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MPN
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killing of all forms of life
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sterilization
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destruction of veg forms/pathogens
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disinfection
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antiseptic
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living tissue
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disinfectant
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inert surfaces
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reduce number of microbes to acceptable health levels
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sanitizatioin
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mechanical removal of microbes
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degerming
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moist heat
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denatures protein
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applies mild heat to destroy disease causing and food spoilage microbes
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pasteur.
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salts and sugars force water out of microbes
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osmotic pressure
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changes molecular pressure of carbs and proteins
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high pressure
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drying and disrupts metabl/food preservation
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desiccation
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forms thymine dimmers and damages DNA (UV radiation
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non ionizing radiation
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damages DNA by ionizing and sterilizes
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ionizing radiation
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lysol
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phenols
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chlorhexidine (good antiseptic for skin for surgery)
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biguanides
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microbial growth + =
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not effective
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used for degermind and sanitizing and for dairy
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anionic detergents
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have NH4 -- good for mouth wash
-zephiran -bac against gram + |
cationic dtergents
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used for mechanical removal of microbes
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soap
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___ alc wil dehydrate and preserve microbes (Bad)
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100
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___AGNO3 in newborns eye protects against gonorrheal infection
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1
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CUSO4 copper sulfate
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algicide in fish tanks
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ZNCL2 zing chloride
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in toothpaste
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zinc oxide
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paint and antifungal
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inhibit microbial metabolism
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food preservatives
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nisin and natamycin for
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cheese
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denatures protein by bonding with SH, NH2, COOH AND H2 ex formalin and glutaraldehyde
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aldehyde
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for chloride and iodine
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halogens
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ozone, h202, peracetic acid
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peroxygens
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NA0CL
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active ingredient in chlorox
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denatures protein by bonding with SH, NH2, COOH AND H2 ex formalin and glutaraldehyde
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aldehyde
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HOCL + HCL most effective....
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used in pools
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for chloride and iodine
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halogens
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ozone, h202, peracetic acid
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peroxygens
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NA0CL
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active ingredient in chlorox
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HOCL + HCL most effective....
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used in pools
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tincture of iodine=
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irritates skin
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iodophor
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less irritationg
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many ribosomes translating same code
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polysome or polyribosome
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has initiation enongation and termination
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translation
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