Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
11 Cards in this Set
- Front
- Back
|
Exponential growth pattern
|
average generation time is 30-60 minutes, can be as short as 10-12 mins
|
|
|
Nf = (Ni)2n
|
formula to calculate the size of a population over time.
Nf= total # cells in the population Ni= starting # N= generation # |
|
|
Lag Phase
|
The cells are not yet multiplying at their maximum rate
|
|
|
Exponential Growth (Logarithmic or log) Phase
|
Cells reach the maximum rate of cell division
|
|
|
Stationary Growth Phase
|
cell #s at highest, just trying to maintain what they have in terms of #s. Waste may be a limiting factor.
|
|
|
Death Phase
|
Cells begin to die at an exponential rate.
Run out of nutrients. |
|
|
Label 4 phases of normal growth curve
|
Slide #51
|
|
|
Turbidometry
|
a tube of clear nutrient solution becomes turbid as microbes grow in it
|
|
|
Direct or total cell count
|
counting the number of cells in a sample microscopically
|
|
|
Flow cytometer
|
Cells don't have to be cultured.
Measured and counted electronically with a laser. Fast and accurate. |
|
|
Real-time PCR
|
Quantify cell DNA instead of counting cells. LIMITING: cells don't have to be living to be counted. BENEFIT: Don't have to culture
|
