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52 Cards in this Set

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Metabolism
Sum of all chemical reactions inside the cell
Catabolic pathways
Degradative reactions
Energy is generated
Exergonic
Ex. Respiration, break down of glucose
Anabolic Rxs.
Additive reactions
Demands input of energy
Endergonic
Ex. Synthesis of proteins, Peptidoglycan, photosynthesis
Nutrients
Chemicals necessary for metabolism
Carbohydrate metabolism
The breakdown of carbohydrate molecule to produce energy.
Mainly glucose metabolism
Energy released is trapped for photosynthesis
What are the two processes in carbohydrate metabolism?
1. Cellular respiration
2. Fermentation
What step is common to both the processes of glucose catabolism (fermentation and respiration)?
Glycolysis: the oxidation of glucose to pyruvic acid (3 carbon compound
What is fermentation?
The partial oxidation of glucose
What are the products of fermentation?
Organic waste products and less ATP than respiration
What are the 3 major steps of respiration
1. Transition reaction
2. Kreb's cycle
3. ETC
What is the transition reaction?
It's the synthesis of Acetyl coenzyme A from pyruvic acid which involved Decarboxylation
What are other names for the Kreb's cycle?
TCA cycle (tricarboxylic acid) or citric acid cycle
What is the Kreb's cycle
The oxidation of Acetyl coenzymeA to Carbon dioxide
Where does the Kreb's cycle occur in prokaryotic cells?
Cytoplasm
ETC
Oxidation of FADH2 and NADH via a series of electron carriers
The energy released is used to generate enormous ATP molecules via oxidative phosphorylation.
In the respiration the final e- acceptor is always an ______________ .
inorganic molecule
What are the two types of respiration?
Aerobic - uses oxygen
Anaerobic- does not use oxygen
What is the final electron acceptor in aerobic respiration?
Oxygen
What is the final electron acceptor in anaerobic respiration?
Final electron acceptor is an inorganic molecule other than molecular oxygen
What are examples of inorganic final electron acceptors?
Sodium Thio Sulfate, Nitrogen gas, carbonate.
Where does our source of vitamin K come from?
E. Coli makes vitamin k and is our exclusive source.
What is dehydrogenation?
The removal of hydrogen atom (proton and electron).
Where does Dehydrogenation occur?
In the NADH to FADH2 process. In the Kreb's cycle.
What is the removal of carbon dioxide called?
Decarboxylation
Where does decarboxylation occur in respiration?
First occurs during the transition phase (pyruvic acid to Acetyl coA)
Second time occurs in the Kreb's cycle
What is phosphorylation?
Addition of phosphate group
Where does phosphorylation occur?
1. Substrate level occurs in glycolysis
2. Oxidative phosphorylation occurs in the ETC
3. Photo Phosphorylation in plants
The electron transport chain is ____________ arranged because _____________.
Asymmetrically arranged, because there are two classes peripheral proteins and integral proteins.
The electron transport chain is ____________ arranged because _____________.
Asymmetrically arranged, because there are two classes peripheral proteins and integral proteins.
Describe the two classes of carriers in the ETC
1 accepts only e- the other accepts e- and H+ pair
Which carrier functions as proton pumps?
The one that accepts e- and H+
Where does the ETC occur in prokaryotes?
Plasma membrane which acts like a surrogate mitochondria
Where does the ETC occur in eukaryotes?
Mitochondria
How does the ETC generate ATP?
The electron is moving from a high energy state to a low energy state and kinetic energy is released which is used to synthesize ATP.
What are the four classes of electron carriers?
Flavoproteins, metal containing proteins, ubiquinone, cytochromes.
F.M.U.C.
Flavoproteins
Integral membrane proteins. Have flavones as their co-enzyme. Oscillates between oxidized/reduced state
Metal containing proteins
Integral membrane proteins in the middle they have metal, iron, sulfur, copper etc.
Ubiquinone
Not a protein. Hydrophobic molecule. Small organic molecules sandwiched between the lipid bi layer, liquid soluble. Present in all living organisms. E. Coli have menaquinone, starting material for vitamin K
Cytochromes
Integral membrane proteins w/heme (we have it in hemoglobin). Heme contains iron.
The loss of e- is called?
Oxidation
The gain of e- is called
Reduction
Why does clostridium perfringens (gangrene) cause necrosis
Because the fermentation products are toxic.
Fermentation uses oxygen (T/F)
False. Fermentation is used by organisms that cannot respire and doesn't use oxygen.
Is the final electron acceptor an organic or inorganic molecule?
An organic molecule (either pyruvate or a derivative of pyruvate)
Fermentation uses oxygen (T/F)
False. Fermentation is used by organisms that cannot respire and doesn't use oxygen.
Is the final electron acceptor in fermentation an organic or inorganic molecule?
An organic molecule (either pyruvate or a derivative of pyruvate)
What is the function of fermentation?
To generate a steady supply of NAD+ for glycolysis so that ADP molecules can be phosphorylase do to ATP.
Fermentation is the _______ oxidation of glucose (sugar) to release energy using an ___________molecule as the final electron acceptor.
Partial, organic
Proteus species can ferment _________ but not __________.
Can ferment glucose but not lactose.
Proteus species can ferment _________ but not __________.
Can ferment glucose but not lactose.
These two bacteria can ferment both glucose and lactose?
E.coli and Enterobacteria.
What causes the "fishy" smell in fish?
TMAO is an oderless nitrogenous waste product of fish metabolism. Some bacteria use TMAO as a final e- acceptor in anaerobic respiration reducing it to TMA. TMA has a very fish odor.