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44 Cards in this Set
- Front
- Back
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PDA plates
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-trying to grow fungi
-(dusty, in contact with soil) |
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TSA plates
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-encourages growth of bacteria
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prokaryote domains:
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-bacteria
-archaea |
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eukaryotic domain:
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-algae
-fungi -protozoa |
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acellular microorganisms
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-viruses
-viroids -prions |
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smaller the distance=
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better the resolution
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symbiosis
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2 or more unrelated species living in a close relationship
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steps to using phase contrast
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1. focus at 400x
2. iris diaphragm open 3. center and align light 4. view |
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saprophytes
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feed on non-living organic material
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yeasts
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-unicellular fungi
-oval shaped -reproduce asexually (budding) |
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molds
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-multicellular fungi
-filamentous hyphae -reproduce by spores |
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mycorrhizae
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fungi mutualistic relationship with plant roots
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as magnification increases...
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-field of view, depth of view, and working distance decrease
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types of symbiosis
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-parasite (host harmed)
-commensal (no harm, 1 benefits) -mutualistic (both benefit) |
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plasmodium
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-protozoa that causes malaria
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trypanosoma
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-protozoa that causes trypanosomiasis (african sleeping sickness)
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gram positive
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-cells appear purple or blue
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gram negative
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-cells appear red or pink
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gram stains that are variable might mean:
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-too thick of a smear
-procedure wrong -culture too old -culture that is variable |
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gram stain procedure
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1. crystal violet 60 sec
2. gram's iodine 60 sec 3. 95% ethanol 10 sec 4. safranin 30 sec |
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bacillus subtilis
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gram +
rods chains |
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moraxella catarrhalis
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gram -
cocci pairs |
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escherichia coli
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gram -
rods singular |
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micrococcus luteus
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gram +
cocci tetrads |
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rhodospirillum rubrum
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gram -
spirillum singular |
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staphylococcus aureus
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gram +
coccus irregular clusters |
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brownian movement
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shaking or shivering motility
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streaming
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same direction, same speed motility
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polar monotrichous
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one flagellum per pole
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polar lophotrichous
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tuft of flagella on one pole
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bipolar lophotrichous
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tuft of flagella on each pole
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peritrichous
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flagella attached all around the cell
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general purpose media
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support the growth of numerous kinds of bacteria. TSB, LB
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selective media
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contains inhibitors that prevent growth of some bacteria, while allowing growth of others. MAC, CNA
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MAC agar
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-gram negative grows
-also differentiates lactose-fermenting bacteria to non-lactose by different colors. -Lactose-fermenting indicates dark red or purple color (e-coli). can ferment = light pink-gray(m. morg) |
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CNA agar
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gram positive grows
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aerobic
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require oxygen
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microaerophilic
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require oxygen, less though
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facultative aerobe
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-can do with or without oxygen
-grow better with |
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anearobe
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-grow without oxygen
- Strict: oxygen is toxic to these organisms - Aerotolerant: have no use for oxygen |
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Brewer's Thioglycolate Broths
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-indicator methylene blue
- has oxygen=blue - no oxygen=colorless |
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resazurin
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- has oxygen=pink
- no oxygen=colorless |
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lichens
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fungi + algae
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gard plate
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soft plate that tests motility
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