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74 Cards in this Set

  • Front
  • Back
psychophilies
0-20
mesophilies
25-40 (disease causing food spoilage microbes)
thermophilies
50-60 heat lovers
hyperthermophilies
>90
psychrotrophs
20-30 food spoilage microbes can grow at 0
buffers added to culture media:
neutralize acids and bases and ex: aa, phos salt and peptones
fac halophilies
grow in mod high and low salt concs
obligate halophilies
require up to 30% salt conc
makes proteins (dna, rna)
nitrogen
needed to amino acid syn
sulfur
for atp, phospholipids, dna, rna
phosphorus
needed but cant be syn by microbe (purines and vitamins
organic growth factors
required for cell respiration
oxygen
-->02 to h202 and oxygen
SOD
h202 to water and oxygen
catalase
needs o2 for growth and has SOD & CATALASE
OB. AEROBE
REQUIRES LOW CONC (depends on host enzymes)
microphilies
grows with or without oxygen and has SOD and CATALASE
FAC ANAEROBE
grows w o2 and is killed by o2 (lacks both)
obligate anaerobe
grows in presence of o2 but doesnt use it (sod)
aerotolerant anaerobe
nutrient for growing microbes
culture media
exact comp known
chemically defined
exact comp unknown
complex medium
supressed growth of UNWANTED microbes encourages growth of desired microbes
selective medium
distinguiches colonies of desired microbes
differential medium
for preliminary isolation--favors growth of particular microbe
enrichment culture
thioglycolate for oxygen removel
reducing medium
c02 rich environments for capnophilies
anaerobic jar and chamber
long ter ways of preserving
deep freexing (-50 to -95) and lyophilization(freeze drying)
time for cell to divide and pop double
gen time
no growth and enzyme DNA and syn occur
lag
sensitive to pennicillin and radiation
log
counts colonies on agar after capturing bacteria on filter
filtration
determines number of coliforms in 100 ml of water or 100g of food
MPN
killing of all forms of life
sterilization
destruction of veg forms/pathogens
disinfection
antiseptic
living tissue
disinfectant
inert surfaces
reduce number of microbes to acceptable health levels
sanitizatioin
mechanical removal of microbes
degerming
moist heat
denatures protein
applies mild heat to destroy disease causing and food spoilage microbes
pasteur.
salts and sugars force water out of microbes
osmotic pressure
changes molecular pressure of carbs and proteins
high pressure
drying and disrupts metabl/food preservation
desiccation
forms thymine dimmers and damages DNA (UV radiation
non ionizing radiation
damages DNA by ionizing and sterilizes
ionizing radiation
lysol
phenols
chlorhexidine (good antiseptic for skin for surgery)
biguanides
microbial growth + =
not effective
used for degermind and sanitizing and for dairy
anionic detergents
have NH4 -- good for mouth wash

-zephiran
-bac against gram +
cationic dtergents
used for mechanical removal of microbes
soap
___ alc wil dehydrate and preserve microbes (Bad)
100
___AGNO3 in newborns eye protects against gonorrheal infection
1
CUSO4 copper sulfate
algicide in fish tanks
ZNCL2 zing chloride
in toothpaste
zinc oxide
paint and antifungal
inhibit microbial metabolism
food preservatives
nisin and natamycin for
cheese
denatures protein by bonding with SH, NH2, COOH AND H2 ex formalin and glutaraldehyde
aldehyde
for chloride and iodine
halogens
ozone, h202, peracetic acid
peroxygens
NA0CL
active ingredient in chlorox
denatures protein by bonding with SH, NH2, COOH AND H2 ex formalin and glutaraldehyde
aldehyde
HOCL + HCL most effective....
used in pools
for chloride and iodine
halogens
ozone, h202, peracetic acid
peroxygens
NA0CL
active ingredient in chlorox
HOCL + HCL most effective....
used in pools
tincture of iodine=
irritates skin
iodophor
less irritationg
many ribosomes translating same code
polysome or polyribosome
has initiation enongation and termination
translation