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56 Cards in this Set
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Urinary Tract- testing
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use 1st voided morning urine
contaminant free= suprapubic aspiration sterile collection- culture in 2 hours, refrig. <24 hrs streak SBA with calibrated loop -if over 10^5 CFU/ml and less than 3 colonies= work up -if <10^4 and >3 colonies= probable absence of UTI then transfer to selective agar for ID |
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kidneys, renal, pelvis, ureters
result from ascending infection fever and pain found in pts. with altered anatomy sterile |
Upper Urinary tract inf
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UUT- pathogens
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Enterics
S. aureus Coag neg Staph, Mycobacteria Mycoplasm hominis |
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urinary bladder and urethra
more common cystitis and urethritis sympt- frequency, urgency, and dysuria |
Lower Urinary tract inf
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LUT- pathogens
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Enterics
Enterocicci Coag neg Staph Chlamydia trachomatis N. gonorrhea Ureaplasma urealyticum Gardnerella vaginalis |
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nasal, pharynx, larynx, trachea, left and right primary bronchi
mostly viruses |
Upper Respiratory Tract Inf
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URT- pathogens
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most common- acute pharyngitis and acute otitis media
-viral, strep, or diptheroid Beta strep S.pyogenes Cornebacterium diptheria B. pertussis C. gonorrhea Haemophilus S. aureus C. trachomatis Mycoplasma C. albicans |
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URT- testing
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2 swabs- for culture and antigen detection
SBA and CHOC |
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right and left pleural cavity, bronchioles, lungs, mediastinum, diaphragm, pleural space
Bronchitis and pneumonia sterile |
Lower Respiratory inf
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LRT- pathogens
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viral, fungal, bacterial, parasite
S. aureus S.pnemoniae* H. influenzae Klebsiella Enterics Moraxella catarrhalis Legionella Bordatella Pseudomonas Mycobacterium Anaerober( Fusobacterium and Prevotells) Mycoplasma Chlamydia |
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LRT- testing
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sputum
aspirations, bronchial washings, and needle aspirates= sterile transport and test with in 2 hours have to have few epithelial cells and may contain WBCs plate on SBA, MAC, and CHOC viral testing |
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infection from normal flora and viruses, fungi, and parasites= STD
asympt- females disharge, lesions/warts Vaginitis- females, dischagre and odor Cervicitis- females, discharge/ lesion on cervix |
Genital tract inf
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Genital tract- pathogens
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Gardnerella vaginalis (with mixed anaerobes)
F. mobiluncus H. ducreyi T. pallidum N. gonorrhea N. meningitidis C. trachomatis C. albicans T. vaginalis HSV Mycoplasma and Ureaplasma |
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Genital tract- testing
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Dacron or Rayon swabs only- use charcoal transport media
Urine- for molecular Blood |
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Genital tract- presumptive ID
Vaginosis |
gray discharge and clue cells
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Genital tract- presumptive ID
Yeast inf. |
budding cells in wet mount
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Genital tract- presumptive ID
Trichomonas inf. |
motility on wet prep
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Genital tract- presumptive ID
N. gohorrhea |
Martin Lewis media
intracellular diplococci in dscharge for males only |
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Genital tract- presumptive ID
Chlamydia |
DFA or NTA
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Genital tract- presumptive ID
Gardnerella |
KOH and ph >4.5
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Wounds and Abscesses- pathogens
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S. aureus
S. pyogenes GN Enterics Enterococcus Pseudomonas and non ferm. Anaerobes ( B fragilils, Fusobacterium, Peptostreptococcus, Clotstridium perfringens) |
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Wounds and Abscesses- testing
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cleanse surface thoroughly to limit contamination
sterile aspirations are more acceptable use ATM for anaerobes gram stain plate on MAC, CNA, PEA, and CHOC |
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brain, spinal cord, cranial nerves
meningitis, encephalitis, abscesses sterile |
CNS inf
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CNS- fungi and parasites
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C. neoformans and C. immitis
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CNS- neonate bact.
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group B strep
E. coli Enterics Listeria H. influenzae type B |
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CNS- <5 year old bacteria
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H influenzae type B
N. meningitidis S. pneumonia |
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CNS- adult bacteria
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N. meningitidis
S. pneumoniae Listeria S. aureus GNR |
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CNS- Chronic meningitis bact.
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M. tuberculosis
Nocardia Actinomyces T. pallidum Borellia Leptospira Brucella Salmonella Fungi |
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CNS- testing
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lumbar puncture, gram stain, rapid testing
plate on CHOC, SBA, MAC |
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CNS- viral CSF
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neg gram stian
decreased WBC normal glucose slight ^ protein |
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CNS- bacterial CSF
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incr. WBC w/ PMNs
decreased glucose increase protein |
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CNS- fungal symptoms
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increased cranial pressure and personality changes
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Upper GI- pathogens
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Helicobacter pylori
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Lower GI- pathogens
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Salmonella
Shigella E.coli S. aureus Campy Vibrio Yersinia C. difficle B. cereus C. perfringens |
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acute onset, 1-6 days
nausea and vomiting, diarrhea, dehydration, fever (bacterial)/ no fever (enterotoxin), ab. cramps, bloody/ watery (enterotoxin) stool |
GI tract- symptoms
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GI- testing
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feces, rectal swab, washing/biopsy
wet mount- Campy gram stain selective media- MAC, HE, XLD, Campy SMAC (E. coli- EHEC= colorless) CIN- Y. entercolitica TCBS- V. cholera/ parahaemolyticus EIA (Elisa)- C. difficle toxin Urease- H. pylori |
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fluid trapped in middle ear
mostly children |
Ear inf
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Ear inf- pathogens
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S. aureus
S. pyogenes P. aeruginosa GNB S. pnemonia* H. influenzae* Anaerobes M. catarrhalis |
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Ear inf-
Otitis media pathogens |
S. pneumonia
H. influenzae S.pyogenes S. aureus M. catarrhalis |
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Ear inf- testing
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ear swab- test in 2 hours
middle ear aspirations gram stain culture > SBA, MAC, CHOC |
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conjunctivitis, blepharitis, keratitis
caused by bacteria, chlamydia, and viruses |
Eye inf
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Eye inf-
Conjunctivits |
inflammation of conjunctiva
S. pnemoniae H. inluenzae S. aureus H. aegyptus N. gonorrhea S. pyogenes Adenovirus, HSV, and VZV |
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Eye inf-
Trachoma |
C. trachomatis
A,B,C serovars |
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Eye inf-
Conjunctivits in neonates bact. |
Neiserria and Chlamydia
from mother |
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Eye inf-
Conjunctivits in children bact. |
H. influenzae
S. aureus S. pnemoniae Adenovirus |
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Eye inf-
Conjunctivits in adults bact. |
S. aureus
S. pneumoniae |
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Eye inf-
Blepharitis bact |
inflam of eyelid
S. aureus S. epidermidis S. pyogenes HSV |
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Eye inf-
Keratits |
inflam of cornea
P. aeruginosa S. aureus S. pneumonia* HSV |
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Eye inf- testing
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scrapings
gram stain, antigen testing plat on SBA, CHOC, MAC, Thio |
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acute infections
no normal flora |
Sinusitis
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Sinusitis- in children
bact |
S. pneumonia
H. influenzae M. catarrhalis Rhinovirus |
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Sinusitis- in young adults
bact |
H. influenzae
S. pneumoniae S. pyogenes M. catarrhalis |
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Sinusitis- testing
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sinus puncture/ aspiration
gram stain culture- aerobes and anaerobes on SBA, CHOC, MAC |
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fever chills, malaise, tachycardia, hyperventilation, toxicity
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Blood inf-
Septicemia Bacteriemia- in blood |
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Blood inf- testing
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clean skin with ETOH and Iodine
multiple collection bottles (Dont underfill!) take blood culture just as fever starts if possible Blood culture base- with TSA for aerobic - add 0.5% cystine for anaerobic microscopic exam 1st!! place in automated blood culture instrument gram stain plate on SBA, MAC, CHOC keep for up to 7 days, then no growth reported |
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Blood inf- pathogens
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S. aureus
Coag neg. Staph Viridans strep Enterococcus S. pnemoniae Beta hemolytic strep Pseudomonas Enterics Anaerobes- Clostridium and Bacteriodes |