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33 Cards in this Set
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Purpose: Differentiate group A stretpococci from other beta-hemolytic streptococci Differentiates staphylococci (resistant) from micrococci (sensitive) Principle: Taxo A dsiks are placed on SBA and zones of inhibition are observed, >10mm = sensitive |
Bacitracin Susceptibility |
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What are the positive and negative controls for Bacitracin Susceptibility |
Pos= Stretpococcus pyogenes; Micrococcs luteus Neg= S. agalactiae, Staphylococcus aureus |
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Purpose: Differentiates enterococci & group D streptococci from nongroup D viridans streptococci Priniciple: Determine whether an org can hydrolyze esculin to esculetin Pos=dark brown to black precipitate will form |
Bile Esculin Agar |
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What are Positive and Negative for Bile Esculin Agar |
Pos= Enterococcus faecalis (also pos for 6 1/2 salt) Neg= Escherichia coli, Streptococcus pyogenes |
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Purpose: Differntiate Streptococcus pneumoniae from alpha-hemolytic streptococci Priniciple: Bile or bile salts rapidly lyse pnneumococcal colonies on blood agar want fresh cultures, old may not be as reactive |
Bile Solubility Test |
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What are the Pos and Neg for Bile Solubility Test |
Pos= S. pneumoniae Neg= Enterococcus faecalis |
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Purpose: Differentiate group B streptococci from other streptococci speicies Listeria monocytogenes is also positive Priniciple: A diffusible hemolytic protein (CAMP factor) acts synergistically with the beta-lysin of S. aureus causing enhanced lysis. Pos=arrow head |
CAMP Test |
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What are the pos and neg of CAMP test |
Pos= Streptococcus agalactiae Neg= S. pyogenes |
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Purpose: Differntiates Catalase pos organisms and staphylococcus species from catalase neg streptococcus Priniciple: Catalase enzyme will converty hydrogen peroxide to water and oxygen, producing bubbles |
Catalase Test |
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What are the pos and neg for the catalase test |
Pos= Staphylococcus aureus Neg= Streptococcus pyogenes |
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Purpose: Id an organism's ability to use sodium citrate as the sole source of carbon Priniciple: Bacteria capable of growing on this medium will use citrate and convert ammonium phosphate into ammonia and ammonium hydrozide (alkaline-turns blue) |
Citrate Utilization |
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What are the pos and neg for citrate utilization |
Pos= Enterobacter aerogenes Neg= E. coli |
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Purpose: Differnetiate S. aureus (pos) from staphylococci(neg) Principle Clumping factor reacts with fibrinogen in plasma, producing a clot results will revert back to neg after 24h |
Coagulase Test |
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What are the pos and neg for Coagulase Test |
Pos= S. aureus Neg= S. epidermidis |
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Purpose: Used to visualize the presence and arrangement of flagella for ID motlile bacterial speicies Principle: Stain contains a mordant to visalize flagella need expertiease |
Flagella Stain |
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In the flagella stain, Peritrichous= Polar= Negative= |
Peritrichous= E. coli Polar= Pseudomonas aeruginosa Negative= Klebsella pneumoniae |
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Purpose: ID organisms that produce tryptophanase Principle: Trytophanase will hydrolyze tryptophan to pyruvate, ammonia, and indole Kovac's reagent will react with indonle and produce a red color Indole spot test will produce a blue color for pos |
Indole Production test |
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Using Kovac's method: Pos= Neg= |
Pos= E. coli Neg= Klebsiella pneumoniae |
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Purpose: differentiate members of the Enterobacteriaceae family Principle: MR detects mixed acid fermination (red) takes 48h VP detects the ability of org to convert the acid products to acetoin and 2,3-butanediol (red) Can be pos for one but not both |
Methyl Red and Voges-Proskauer Tests |
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Purpose: Determines whether an enteric org is motile; must posses flagella Principle: semicolid agar is used to visualize a diffuse zone of growth extending from the line of inoculation |
Motility testing |
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What are the pos and neg for Motility? |
Pos= E. coli Neg= S. aureus |
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Purpose:
Determines whether an org can reduce nitrites Priniciple: Org capable of reducing nitrite to nitrogen will not turn a color and will produce gas. Zinc dust is added if broth does not turn red or no gas ` |
Nitrate Reduction |
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Pos and Neg for Nitrate Reduction |
Pos= Proteus mirabilis Neg= Acinetobacter baumanii |
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Purpose: Diff pneumococci (S) from other alpha-streptococci (R) Priniciple: this disk is placed on an inoculated SBA, incubated, and zones are observed <14mm is questionable |
Optochin (P disk) Susceptibility |
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What are pos and neg for Optochin (P disk Susceptibility) |
Pos= Stretpococcus pneumoniae Neg= S. pyogenes |
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Purpose: Determines the presence of cytochrome oxidase activity for enteric bacteria (neg) from other gram negative rods Priniciple: Determines presence of cytochrome oxidase using tetramehtyl-p-phenylenediamine dihydrochloride to indophenol (turns purple) |
Oxidase test |
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What are the pos and neg for Oxidase test |
Pos= Pseudomonas aeruginosa Neg= E. coli |
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Purpose: Presumptive ID of group A streptococci and enterococci Priniciple: Beta-naphthlamine (byproduct of PYR) will turn red in the presence of N,N dimethylamino cinnamaldehyde reagent |
Pyrrolidonyl Arylamidase Test |
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Pos and Neg controls for Pyrrolidonly Arylamidase Test |
Pos= Enterococcus faecalis, Streptococcus pyogenes Neg= S agalactiae |
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Purpose: Determine whether a gram - rod ferments glucose and lactose or sucrose and forms hydrogen sulfide bonds Principle: Yellow butt= glucose Slant yellow= lactose or sucrose Black tube= hydrogen sulfide |
Triple Sugar Iron Agar |
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K/K= K/A with hydrogen sulfide= K/A= |
K/K= Pseudomonas aeruginosa K/A with hydrogen sulfide= Proteus mirabilis K/A= Shigella flexneri |
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Purpose: Diff Haemophilus species Priniciple: these strips/disks ar eplaced on confluent inoculation org grow around disk with right factor for growth |
X and V Factor Tests |
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XV= requires V, grows around V and XV= Requires X, grows around X and XV = \ |
XV= Haemophilus influenzaerequires V, grows around V and XV= H. parainfluenzaeRequires X, grows around X and XV = H. ducreyi |
