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24 Cards in this Set
- Front
- Back
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How do you describe a colony? (characteristics)
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Size, color, elevation, shape, surface appearance, consistancy, odor.
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What is confluent growth?
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Colonies of growth that all run together.
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What is a pure culture?
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Media that has only one type of bacteria growing on/in it.
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What are isolated colonies?
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Bacterial colonies away from, or set apart from other growth or colonies on plated media.
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What do you record on Day 1?
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The source of the sample, gram stain results, what it was streaked out on (BAP, CNA, MAC).
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What is recorded on Day 2?
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Colonoy morphology on all plates, presumptive ID, set up confirmatory and sensitivity plates.
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What is recorded on Day 3?
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Positive ID, sensitivity test results (I,R,S).
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What do you use to run a CAMP test?
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Staph aureus
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What is enriched media?
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Media formulated to meet the requirements of fastidious pathogens. They have extra nutrients added (blood, serum, egg).
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Give an example of enriched media.
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BAP, Chocolate
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What is selective media?
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Media that contains antibacterial substances that inhibit or kill all but a few types of bacteria. Facilitate isolation of a particular type from a mixed specimen.
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Give an example of selective media.
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MAC, CNA
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What is differential media?
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Media that allows bacteria to be differentiated into groups by biochemical reactions on the medium.
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Give an example of differential media.
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MAC, Mannitol Salt, BAP
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What is enrichment broth?
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Liquid media that either contain nutrients that encourage the growth of desired bacteria or contain inhibitory substances that supporess competition.
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Give an example of enrichment broth.
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Selenit Broth, Trypitic Soy Broth (TSB)
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Which plate do you read the hemolysis from?
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BAP
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What Gram - swarms?
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Proteus
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What Gram - turns clear agars green?
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Pseudomonas
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What Gram - has no growth on MAC?
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Pasteurella
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Is Staph or Strep catalase positive?
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Staph
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Is staph aureus or staph epi coagulase positive?
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Staph aureus
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Is staph aureus or staph epi MSA positive?
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Staph aureus
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How do you know if the CAMP test is positive or negative?
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Positive has an arrow pointing to the Staph aureus.
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