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56 Cards in this Set

  • Front
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Urinary Tract- testing
use 1st voided morning urine
contaminant free= suprapubic aspiration
sterile collection- culture in 2 hours, refrig. <24 hrs
streak SBA with calibrated loop
-if over 10^5 CFU/ml and less than 3 colonies= work up
-if <10^4 and >3 colonies= probable absence of UTI
then transfer to selective agar for ID
kidneys, renal, pelvis, ureters
result from ascending infection
fever and pain
found in pts. with altered anatomy
sterile
Upper Urinary tract inf
UUT- pathogens
Enterics
S. aureus
Coag neg Staph,
Mycobacteria
Mycoplasm hominis
urinary bladder and urethra
more common
cystitis and urethritis
sympt- frequency, urgency, and dysuria
Lower Urinary tract inf
LUT- pathogens
Enterics
Enterocicci
Coag neg Staph
Chlamydia trachomatis
N. gonorrhea
Ureaplasma urealyticum
Gardnerella vaginalis
nasal, pharynx, larynx, trachea, left and right primary bronchi
mostly viruses
Upper Respiratory Tract Inf
URT- pathogens
most common- acute pharyngitis and acute otitis media
-viral, strep, or diptheroid
Beta strep
S.pyogenes
Cornebacterium diptheria
B. pertussis
C. gonorrhea
Haemophilus
S. aureus
C. trachomatis
Mycoplasma
C. albicans
URT- testing
2 swabs- for culture and antigen detection
SBA and CHOC
right and left pleural cavity, bronchioles, lungs, mediastinum, diaphragm, pleural space
Bronchitis and pneumonia
sterile
Lower Respiratory inf
LRT- pathogens
viral, fungal, bacterial, parasite
S. aureus
S.pnemoniae*
H. influenzae
Klebsiella
Enterics
Moraxella catarrhalis
Legionella
Bordatella
Pseudomonas
Mycobacterium
Anaerober( Fusobacterium and Prevotells)
Mycoplasma
Chlamydia
LRT- testing
sputum
aspirations, bronchial washings, and needle aspirates= sterile
transport and test with in 2 hours
have to have few epithelial cells and may contain WBCs
plate on SBA, MAC, and CHOC
viral testing
infection from normal flora and viruses, fungi, and parasites= STD
asympt- females
disharge, lesions/warts
Vaginitis- females, dischagre and odor
Cervicitis- females, discharge/ lesion on cervix
Genital tract inf
Genital tract- pathogens
Gardnerella vaginalis (with mixed anaerobes)
F. mobiluncus
H. ducreyi
T. pallidum
N. gonorrhea
N. meningitidis
C. trachomatis
C. albicans
T. vaginalis
HSV
Mycoplasma and Ureaplasma
Genital tract- testing
Dacron or Rayon swabs only- use charcoal transport media
Urine- for molecular
Blood
Genital tract- presumptive ID
Vaginosis
gray discharge and clue cells
Genital tract- presumptive ID
Yeast inf.
budding cells in wet mount
Genital tract- presumptive ID
Trichomonas inf.
motility on wet prep
Genital tract- presumptive ID
N. gohorrhea
Martin Lewis media
intracellular diplococci in dscharge
for males only
Genital tract- presumptive ID
Chlamydia
DFA or NTA
Genital tract- presumptive ID
Gardnerella
KOH and ph >4.5
Wounds and Abscesses- pathogens
S. aureus
S. pyogenes
GN Enterics
Enterococcus
Pseudomonas and non ferm.
Anaerobes ( B fragilils, Fusobacterium, Peptostreptococcus, Clotstridium perfringens)
Wounds and Abscesses- testing
cleanse surface thoroughly to limit contamination
sterile aspirations are more acceptable
use ATM for anaerobes
gram stain
plate on MAC, CNA, PEA, and CHOC
brain, spinal cord, cranial nerves
meningitis, encephalitis, abscesses
sterile
CNS inf
CNS- fungi and parasites
C. neoformans and C. immitis
CNS- neonate bact.
group B strep
E. coli
Enterics
Listeria
H. influenzae type B
CNS- <5 year old bacteria
H influenzae type B
N. meningitidis
S. pneumonia
CNS- adult bacteria
N. meningitidis
S. pneumoniae
Listeria
S. aureus
GNR
CNS- Chronic meningitis bact.
M. tuberculosis
Nocardia
Actinomyces
T. pallidum
Borellia
Leptospira
Brucella
Salmonella
Fungi
CNS- testing
lumbar puncture, gram stain, rapid testing
plate on CHOC, SBA, MAC
CNS- viral CSF
neg gram stian
decreased WBC
normal glucose
slight ^ protein
CNS- bacterial CSF
incr. WBC w/ PMNs
decreased glucose
increase protein
CNS- fungal symptoms
increased cranial pressure and personality changes
Upper GI- pathogens
Helicobacter pylori
Lower GI- pathogens
Salmonella
Shigella
E.coli
S. aureus
Campy
Vibrio
Yersinia
C. difficle
B. cereus
C. perfringens
acute onset, 1-6 days
nausea and vomiting, diarrhea, dehydration, fever (bacterial)/ no fever (enterotoxin), ab. cramps, bloody/ watery (enterotoxin) stool
GI tract- symptoms
GI- testing
feces, rectal swab, washing/biopsy
wet mount- Campy
gram stain
selective media- MAC, HE, XLD, Campy
SMAC (E. coli- EHEC= colorless)
CIN- Y. entercolitica
TCBS- V. cholera/ parahaemolyticus
EIA (Elisa)- C. difficle toxin
Urease- H. pylori
fluid trapped in middle ear
mostly children
Ear inf
Ear inf- pathogens
S. aureus
S. pyogenes
P. aeruginosa
GNB
S. pnemonia*
H. influenzae*
Anaerobes
M. catarrhalis
Ear inf-
Otitis media pathogens
S. pneumonia
H. influenzae
S.pyogenes
S. aureus
M. catarrhalis
Ear inf- testing
ear swab- test in 2 hours
middle ear aspirations
gram stain culture > SBA, MAC, CHOC
conjunctivitis, blepharitis, keratitis
caused by bacteria, chlamydia, and viruses
Eye inf
Eye inf-
Conjunctivits
inflammation of conjunctiva
S. pnemoniae
H. inluenzae
S. aureus
H. aegyptus
N. gonorrhea
S. pyogenes
Adenovirus, HSV, and VZV
Eye inf-
Trachoma
C. trachomatis
A,B,C serovars
Eye inf-
Conjunctivits in neonates bact.
Neiserria and Chlamydia
from mother
Eye inf-
Conjunctivits in children bact.
H. influenzae
S. aureus
S. pnemoniae
Adenovirus
Eye inf-
Conjunctivits in adults bact.
S. aureus
S. pneumoniae
Eye inf-
Blepharitis bact
inflam of eyelid
S. aureus
S. epidermidis
S. pyogenes
HSV
Eye inf-
Keratits
inflam of cornea
P. aeruginosa
S. aureus
S. pneumonia*
HSV
Eye inf- testing
scrapings
gram stain, antigen testing
plat on SBA, CHOC, MAC, Thio
acute infections
no normal flora
Sinusitis
Sinusitis- in children
bact
S. pneumonia
H. influenzae
M. catarrhalis
Rhinovirus
Sinusitis- in young adults
bact
H. influenzae
S. pneumoniae
S. pyogenes
M. catarrhalis
Sinusitis- testing
sinus puncture/ aspiration
gram stain
culture- aerobes and anaerobes
on SBA, CHOC, MAC
fever chills, malaise, tachycardia, hyperventilation, toxicity
Blood inf-
Septicemia
Bacteriemia- in blood
Blood inf- testing
clean skin with ETOH and Iodine
multiple collection bottles (Dont underfill!)
take blood culture just as fever starts if possible
Blood culture base- with TSA for aerobic
- add 0.5% cystine for anaerobic
microscopic exam 1st!!
place in automated blood culture instrument
gram stain
plate on SBA, MAC, CHOC
keep for up to 7 days, then no growth reported
Blood inf- pathogens
S. aureus
Coag neg. Staph
Viridans strep
Enterococcus
S. pnemoniae
Beta hemolytic strep
Pseudomonas
Enterics
Anaerobes- Clostridium and Bacteriodes