Micro Biology Lab Final Exam

Front 1

TSA (Tryptic Soy Agar) used for?

Back 1

• general purpose cultivation of bacteria-solid
  

Front 2

TSB (Tryptic Soy Broth) used for?

Back 2

• general purpose cultivation of bacteria-liquid
  

Front 3

SAB stands for?

Back 3

• sabouraud agar

Front 4

Is SAB (sabouraud agar) selective, differential, both, or neither?

Back 4

• Selective media

Front 5

SAB (sabouraud agar) used for?

Back 5

• General purpose growth of fungi

Front 6

BAP stands for?

Back 6

• Blood Agar Plate

Front 7

Is BAP (Blood Agar Plate) selective, differential, both, or neither?

Back 7

• Differential media

Front 8

BAP tests for?

Back 8

• presence of hemolysis

Front 9

BAP positive reaction?

Back 9

• Evidence of hemolysis

Front 10

Is Thioglycollate Broth selective, differential, both, or neither?

Back 10

• Neither

Front 11

Thioglycollate Broth is a?

Back 11

• Reducing media that contains ingredients that bind O2 allowing the growth of anaerobes

Front 12

Motility Media/Motility Test: Selective, differential, both, or neither?

Back 12

• Differential Media

Front 13

Motility Media positive reaction?

Back 13

• Christmas tree pattern of growth

Front 14

Fermentation broth used in which test?

Back 14

• Carbohydrate fermentation test

Front 15

Four carbohydrates used in carbohydrate fermentation test?

Back 15

1. glucose
2. sucrose
3. lactose
4. mannitol

Front 16

Fermentation Broth: Selective, differential, both, or neither?

Back 16

• Differential

Front 17

Fermentation Broth pH indicator?

Back 17

• Phenol red

Front 18

Fermentation Broth/ carbohydrate fermentation test positive reaction?

Back 18

• Yellow

Front 19

Fermentation broth also contains?

Back 19

• A Durham tube to collect CO2

Front 20

7% NaCl agar: Selective, differential, both, or neither?

Back 20

• Selective Media

Front 21

7% NaCl agar positive reaction?

Back 21

• Presence of growth

Front 22

EMB stands for?

Back 22

• Eosin Methylene Blue

Front 23

EMB agar: Selective, differential, both, or neither?

Back 23

• Both: selective and differential media

Front 24

EMB agar positive reaction?

Back 24

• Black, or black-centered colonies meaning lactose was fermented

Front 25

Simmons Citrate Agar tests for?

Back 25

• the ability to utilize sodium citrate

Front 26

Simmons citrate agar pH indicator?

Back 26

• Bromthymol blue

Front 27

Simmons citrate agar positive reaction?

Back 27

• Blue

Front 28

Pseudomonas agar P tests for?

Back 28

• the Identity of Pseudomonas aeruginosa

Front 29

Pseudomonas agar P: selective, differential, both, or neither?

Back 29

• Differential media

Front 30

Pseudomonas agar P positive reaction?

Back 30

• Production of blue/green pigment

Front 31

Pseudomonas agar F tests for?

Back 31

• the Identity of Pseudomonas aeruginosa

Front 32

Pseudomonas agar F: selective, differential, both, or neither?

Back 32

• Differential

Front 33

Pseudomonas agar F positive reaction?

Back 33

• Production of yellow/green pigment

Front 34

OF basal media with glucose used in?

Back 34

• Oxydation-fermentation test

Front 35

OF basal media with glucose: selective, differential, both, or neither?

Back 35

• Differential

Front 36

OF basal media with glucose pH indicator?

Back 36

• Bromthymol blue

Front 37

Of basal media with glucose positive reaction?

Back 37

1. Open tube yellow = oxidation
2. Both tubes yellow = fermentation
3. Neither tube yellow = inert

Front 38

Purpose of OF basal media with glucose test?

Back 38

• To differentiate between enterics and non-enterics

Front 39

OF basal media with glucose has what added to "closed" tubes?

Back 39

• Sterile mineral oil

Front 40

Phenylalanine agar (phenylalanine test): Selective, differential, both, or neither?

Back 40

• Differential media

Front 41

Phenylalanine agar/test positive reaction?

Back 41

• Green

Front 42

Phenylalanine agar reagent? (added after incubation)

Back 42

• Ferric chloride

Front 43

TSI agar stands for

Back 43

• Triple sugar iron agar

Front 44

TSI agar tests for?

Back 44

• production of H2S

Front 45

TSI agar: selective, differential, both, or neither?

Back 45

• Differential media

Front 46

TSI agar pH indicator?

Back 46

• Phenol red

Front 47

TSI agar positive reaction?

Back 47

• Formation of black percipitate

Front 48

Three types of decarboxylation broth?

Back 48

1. lysine
2. arginine
3. ornithine

Front 49

Decarboxylation broth test called?

Back 49

• Decarboxylation of amino acids test

Front 50

Decarboxylation broth: selective, differential, both, or neither?

Back 50

• Differential media

Front 51

Decarboxylation broth pH indicator?

Back 51

• Bromcresol purple

Front 52

Decarboxylation broth positive reaction?

Back 52

• Purple with turbidity

Front 53

Must add_____ to decarboxylation broth before incubation?

Back 53

• Sterile mineral oil

Front 54

Starch agar tests for?

Back 54

• production of amylase

Front 55

Starch agar: selective, differential, both, or neither?

Back 55

• Differential media

Front 56

Starch agar test positive reaction?

Back 56

• Zone of hydrolysis (clearing) surrounding the growth

Front 57

Starch agar test reagent?

Back 57

• Iodine

Front 58

Skim milk agar tests for?

Back 58

• Production of caseinase

Front 59

Skim milk agar: selective, differential, both, or neither?

Back 59

• Differential media

Front 60

Skim milk agar test positive reaction?

Back 60

• Zone of hydrolysis (clearing) surrounding growth

Front 61

DNase test agar with methyl green: selective, differential, both, or neither?

Back 61

• Both: selective and differential media

Front 62

DNase test agar with methyl green positive reaction?

Back 62

• Zone of hydrolysis (clearing) surrounding the growth

Front 63

Purpose of DNase test with methyle green?

Back 63

• To differentiate between Staphylococcus aureus and non-pathogenic staphylococci

Front 64

Tryptone broth used in which test?

Back 64

• Indole test

Front 65

Tryptone broth: selective, differential, both, or neither?

Back 65

• Differential media

Front 66

Indole test positive reaction?

Back 66

• Red

Front 67

Indole test reagent?

Back 67

• Kovacs solution

Front 68

MR/VP broth stands for?

Back 68

• Methyl red test and Voges-Proskauer test

Front 69

MR/VP broth: selective, differential, both, or neither?

Back 69

• Differential media

Front 70

MR/VP broth positive reaction?

Back 70

• MR test = Red
• VP test = Red

Front 71

MR/VP test reagents?

Back 71

• MR test = methyl red
• VP test = VP-A and VP-B

Front 72

MR/VP additional fact?

Back 72

• These are two different tests that use the same media

Front 73

MSA stands for?

Back 73

• Mannitol Salt Agar

Front 74

MSA tests for?

Back 74

• The identification of Staphylococcus aureus
  

Front 75

MSA: selective, differential, both, or neither?

Back 75

• Both: selective and differential media

Front 76

MSA pH indicator?

Back 76

• Phenol Red

Front 77

MSA positive reaction?

Back 77

• Yellow
  

Front 78

Nitrate broth used in which test?

Back 78

• Nitrate reduction test

Front 79

Nitrate broth: selective, differential, both, or neither?

Back 79

• Differential media

Front 80

Nitrate reduction test positive reaction?

Back 80

• Red

Front 81

Nitrate reduction test reagent?

Back 81

• Nitrate reagent A and B

Front 82

Nitrate broth also contains?

Back 82

• Durham tube to collect N2 gas

Front 83

Mueller-Hinton agar used in which test?

Back 83

• Antibiotic susceptibility test

Front 84

Mueller-Hinton agar: selective, differential, both, or neither?

Back 84

• Neither

Front 85

Antibiotic susceptibility test positive reaction?

Back 85

• Zone of inhibition surrounding the antibiotic disk

Front 86

Malonate broth tests for?

Back 86

• The ability to use malonate

Front 87

Malonate broth: selective, differential, both, or neither?

Back 87

• Differential media

Front 88

Malonate broth pH indicator?

Back 88

• Bromthymol blue

Front 89

Malonate broth positive reaction?

Back 89

• Blue

Front 90

Oxidase test: Selective, differential, both, or neither?

Back 90

• Neither

Front 91

Purpose of Oxidase Test?

Back 91

• To separate family Pseudomoniaceae from family Enterobacteriaceae

Front 92

Oxidase test positive reaction?

Back 92

• Blue

Front 93

Oxidase test reagent?

Back 93

• Kovacs solution

Front 94

Oxidase Test additional facts?

Back 94

• No media used for this test
• Results are available within seconds

Front 95

Catalase test: selective, differential, both, or neither?

Back 95

• Neither

Front 96

Purpose of Catalase test?

Back 96

• To differentiate between bacteria that can use oxygen (aerobes and facultative anaerobes) from obligate anaerobes

Front 97

Catalase test positive reaction?

Back 97

• Bubbles form

Front 98

Catalase test reagent?

Back 98

• 30% H2O2 (Hydrogen Peroxide)
  

Front 99

Catalase test additional facts?

Back 99

• There is no media used for this test
• Results are available within seconds

Front 100

Citrated rabit plasma used in which test?

Back 100

• Coagulase test

Front 101

Citrated rabbit plasma: selective, differential, both, or neither?

Back 101

• Neither

Front 102

Citrated Rabbit plasma positive reaction?

Back 102

• Coagulation of citrated rabbit plasma

Front 103

Citrated Rabbit plasma reagent?

Back 103

• Citrated rabbit plasma

Front 104

Citrated Rabbit Plasma additional facts?

Back 104

• There is no media used for this test
• Results are available within seconds

Front 105

Urea agar used in which test?

Back 105

• Urea hydrolysis test/urease test

Front 106

Urea agar: selective, differential, both, or neither?

Back 106

• Differential media

Front 107

Urea agar pH indicator?

Back 107

• Phenol red

Front 108

Urea hydrolysis test positive reaction?

Back 108

• Red (reddish-pink to violet)

Front 109

Example of Gram + bacillus?

Back 109

• Bacillus anthracis

Front 110

Example of Gram- bacillus?

Back 110

• Escherichia coli

Front 111

Example of bacteria positive for capsules?

Back 111

• Klebsiella pneumoniae

Front 112

Example of bacteria positive for endospores?

Back 112

• Bacillus anthracis

Front 113

Example of a gram + coccus?

Back 113

• Staphylococcus aureus

Front 114

Example of bacteria + for acid-fast?

Back 114

• Mycobacterium leprae