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28 Cards in this Set
- Front
- Back
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What is PEA agar media selective for?
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Selects for Gram positive bacteria
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What is columbia CNA blood agar selective for?
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Selects for Gram positive bacteria
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Mannitol-salt agar
What is it selective for? Differential? How does it differentiate? |
Selects for Staphylococci (salt tolerant)
Differentiates staphylococcus aureas--it ferments mannitol and turns it yellow |
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MacConkey (MAC) agar
What is it selective for? Differential? How? |
Selects for Gram negative bacteria
Differentiates lactose fermenters which turn agar dark pink/red (ie. E Coli) |
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EMB (eosin methylene blue)
What is it selective for? Differential? How? |
Selects for gram negative enterics
Differentiates lactose fermenters--turns to metallic green colonies |
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Thayer-Martin agar
What is it selective for? What promotes its growth? |
Neisseria gonorrhorea & meningitidis
Growth promoted by Hb, dextrose |
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XLD (xylose lysine desoxycholate)
What is it selective for? Differential? how (3 things)? |
Selects for salmonella and shigella
Differentiates Salmonella by its xylose fermentation, H2S production and lysine decarboxylation |
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Hektoen-Enteric agar
What is it selective for? Differential (2 things)? How? |
Selects for enterobacteriaceae family (ie. Salmonella and shigella)
Differentiates lactose fermenters from non fermenters Differentiates salmonella using thiosulfate which reduces hydrogen sulfide to a black ppt |
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CIN (cefsulodin-irgasan-novobiocin)
What is it selective for? Colony morphology? |
Selective for Yersinia sp.
Morphology: Red pigmented "bullseye" in middle of each colony |
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Tinsdale agar
What is it selective for? |
Selective for corynebacterium sp.
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TCBS (thiosulfate citrate-bile salts agar)
What is it selective for? What dz are assoc with this? MO? |
Selective for Vibrio sp.
Vibrio sp. responsible for cholera, diarrhea, food poison |
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BCYE (Buffered charcoal yeast extract agar)
What is it selective for? What is the key ingredient? |
Selects for Legionella sp.
Alpha-ketoglutamate is the key |
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Egg yolk agar
What does it select for? Differential? How? Colony morphology characteristic? |
Selects for Clostridium sp.
Differentiates MOs with lecithinase activity via rich phospholipids in medium--forms zones of opalescence around colonies |
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Lowenstein-Jensen agar
Type of medium Selects for what? Key ingredient? |
Egg-based medium
Selects for Mycobacterium sp. Contaminants inhibited by malachite green |
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Middlebrook agar
What does it select for? Key ingredient? More defined version of which other agar? |
Selects for mycobacterium sp.
Contains malachite green more defined than Lowenstein-Jensen agar |
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Bordet-Gengou (BG) agar
What does it select for? |
Selects for Bordetellapertussis
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MES (ureaplasma agar)
What does it select for? What is the key ingredient and what specific cellular feature does it select for? Culture morphology characteristic? |
Selects for ureaplasma urealyticum
Contains horse serum--a cholesterol needed for stabilizing MOs without cell walls Looks like a fried egg |
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Biochemical test: Catalase
Mechanism? Ex of positive test? Negative test? |
Catalase breaks down H2O2 into H2O and O2
Positive test: Staph Negative test: Strep |
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Biochemical test: Coagulase
Mechanism? This test follows what other test and what does it confirm? |
Coagulase binds fibrinogen causing agglutination/clotting
This test follows catalase test to confirm if MO is staph aureus or other staph. (Staph aureas = positive test) |
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Gelatin liquefaction test
Mechanism? Ex of positive test? |
Produces hydrolytic exoenzymes, gelatinases
Ex: P aeruginosa |
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Oxidase test
Mechanism? Ex positive test? What is the positive test end product produced? |
Detects cytochrome oxidase activity
Ex: Neisseria Positive test will produce a purple end product called indophenol |
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Indole test
Mechanism? End product? What reagent is used on it? What color does it turn? Ex positive test? |
Detects tryptophanase
End product indole reacts with yellow Kovacs reagent turning it red |
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DNAse
Mechanism? |
Methyl green dye bound to polymerized DNA--forms a clear zone when broken down by DNAse
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A disc
What activity does it test for? What MOs does it differentiate between? |
Tests bacitracin susceptibility
Differentiates group A steptococci from other groups of beta hemolytic streptococci |
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P disc
What activity does it test for? What MO does it test for? How? What culture morphology is indicative of a positive test? |
Optochin test
Tests for streptococcus pneumoniae which is sensitive to ethyl hydrocupreine Positive tests shows clear zones |
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Urease test
Mechanism? Ex positive test? Characteristic feature of positive test? |
Detects pH change in conversion of urea to ammonia
Ex: proteus sp. Hot pink = positive test |
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Citrate utilization test
Mechanism? Indicator used? Ex positive test? negative test? |
Tests ability to use citrate used as sole carbon source
Will become alkaline if positive, turning bromthymol blue indicator from green to blue Ex positive test: Enterobacter cloacae Ex negative test: E. coli |
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Bile esculin test
Mechanism? What MO does it test for? Positive test indication? |
Group D streptococci/enterococcus reacts with iron salts
Positive test turns orange to black |
