Micro Bio 520 Last Lecture!!!!

Front 1

What role(s) does RecA protein play in transformation, conjugation, and generalized transduction? How does this activity compare to its function in the SOS response?

Back 1

1. Transformation- Single stranded invasion

2. Conjugation- Critical with HFR

3. Transduction- Phage partical deposit only way to impact

-SOS- RecA degrades LexA protenase.

Front 2

What is the state of the cell when it is capable of taking up DNA? Do all bacterial cells have this ability? Are there restriction on the type of DNA taken up?

Back 2

competence so they can uptake a specific sequence or non-specific, not all cells are capable but you can make them. No there is no restriction.

Front 3

What DNA's are transferred during transduction by P1 and Lambda phage?

Back 3

P1-phase mistakenly packages random fragments of the host chromosome
(life cycle of lytic phage)

Lambda-phase inserts into specific sites in the host genome. Inaccurate exision leads to the packaging of specific DNA sequence adjacent to the insertion site (lambda)

Front 4

What are the characteristics of the F plasmid that allow it to transfer itself (or the host chromosome) during mating?

Back 4

-Cuts DNA at Origin of replication

-Can incorporate into the chromosome by homologous recombination to form and Hfr strain, mobilization and transfer of the F plasmid in an Hfr strain leads to transfer of the chromosome to the recipient strain.

Front 5

How does the structure and gene content of an insertion sequence elements different from the class I and II transposons?

Back 5

II- move entire sequence end to end

I- only one needs to be active