Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
10 Cards in this Set
- Front
- Back
|
What are the Ribosome sizes for Prokaryotes?
|
70S Dissociate into 50s and 30s
|
|
|
What are the Ribosome sizes for Eukaryotes?
|
80S Dissociate into 60s and 40s
|
|
|
Characteristics of Gram Positive Cell Wall
|
1. Thick peptidoglycan layer
2. Teichoic and lipoteichoic acid 3. Multiple Murein layers that prevent the crystal violet from leaving when alcohol is added |
|
|
Characteristic of Gram Negative Cell Wall
|
Two layers - inner thin peptidoglycan
2. Outer membrane contains lipopolysaccharide *Makes more virulent |
|
|
Gram Stain Reagents
|
CIASpy
*Crystal Violet + attracted to - bacteria *Iodine: mordant *Alcohol: Decolorizer; removes dye from only gram neg because gram pos have multiple murein layers. *Safranin - Counterstain *Acid-fast bacteria don't stain well due to waxy and high lipid content in cell wall |
|
|
Endospores
|
Enters vegetative state when environment favors growth (germination)
*Only some gram pos bactera form these |
|
|
Capsules
|
*One of most important virulent factors
*Made of polysaccharides *Covers cell and shields it from immune and phagocytosis |
|
|
Atmosphere (anaerobes)
|
*Obligate Aerobes - Require O2
*Obligate Anaerobes - will not grow in O2 *Facultative anaerobe - With or w/out O2 *Capnophilic - Better in CO2 rich |
|
|
Temperature
|
Psychrophiles - 15C
Mesophiles - 30-35C Thermophiles - 50-60C |
|
|
Growth
|
*LAG - Undected growth
*LOG/Exponential - Constant, maximal growth *Stationary phase - Dying = Dividing Death or Decline - more cells dying |
