Micrb 265 Quiz 1

Front 1

clean working area with disinfectant _________ before and after working, throw away in paper garbage bags

Back 1

Roccal

Front 2

organisms of concentration______ to ______ will be used, and can be pathogenic

Back 2

10^5 to 10^8/mL

Front 3

proper discard procedures are part of _________

Back 3

aseptic technique

Front 4

plastic bag at front of lab for

Back 4

contaminated paper towels

Front 5

used microscope slides placed in ______

Back 5

metal trays on student bench

Front 6

used pipettes (except Pasteur pipette)

Back 6

plastic beakers tips down on student bench

Front 7

used, not contaminated glassware

Back 7

plastic tray at front

Front 8

used tubes

Back 8

wire baskets in angle position (plastic tray noncontaminated tubes, metal tray contaminated tubes)

Front 9

used Petri dishes

Back 9

double plastic bags at front

Front 10

observed on a culture plate using naked eye

Back 10

colony morphology

Front 11

observed using a microscope under 100X magnification, usually after cell staining

Back 11

cell morphology

Front 12

arises from a single cell after multiplication on medium following incubation

Back 12

colony

Front 13

combined characteristics of a colony

Back 13

colony morphology

Front 14

What is basic shape of the colony

Back 14

form

Front 15

What is cross sectional shape of colony

Back 15

elevation

Front 16

what is magnified shape of the edge of the colony

Back 16

margin

Front 17

how does the surface of the colony appear

Back 17

surface

Front 18

how much light does colony pass through

Back 18

transparency

Front 19

pigmentation?

Back 19

color

Front 20

ability of a microscope to show detail

Back 20

resolution or resolving power

Front 21

show that two points that are close together are actually separate

Back 21

resolution/ resolving power

Front 22

resolving power determined by

Back 22

NA and wavelength of light

Front 23

resolving power =

Back 23

wavelength / (2XNA)

Front 24

wavelength of visible light, usually

Back 24

500-600nm

Front 25

when objective lens is separated from the object by air, the NA cannot be greater than

Back 25

1.0

Front 26

oil has larger _________ than air, thus increasing

Back 26

refractive index, NA

Front 27

NA of immersion lens range

Back 27

1.2-1.4

Front 28

resolving power independent of ________

Back 28

magnification

Front 29

needed to enlarge resolved image so that it can be seen

Back 29

magnification

Front 30

further magnification that does not increase detail, only easier to see

Back 30

empty magnification

Front 31

focuses light on the specimen used for illumination

Back 31

condenser lens system

Front 32

condenser lens system often contains ________, which is adjusted so that the circle of the light passing through the condenser just fills the objective lens

Back 32

iris diaphragm

Front 33

shape of individual cells

Back 33

cell morphology

Front 34

clusters, pairs, chains, or singles

Back 34

cell arrangement

Front 35

round cell morphology

Back 35

cocci

Front 36

rod cell morphology

Back 36

bacilli

Front 37

budding and appendaged bacteria

Back 37

hydra and stalk

Front 38

reporting a gram stain

Back 38

gram+/-, cell morphology, cell arrangment

Front 39

gram positive observation

Back 39

purple

Front 40

gram negative observation

Back 40

pink

Front 41

Gram stain steps

Back 41

1) crystal violet
2) wash water
3) Gram's Iodine
4) wash water
5) EtOH
6) wash water
7) Safranin
8) wash and dry

Front 42

allows viewing of free unfixed microorganisms with good detail

Back 42

phase contrast microscopy

Front 43

phase contrast relies on ______ of light passing through the specimen

Back 43

differential diffraction

Front 44

acts like a glue in a phase contrast microscope, allows cover to stick to glass slide, motility still observed in liquid

Back 44

petroleum jelly

Front 45

vs true motility; jiggling of cells caused by water molecules hitting the cells

Back 45

Brownian motion

Front 46

better resolution than light microscopes, wavelength is much smaller than photons of visible light

Back 46

Electron microscopy, EM

Front 47

EM great for seeing ______

Back 47

viruses

Front 48

used to examine the surface features of intact stained cells or viruses, or to examine very thing slices of cells embedded in a resin, electron beam passes through stained specimen to a detector

Back 48

transmission electron microscope

Front 49

examines surface of intact specifmens, magnifies 1000 to 10000X, reflects off surface, used with thin gold to make it scatter more efficiently

Back 49

scanning electron microscope

Front 50

Gram positive, nonmotile rods

Back 50

Mycobacterium

Front 51

mycobacteria are ____________ because their cell wall contains waxy lipids called mycolic acids that make the cells impermeable to many chemicals and dyes

Back 51

acid-fast bacilli

Front 52

once __________ (red stain) penetrates the cell wall, cannot be removed by acidic organic solvents

Back 52

carbolfuchsin

Front 53

malachite green spore stain

Back 53

Schaeffer-Fulton stain

Front 54

resistant form for survival of the organism in non-optimal environmental conditions

Back 54

endospore

Front 55

two most studied bacteria that form endospores

Back 55

Clostridium and Bacteria

Front 56

water soluble, forced into spore by steam, low affinity for cell material

Back 56

malachite green

Front 57

malachite spore stain counterstain

Back 57

safranin

Front 58

external protein structures too thin to be resolved by light microscopy

Back 58

flagella

Front 59

stain to make flagella visible

Back 59

deposition stain

Front 60

stain precipitate accumulates around flagellum, increasing its apparent diameter and making it visible under light microscope

Back 60

deposition stain

Front 61

polymeric sustance, surrounds cell walls of many bacterial species, perform numerous functions, resistance and adhesion

Back 61

capsule

Front 62

materials are water soluble

Back 62

capsule

Front 63

staining bacteria and background more intensely than ______

Back 63

capsule

Front 64

capsule stain technique stains the area around the capsule with a ________ and a _______ stain will color the cell itself

Back 64

negative acidic stain, basic

Front 65

appears as a white halo between the cell and darker background

Back 65

capsule

Front 66

cells that are red with a green interior structure after malachite staining

Back 66

cells with mature endospores

Front 67

brownish red cells after malachite green stain

Back 67

vegetative cells

Front 68

irregular, rhizoid, spindle (lens), circular, filamentous, punctiform

Back 68

form (colony morphology)

Front 69

pulvinate, umbonate, convex, raised, flat

Back 69

elevation (colony morphology)

Front 70

entire (even), undulate (wavy), filamentous, lobate, erose (serated), curled

Back 70

margin