Micro Fall Ii Croup

Front 1

what are the intracellular bugs that cause respiratory things

whats the mnemonic for facultative intracellular

Back 1

1. Mycoplasm
2. Chlamydia (obligate)
3. Francisella
4. Legionella

Some Nasty Bugs May Live FacultativeLy

1 Salmonella
2. Neisseria
3. Brucella
4. Mycobacterium
5. Listeria
4. Fransciella
5. Legionella

Front 2

DNA virus replicates where?
RNA virus replicates where?

Back 2

DNA- nucleus, except poxvirus
RNA- cytoplasm, except influenza and retrovirus

Front 3

what is croup

Back 3

syndrome: hoarsness, fever, barking cough in 6-18 mo kids. Due to laryngotracheobronchitis- complication of URI, constricts the trachea due to laryngeal edema

PIV 1
PIV 2
RSV

Front 4

what commonly causes edema in the larynx as a complication of URI

Back 4

PIV 1
PIV 2
RSV
**causes in that order

**its CROUP- hoarsness, fever, bark cough in 6 mo-18 mo

Front 5

tell me about the PIV

classificaiton
genome
envelope
serotypes

Back 5

Paramyxovirus
NONSEGEMNTED - sense RNA

*virion is enveloped with spikes

**4 serotypes

Front 6

whats teh causative agent for croup

Back 6

PIV 1 > PIV 2 >>>RSV

Front 7

PIV is a paramyxovirus, what are 3 others

Back 7

RSV
Measles
Mumps

Front 8

whats the deal with PIV and an immune response

Back 8

well its a non segmented RNA genome so it has a limited ability to make AG changes. so we should be able to to have a strong reaction and fight it. BUT what happens is you get immunity for like 20 years and then it wanes and as an adult you get a "cold" bc there is still some protection

Front 9

we know there are 4 serotypes of PIV adn that 1 causes croup more often than 2 but what things make the serotypes different

Back 9

1. Hemagglutinin and Neuroaminidase
2. Novel Fusion (F) protein that causes syntitia formation (fuse into one)

Front 10

how is PIV spread and what does it do in the body and what are the clinical manifestation

Back 10

1. droplets from infected ppl
2. Virus replicates in the upper respiratory epithelium (turbinates and ciliated epithelium) and sits there for like 2-6 days and then causes sore throat, harsh cough, SOB, and CROUP!!! croup clues us in to possible PIV infection

Front 11

how does PIV manifest in kids?
Adults

Back 11

Kids: croup, fever, rhinitis, sore throat, SOB

Adults: upper respiratory tract infection

Front 12

what happens in immunocomprimised kids with PIV and RSV

what are 3 examples of immuno comprimised

Back 12

can get mroe severe, adn enter lower respiratory tract

**chemotherapy
**BM recipients
**adults with leukemia

Front 13

we know PIV causes croup and upper respiratory infection, waht are some other complications of it

Back 13

1. Otitis Media- messes with pressure nad clearance from nasopharynx bacteria

2. Parotitis

Front 14

when do we see PIV/Croup

Back 14

Fall adn Winter

**prevalent in kids!!!!!!

Front 15

do we get lifelong immunity with PIV infection

Back 15

nope, it can be seen in adults but we dont get croup sx

- repeat infections are more mild

Front 16

whats a steeple sign

Back 16

its constriction of the trachea that is seen in x ray

Front 17

how is PIV dx

Back 17

direct viral isolation culture from throat swabs, FAB test (

Front 18

how is PIV tx, vaccien?

Back 18

supportive

None vaccine

Front 19

what type of virus is RSV

Back 19

paramyxovirus: - sense ssRNA

so are PIV, measles, mumbs,

Front 20

does RSV have hemaggluntinin and neuroaminidase

Back 20

nope, RSV infects via G protein spike and does cell fusion with an F spike

Just PIV has these hemagglutinin and neurominidase

Front 21

what infects via G protein spike and dose cell fusion with F spike

Back 21

RSV

PIV- uses hemagglutinin and neuroaminidase

Front 22

RSV causes what

Back 22

a LOWER respiratory tract infection (revall PIV is in upper)

**common in infants/kids. can be fatal in infants

Front 23

why is RSV named as such

Back 23

respiratory syntitial virus

**tendency to create syncytia in tissue culture (multinucleated giant cell)

Front 24

what are the stranis of RSV

Back 24

**distinguished based on AG variation in the G protein (G does attachment)

A- predominates and causes more severe disease

B

Front 25

how dose RSV manifest in Adults and infants

Back 25

Adults/Older Kids: URI

InfantsL bronchiolitis, pneumonia, sometimes croup. can be fatal. Excess mucus, narrow/plug bronchi. fever cough dyspnea

Front 26

when do you expect RSV outbreaks
who is it prevalent in?
how is it spread

Back 26

1. Winter

2. Young kids, adults can get reinfected. no lasting immunity- same was seen in PIV

3. PERSON to person contact, fomites

Front 27

what makes a kid more likely to get RSV

Back 27

1. If they were born 6 months b4 RSV season (april-sept) no longer have moms AB

2. Has older siblings

3. Day care

4. Lives in crowded conditinos

5. Was not breast fed

Front 28

lets beat it to death

1. who gets RSV
2. When do they get it
3. tell me about adults nad RSV
4. spread/transmission

Back 28

1. Kids- by the time your are 4 its most likely youve had it

2. winter

3. they get it, dont get super sick and pass it to a baby

4. Person to person, fomites, direct contacts

Front 29

what is the most likely to introduce RSV to a babe

Back 29

a preschool/school aged older sibling

Front 30

what are 3 ways to dx RSV

Back 30

1. Clinical presentation, a kid in the winter who may have croup

2. detection of viral AG- ELISA or immunofloursence. hard to culture must inoculate sample immediatly

3. Serology- 4x increase in AB titer

Front 31

how is RSV tx

Back 31

can use antiviral ribavirin for severe cases

**typically just tx synptomatically

Front 32

what is ribavirin used for

Back 32

its an anti viral used in severe cases of RSV

Front 33

waht is the immunization for RSV like

Back 33

non existant

**can have passive immunization in babues who are breast fed

Front 34

is it easy to prevent RSV

Back 34

all of those snotty nose kids playing all over each other?! NO WAY!!!!

hand wash, etc

Front 35

what is a hemaglutinin test

Back 35

there is Hemagglutinin in PIV (not RSV) so we can agglutinate the RBS with virus

Front 36

RBC + VIRUS =

RBC + VIRUS + antiviral AB from Serum=

Back 36

hemagglutination

Hemagglutiniation inhibition (the virus binds to the AB and RBC are free)

Front 37

tell me about hemagglutinin inhibition

Back 37

RBC Virus and antiviral AB from pt serum are all added and then the RBC DONT clump- the virus binds to the AB

**if blood doesnt clump the pt has AB to the virus

Front 38

in a hmaggluttin in hibition assay what does it mean if...

1. RBC in a button on bottom
2. RBC aggluninate into a mass

Back 38

1. it means there were antiviral AB present so the virus did NOT aggluniate the RBC

2. NO AB in serum, pt NOT infected

Front 39

what does it mean that you need to have a 4x increase in AB titer if infection was present

Back 39

you need to have hemagglutination with a more dilute solution

Front 40

what does the virus control look like for HI assay

Back 40

RBC + VIRUS

hemagglutination

Front 41

what does the serum control look like for HI

Back 41

Serum + RBC (no virus)

button, no agglutination

Front 42

what does the cell control look like for HI

Back 42

RBC only

button, no agglutionation

Front 43

what is the control for HI, adn what will it look like

1. RBC only
2. Serum + RBC NO virus

3. Virus + RBC

Back 43

1. Cell control, button, no agglutination

2. Serum control, button, no agglunination

3. Virus control, hemagglutinination