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25 Cards in this Set

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Morphology of Streptococcus
Gram POS cocci (or short rods), typically found in LONG CHAINS. May be in short chains which become harder to ID (particularly Strep. equi) so look out.
Are there more Strep spp or Staph spp?

Where are Strep found?

What is the O2 req of Strep?
Strep spp

Mucous Membranes and Skin of most vertebrates, reptiles, and fish.

Facultative Anaerobes
Are Strep catalase positive or negative?

What about the Enterococcus catalase test?
Step = Catalase NEG
Staphy = Catalase POS

Enterococcus may be pseudocatalase.
Hemolysis... tell me about it
Have NO diagnostic significance whatsoever for Staphylococcus.

Is a diagnostic tool for Streptococcus!
Alpha Strep
do not lyse erythrocytes, but produce a green discoloration around the colony as hemoglobin changes to methemoglobin.

Most Commensal Strep are Alpha
(referred to as viridan strep)
[viridan = greening]
Beta Strep
lyse erythrocytes - produce clearing zones.

Most Beta-Strep are pathogenic
(and the hemolysis can be part of the PATHOGENESIS too, unlike in Staph)
Name 5 species of Beta Hemolytic Strep
S. pyogenes (human)
S. canis (dogs)
S. equi (horses)
S. zooepidemicus (variant of equi)
S. agalactiae (human)
Gamma Strep
No lysis, most are Non-Pathogenic or Opportunists

Enterococcus is one example.
How are Beta-Strep classified?
Serologically sorted into (Rebecca) LANCEFIELD groups.
20 Groups (A-H, K-V)

Animal Pathogens A,B,C,D,E,G,L,V
What bacteria have no Lancefield group?

Have no serologic subdivision?
S. uberis, S. parauberis, S. pneumoniae

S. equi
Big difference bw Strep and Staph pathogenesis?
Strep are Facultative anaerobes --- usually cause their major infections much deeper than Staph (which harshly infects the skin).

Strep like to get away from the O2 and can be fairly fastidious as well
Virulence Factors for Strep are mostly like Staph
So review the Staph cards!
Difference bw Strep / Staph Capsules?
Staph capsule = polysaccharide, very immunogenic... bring the cells in and KILL THEM

Strep capsule = hyaluronic acid, poorly immunogenic... HIDE!
What is PSA?
Pneumococcal Surface Antigen

Lipoprotein found on S. pneumoniae, S. equi - equi, and S. equi - zooepidemicus

Responsible for binding to cells that line the airways
Most strep of veterinary interest are commensals of ________ and ______.

How are strep transmitted?
Upper Respiratory, and GI tracts

Inhalation, Ingestion, sexually, congenitally, or via Fomites
What does Strep pyogenes cause?

(Group A)
(Human) Strep throat, pyoderma, puerperal fever, scarlet fever, rheumatic fever.... Just take some Penicillin.
What does Strep agalactiae cause?

(Group B)
neonatal sepsis (human)
mastitis (cows)

Treat with Penicillin

GB-Mastitis is on the UP because of the desire for "organic" production.
What test is used to ID Group B Strep?
CAMP Test

Run Staph aureus down center of a blood agar plate. Run test subject perpendicular up to this streak. If an "arrow" forms, the bacteria augmented the hemolytic activity of Staph and it is POSITIVE for CAMP and is a GROUP B STREP
Whats up with Group C Strep?
They infect horses.

S. dysgalactiae - dysgalactiae = mastitis
S. dysgalactiae - equisimilis = suppurative conditions
S equi - equi = strangles
S equi - zooepidemicus = pneumonia
Whats up with Group D Strep?
They are mostly really Enterococcus, not strep.

S. bovis and S. equinus are actually a couple STREP that are Group D Strep.
...however they are actually the same organism... so all in all, Group D is for DUMB!
What is the Group G Strep?
(of vet significance)
S. canis
Infects carnivores (mostly dogs)

-Feline lymphadenitis
-Secondary pneumonia in dogs/cats
-Septicemia in puppies
-Assoc with Toxic Shock and Necrotizing Fasciitis in Dogs
What about Group E (P, U, and V)??
S. porcinus

Cervical lymphadenitis in pigs
And Group R, S, RS, T (and D)??
S. suis

encephalitis, meningitis, arthritis, abortion, and endocarditis in pigs

septicemia in pigs and occasionally birds
Ungroupable strains?
S. uberis - bovine mastitis

S. pnuemoniae - human pneumonia
What organism will be the bane of our existence?

Why?
Enterococcus

They are resistant to EVERYTHING!
Occasionally susceptible to tetracycline, amoxicillin...
they are susceptible to vancomycin (used on human MRSA as a last result)
Vancomycin not used in vet med (because we are not allowed to, they are reserved for human med)